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Adipocyte-Specific Inhibition of Mir221/222 Ameliorates Diet-Induced Obesity Through Targeting Ddit4

MicroRNAs expressed in adipocytes are involved in transcriptional regulation of target mRNAs in obesity, but miRNAs critically involved in this process is not well characterized. Here, we identified upregulation of miR-221-3p and miR-222-3p in the white adipose tissues in C57BL/6 mice fed with high...

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Autores principales: Yamaguchi, Satoshi, Zhang, Dongxiao, Katayama, Akihiro, Kurooka, Naoko, Sugawara, Ryosuke, Albuayjan, Haya Hamed Hassan, Nakatsuka, Atsuko, Eguchi, Jun, Wada, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8762293/
https://www.ncbi.nlm.nih.gov/pubmed/35046889
http://dx.doi.org/10.3389/fendo.2021.750261
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author Yamaguchi, Satoshi
Zhang, Dongxiao
Katayama, Akihiro
Kurooka, Naoko
Sugawara, Ryosuke
Albuayjan, Haya Hamed Hassan
Nakatsuka, Atsuko
Eguchi, Jun
Wada, Jun
author_facet Yamaguchi, Satoshi
Zhang, Dongxiao
Katayama, Akihiro
Kurooka, Naoko
Sugawara, Ryosuke
Albuayjan, Haya Hamed Hassan
Nakatsuka, Atsuko
Eguchi, Jun
Wada, Jun
author_sort Yamaguchi, Satoshi
collection PubMed
description MicroRNAs expressed in adipocytes are involved in transcriptional regulation of target mRNAs in obesity, but miRNAs critically involved in this process is not well characterized. Here, we identified upregulation of miR-221-3p and miR-222-3p in the white adipose tissues in C57BL/6 mice fed with high fat-high sucrose (HFHS) chow by RNA sequencing. Mir221 and Mir222 are paralogous genes and share the common seed sequence and Mir221/222AdipoKO mice fed with HFHS chow demonstrated resistance to the development of obesity compared with Mir221/222(flox/y) . Ddit4 is a direct target of Mir221 and Mir222, and the upregulation of Ddit4 in Mir221/222AdipoKO was associated with the suppression of TSC2 (tuberous sclerosis complex 2)/mammalian target of rapamycin complex 1 (mTORC1)/S6K (ribosomal protein S6 kinase) pathway. The overexpression of miR-222-3p linked to enhanced adipogenesis, and it may be a potential candidate for miRNA-based therapy.
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spelling pubmed-87622932022-01-18 Adipocyte-Specific Inhibition of Mir221/222 Ameliorates Diet-Induced Obesity Through Targeting Ddit4 Yamaguchi, Satoshi Zhang, Dongxiao Katayama, Akihiro Kurooka, Naoko Sugawara, Ryosuke Albuayjan, Haya Hamed Hassan Nakatsuka, Atsuko Eguchi, Jun Wada, Jun Front Endocrinol (Lausanne) Endocrinology MicroRNAs expressed in adipocytes are involved in transcriptional regulation of target mRNAs in obesity, but miRNAs critically involved in this process is not well characterized. Here, we identified upregulation of miR-221-3p and miR-222-3p in the white adipose tissues in C57BL/6 mice fed with high fat-high sucrose (HFHS) chow by RNA sequencing. Mir221 and Mir222 are paralogous genes and share the common seed sequence and Mir221/222AdipoKO mice fed with HFHS chow demonstrated resistance to the development of obesity compared with Mir221/222(flox/y) . Ddit4 is a direct target of Mir221 and Mir222, and the upregulation of Ddit4 in Mir221/222AdipoKO was associated with the suppression of TSC2 (tuberous sclerosis complex 2)/mammalian target of rapamycin complex 1 (mTORC1)/S6K (ribosomal protein S6 kinase) pathway. The overexpression of miR-222-3p linked to enhanced adipogenesis, and it may be a potential candidate for miRNA-based therapy. Frontiers Media S.A. 2022-01-03 /pmc/articles/PMC8762293/ /pubmed/35046889 http://dx.doi.org/10.3389/fendo.2021.750261 Text en Copyright © 2022 Yamaguchi, Zhang, Katayama, Kurooka, Sugawara, Albuayjan, Nakatsuka, Eguchi and Wada https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Endocrinology
Yamaguchi, Satoshi
Zhang, Dongxiao
Katayama, Akihiro
Kurooka, Naoko
Sugawara, Ryosuke
Albuayjan, Haya Hamed Hassan
Nakatsuka, Atsuko
Eguchi, Jun
Wada, Jun
Adipocyte-Specific Inhibition of Mir221/222 Ameliorates Diet-Induced Obesity Through Targeting Ddit4
title Adipocyte-Specific Inhibition of Mir221/222 Ameliorates Diet-Induced Obesity Through Targeting Ddit4
title_full Adipocyte-Specific Inhibition of Mir221/222 Ameliorates Diet-Induced Obesity Through Targeting Ddit4
title_fullStr Adipocyte-Specific Inhibition of Mir221/222 Ameliorates Diet-Induced Obesity Through Targeting Ddit4
title_full_unstemmed Adipocyte-Specific Inhibition of Mir221/222 Ameliorates Diet-Induced Obesity Through Targeting Ddit4
title_short Adipocyte-Specific Inhibition of Mir221/222 Ameliorates Diet-Induced Obesity Through Targeting Ddit4
title_sort adipocyte-specific inhibition of mir221/222 ameliorates diet-induced obesity through targeting ddit4
topic Endocrinology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8762293/
https://www.ncbi.nlm.nih.gov/pubmed/35046889
http://dx.doi.org/10.3389/fendo.2021.750261
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