Cargando…

Differential Expression Profiles and Potential Intergenerational Functions of tRNA-Derived Small RNAs in Mice After Cadmium Exposure

Cadmium (Cd) is a toxic heavy metal and ubiquitous environmental endocrine disruptor. Previous studies on Cd-induced damage to male fertility mainly focus on the structure and function of testis, including cytoskeleton, blood-testis barrier, and steroidogenesis. Nevertheless, to date, no studies hav...

Descripción completa

Detalles Bibliográficos
Autores principales: Zeng, Ling, Zhou, Jinzhao, Zhang, Yanwei, Wang, Xiaofei, Wang, Mei, Su, Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8762212/
https://www.ncbi.nlm.nih.gov/pubmed/35047503
http://dx.doi.org/10.3389/fcell.2021.791784
_version_ 1784633710092484608
author Zeng, Ling
Zhou, Jinzhao
Zhang, Yanwei
Wang, Xiaofei
Wang, Mei
Su, Ping
author_facet Zeng, Ling
Zhou, Jinzhao
Zhang, Yanwei
Wang, Xiaofei
Wang, Mei
Su, Ping
author_sort Zeng, Ling
collection PubMed
description Cadmium (Cd) is a toxic heavy metal and ubiquitous environmental endocrine disruptor. Previous studies on Cd-induced damage to male fertility mainly focus on the structure and function of testis, including cytoskeleton, blood-testis barrier, and steroidogenesis. Nevertheless, to date, no studies have investigated the effects of Cd exposure on sperm epigenetic inheritance and intergenerational inheritance. In our study, we systematically revealed the changes in sperm tRNA-derived small RNAs (tsRNA) profiles and found that 14 tsRNAs (9 up-regulated and 5 down-regulated) were significantly altered after Cd exposure. Bioinformatics of tsRNA-mRNA-pathway interactions revealed that the altered biological functions mainly were related to ion transmembrane transport, lipid metabolism and cell membrane system. In addition, we focused on two stages of early embryo development and selected two organs to study the impact of these changes on cell membrane system, especially mitochondrion and lysosome, two typical membrane-enclosed organelles. Surprisingly, we found that the content of mitochondrion was significantly decreased in 2-cell stage, whereas remarkably increased in the morula stage. The contents of mitochondrion and lysosome were increased in the testes of 6-day-old offspring and livers of adult offspring, whereas remarkably decreased in the testes of adult offspring. This provides a possible basis to further explore the effects of paternal Cd exposure on offspring health.
format Online
Article
Text
id pubmed-8762212
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-87622122022-01-18 Differential Expression Profiles and Potential Intergenerational Functions of tRNA-Derived Small RNAs in Mice After Cadmium Exposure Zeng, Ling Zhou, Jinzhao Zhang, Yanwei Wang, Xiaofei Wang, Mei Su, Ping Front Cell Dev Biol Cell and Developmental Biology Cadmium (Cd) is a toxic heavy metal and ubiquitous environmental endocrine disruptor. Previous studies on Cd-induced damage to male fertility mainly focus on the structure and function of testis, including cytoskeleton, blood-testis barrier, and steroidogenesis. Nevertheless, to date, no studies have investigated the effects of Cd exposure on sperm epigenetic inheritance and intergenerational inheritance. In our study, we systematically revealed the changes in sperm tRNA-derived small RNAs (tsRNA) profiles and found that 14 tsRNAs (9 up-regulated and 5 down-regulated) were significantly altered after Cd exposure. Bioinformatics of tsRNA-mRNA-pathway interactions revealed that the altered biological functions mainly were related to ion transmembrane transport, lipid metabolism and cell membrane system. In addition, we focused on two stages of early embryo development and selected two organs to study the impact of these changes on cell membrane system, especially mitochondrion and lysosome, two typical membrane-enclosed organelles. Surprisingly, we found that the content of mitochondrion was significantly decreased in 2-cell stage, whereas remarkably increased in the morula stage. The contents of mitochondrion and lysosome were increased in the testes of 6-day-old offspring and livers of adult offspring, whereas remarkably decreased in the testes of adult offspring. This provides a possible basis to further explore the effects of paternal Cd exposure on offspring health. Frontiers Media S.A. 2022-01-03 /pmc/articles/PMC8762212/ /pubmed/35047503 http://dx.doi.org/10.3389/fcell.2021.791784 Text en Copyright © 2022 Zeng, Zhou, Zhang, Wang, Wang and Su. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Zeng, Ling
Zhou, Jinzhao
Zhang, Yanwei
Wang, Xiaofei
Wang, Mei
Su, Ping
Differential Expression Profiles and Potential Intergenerational Functions of tRNA-Derived Small RNAs in Mice After Cadmium Exposure
title Differential Expression Profiles and Potential Intergenerational Functions of tRNA-Derived Small RNAs in Mice After Cadmium Exposure
title_full Differential Expression Profiles and Potential Intergenerational Functions of tRNA-Derived Small RNAs in Mice After Cadmium Exposure
title_fullStr Differential Expression Profiles and Potential Intergenerational Functions of tRNA-Derived Small RNAs in Mice After Cadmium Exposure
title_full_unstemmed Differential Expression Profiles and Potential Intergenerational Functions of tRNA-Derived Small RNAs in Mice After Cadmium Exposure
title_short Differential Expression Profiles and Potential Intergenerational Functions of tRNA-Derived Small RNAs in Mice After Cadmium Exposure
title_sort differential expression profiles and potential intergenerational functions of trna-derived small rnas in mice after cadmium exposure
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8762212/
https://www.ncbi.nlm.nih.gov/pubmed/35047503
http://dx.doi.org/10.3389/fcell.2021.791784
work_keys_str_mv AT zengling differentialexpressionprofilesandpotentialintergenerationalfunctionsoftrnaderivedsmallrnasinmiceaftercadmiumexposure
AT zhoujinzhao differentialexpressionprofilesandpotentialintergenerationalfunctionsoftrnaderivedsmallrnasinmiceaftercadmiumexposure
AT zhangyanwei differentialexpressionprofilesandpotentialintergenerationalfunctionsoftrnaderivedsmallrnasinmiceaftercadmiumexposure
AT wangxiaofei differentialexpressionprofilesandpotentialintergenerationalfunctionsoftrnaderivedsmallrnasinmiceaftercadmiumexposure
AT wangmei differentialexpressionprofilesandpotentialintergenerationalfunctionsoftrnaderivedsmallrnasinmiceaftercadmiumexposure
AT suping differentialexpressionprofilesandpotentialintergenerationalfunctionsoftrnaderivedsmallrnasinmiceaftercadmiumexposure