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Characterization of XR_311113.2 as a MicroRNA Sponge for Pre-ovulatory Ovarian Follicles of Goats via Long Noncoding RNA Profile and Bioinformatics Analysis

Long noncoding RNAs (lncRNAs) were identified recently as a large class of noncoding RNAs (ncRNAs) with a length ≥200 base pairs (bp). The function and mechanism of lncRNAs have been reported in a growing number of species and tissues. In contrast, the regulatory mechanism of lncRNAs in the goat rep...

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Autores principales: Tao, Hu, Yang, Juan, Zhang, Pengpeng, Zhang, Nian, Suo, Xiaojun, Li, Xiaofeng, Liu, Yang, Chen, Mingxin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8762113/
https://www.ncbi.nlm.nih.gov/pubmed/35046999
http://dx.doi.org/10.3389/fgene.2021.760416
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author Tao, Hu
Yang, Juan
Zhang, Pengpeng
Zhang, Nian
Suo, Xiaojun
Li, Xiaofeng
Liu, Yang
Chen, Mingxin
author_facet Tao, Hu
Yang, Juan
Zhang, Pengpeng
Zhang, Nian
Suo, Xiaojun
Li, Xiaofeng
Liu, Yang
Chen, Mingxin
author_sort Tao, Hu
collection PubMed
description Long noncoding RNAs (lncRNAs) were identified recently as a large class of noncoding RNAs (ncRNAs) with a length ≥200 base pairs (bp). The function and mechanism of lncRNAs have been reported in a growing number of species and tissues. In contrast, the regulatory mechanism of lncRNAs in the goat reproductive system has rarely been reported. In the present study, we sequenced and analyzed the lncRNAs using bioinformatics to identify their expression profiles. As a result, 895 lncRNAs were predicted in the pre-ovulatory ovarian follicles of goats. Eighty-eight lncRNAs were differentially expressed in the Macheng black goat when compared with Boer goat. In addition, the lncRNA XR_311113.2 acted as a sponge of chi-miR-424-5p, as assessed via a luciferase activity assay. Taken together, our findings demonstrate that lncRNAs have potential effects in the ovarian follicles of goats and may represent a promising new research field to understand follicular development.
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spelling pubmed-87621132022-01-18 Characterization of XR_311113.2 as a MicroRNA Sponge for Pre-ovulatory Ovarian Follicles of Goats via Long Noncoding RNA Profile and Bioinformatics Analysis Tao, Hu Yang, Juan Zhang, Pengpeng Zhang, Nian Suo, Xiaojun Li, Xiaofeng Liu, Yang Chen, Mingxin Front Genet Genetics Long noncoding RNAs (lncRNAs) were identified recently as a large class of noncoding RNAs (ncRNAs) with a length ≥200 base pairs (bp). The function and mechanism of lncRNAs have been reported in a growing number of species and tissues. In contrast, the regulatory mechanism of lncRNAs in the goat reproductive system has rarely been reported. In the present study, we sequenced and analyzed the lncRNAs using bioinformatics to identify their expression profiles. As a result, 895 lncRNAs were predicted in the pre-ovulatory ovarian follicles of goats. Eighty-eight lncRNAs were differentially expressed in the Macheng black goat when compared with Boer goat. In addition, the lncRNA XR_311113.2 acted as a sponge of chi-miR-424-5p, as assessed via a luciferase activity assay. Taken together, our findings demonstrate that lncRNAs have potential effects in the ovarian follicles of goats and may represent a promising new research field to understand follicular development. Frontiers Media S.A. 2022-01-03 /pmc/articles/PMC8762113/ /pubmed/35046999 http://dx.doi.org/10.3389/fgene.2021.760416 Text en Copyright © 2022 Tao, Yang, Zhang, Zhang, Suo, Li, Liu and Chen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Tao, Hu
Yang, Juan
Zhang, Pengpeng
Zhang, Nian
Suo, Xiaojun
Li, Xiaofeng
Liu, Yang
Chen, Mingxin
Characterization of XR_311113.2 as a MicroRNA Sponge for Pre-ovulatory Ovarian Follicles of Goats via Long Noncoding RNA Profile and Bioinformatics Analysis
title Characterization of XR_311113.2 as a MicroRNA Sponge for Pre-ovulatory Ovarian Follicles of Goats via Long Noncoding RNA Profile and Bioinformatics Analysis
title_full Characterization of XR_311113.2 as a MicroRNA Sponge for Pre-ovulatory Ovarian Follicles of Goats via Long Noncoding RNA Profile and Bioinformatics Analysis
title_fullStr Characterization of XR_311113.2 as a MicroRNA Sponge for Pre-ovulatory Ovarian Follicles of Goats via Long Noncoding RNA Profile and Bioinformatics Analysis
title_full_unstemmed Characterization of XR_311113.2 as a MicroRNA Sponge for Pre-ovulatory Ovarian Follicles of Goats via Long Noncoding RNA Profile and Bioinformatics Analysis
title_short Characterization of XR_311113.2 as a MicroRNA Sponge for Pre-ovulatory Ovarian Follicles of Goats via Long Noncoding RNA Profile and Bioinformatics Analysis
title_sort characterization of xr_311113.2 as a microrna sponge for pre-ovulatory ovarian follicles of goats via long noncoding rna profile and bioinformatics analysis
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8762113/
https://www.ncbi.nlm.nih.gov/pubmed/35046999
http://dx.doi.org/10.3389/fgene.2021.760416
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