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A positive Selection Escherichia Coli Recombinant Protein Expression Vector for One-Step Cloning
We introduce OLIVAR ( O rientation se L ection of I nsert in V ector through A ntisense R eporter) as a novel selection strategy for the insertion of protein-coding genes into vector backbones. As a proof-of-concept, we have engineered a plasmid vector, pGRASS ( G reen fluorescent protein R eporter...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Frontiers Media S.A.
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8761972/ https://www.ncbi.nlm.nih.gov/pubmed/35047486 http://dx.doi.org/10.3389/fbioe.2021.776828 |