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Hindlimb Immobilization Increases IL-1β and Cdkn2a Expression in Skeletal Muscle Fibro-Adipogenic Progenitor Cells: A Link Between Senescence and Muscle Disuse Atrophy

Loss of muscle mass and strength contributes to decreased independence and an increased risk for morbidity and mortality. A better understanding of the cellular and molecular mechanisms underlying muscle atrophy therefore has significant clinical and therapeutic implications. Fibro-adipogenic progen...

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Autores principales: Parker, Emily, Khayrullin, Andrew, Kent, Andrew, Mendhe, Bharati, Youssef El Baradie, Khairat Bahgat, Yu, Kanglun, Pihkala, Jeanene, Liu, Yutao, McGee-Lawrence, Meghan, Johnson, Maribeth, Chen, Jie, Hamrick, Mark
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8762295/
https://www.ncbi.nlm.nih.gov/pubmed/35047502
http://dx.doi.org/10.3389/fcell.2021.790437
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author Parker, Emily
Khayrullin, Andrew
Kent, Andrew
Mendhe, Bharati
Youssef El Baradie, Khairat Bahgat
Yu, Kanglun
Pihkala, Jeanene
Liu, Yutao
McGee-Lawrence, Meghan
Johnson, Maribeth
Chen, Jie
Hamrick, Mark
author_facet Parker, Emily
Khayrullin, Andrew
Kent, Andrew
Mendhe, Bharati
Youssef El Baradie, Khairat Bahgat
Yu, Kanglun
Pihkala, Jeanene
Liu, Yutao
McGee-Lawrence, Meghan
Johnson, Maribeth
Chen, Jie
Hamrick, Mark
author_sort Parker, Emily
collection PubMed
description Loss of muscle mass and strength contributes to decreased independence and an increased risk for morbidity and mortality. A better understanding of the cellular and molecular mechanisms underlying muscle atrophy therefore has significant clinical and therapeutic implications. Fibro-adipogenic progenitors (FAPs) are a skeletal muscle resident stem cell population that have recently been shown to play vital roles in muscle regeneration and muscle hypertrophy; however, the role that these cells play in muscle disuse atrophy is not well understood. We investigated the role of FAPs in disuse atrophy in vivo utilizing a 2-week single hindlimb immobilization model. RNA-seq was performed on FAPs isolated from the immobilized and non-immobilized limb. The RNAseq data show that IL-1β is significantly upregulated in FAPs following 2 weeks of immobilization, which we confirmed using droplet-digital PCR (ddPCR). We further validated the RNA-seq and ddPCR data from muscle in situ using RNAscope technology. IL-1β is recognized as a key component of the senescence-associated secretory phenotype, or SASP. We then tested the hypothesis that FAPs from the immobilized limb would show elevated senescence measured by cyclin-dependent kinase inhibitor 2A (Cdkn2a) expression as a senescence marker. The ddPCR and RNAscope data both revealed increased Cdkn2a expression in FAPs with immobilization. These data suggest that the gene expression profile of FAPs is significantly altered with disuse, and that disuse itself may drive senescence in FAPs further contributing to muscle atrophy.
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spelling pubmed-87622952022-01-18 Hindlimb Immobilization Increases IL-1β and Cdkn2a Expression in Skeletal Muscle Fibro-Adipogenic Progenitor Cells: A Link Between Senescence and Muscle Disuse Atrophy Parker, Emily Khayrullin, Andrew Kent, Andrew Mendhe, Bharati Youssef El Baradie, Khairat Bahgat Yu, Kanglun Pihkala, Jeanene Liu, Yutao McGee-Lawrence, Meghan Johnson, Maribeth Chen, Jie Hamrick, Mark Front Cell Dev Biol Cell and Developmental Biology Loss of muscle mass and strength contributes to decreased independence and an increased risk for morbidity and mortality. A better understanding of the cellular and molecular mechanisms underlying muscle atrophy therefore has significant clinical and therapeutic implications. Fibro-adipogenic progenitors (FAPs) are a skeletal muscle resident stem cell population that have recently been shown to play vital roles in muscle regeneration and muscle hypertrophy; however, the role that these cells play in muscle disuse atrophy is not well understood. We investigated the role of FAPs in disuse atrophy in vivo utilizing a 2-week single hindlimb immobilization model. RNA-seq was performed on FAPs isolated from the immobilized and non-immobilized limb. The RNAseq data show that IL-1β is significantly upregulated in FAPs following 2 weeks of immobilization, which we confirmed using droplet-digital PCR (ddPCR). We further validated the RNA-seq and ddPCR data from muscle in situ using RNAscope technology. IL-1β is recognized as a key component of the senescence-associated secretory phenotype, or SASP. We then tested the hypothesis that FAPs from the immobilized limb would show elevated senescence measured by cyclin-dependent kinase inhibitor 2A (Cdkn2a) expression as a senescence marker. The ddPCR and RNAscope data both revealed increased Cdkn2a expression in FAPs with immobilization. These data suggest that the gene expression profile of FAPs is significantly altered with disuse, and that disuse itself may drive senescence in FAPs further contributing to muscle atrophy. Frontiers Media S.A. 2022-01-03 /pmc/articles/PMC8762295/ /pubmed/35047502 http://dx.doi.org/10.3389/fcell.2021.790437 Text en Copyright © 2022 Parker, Khayrullin, Kent, Mendhe, Youssef El Baradie, Yu, Pihkala, Liu, McGee-Lawrence, Johnson, Chen and Hamrick. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Parker, Emily
Khayrullin, Andrew
Kent, Andrew
Mendhe, Bharati
Youssef El Baradie, Khairat Bahgat
Yu, Kanglun
Pihkala, Jeanene
Liu, Yutao
McGee-Lawrence, Meghan
Johnson, Maribeth
Chen, Jie
Hamrick, Mark
Hindlimb Immobilization Increases IL-1β and Cdkn2a Expression in Skeletal Muscle Fibro-Adipogenic Progenitor Cells: A Link Between Senescence and Muscle Disuse Atrophy
title Hindlimb Immobilization Increases IL-1β and Cdkn2a Expression in Skeletal Muscle Fibro-Adipogenic Progenitor Cells: A Link Between Senescence and Muscle Disuse Atrophy
title_full Hindlimb Immobilization Increases IL-1β and Cdkn2a Expression in Skeletal Muscle Fibro-Adipogenic Progenitor Cells: A Link Between Senescence and Muscle Disuse Atrophy
title_fullStr Hindlimb Immobilization Increases IL-1β and Cdkn2a Expression in Skeletal Muscle Fibro-Adipogenic Progenitor Cells: A Link Between Senescence and Muscle Disuse Atrophy
title_full_unstemmed Hindlimb Immobilization Increases IL-1β and Cdkn2a Expression in Skeletal Muscle Fibro-Adipogenic Progenitor Cells: A Link Between Senescence and Muscle Disuse Atrophy
title_short Hindlimb Immobilization Increases IL-1β and Cdkn2a Expression in Skeletal Muscle Fibro-Adipogenic Progenitor Cells: A Link Between Senescence and Muscle Disuse Atrophy
title_sort hindlimb immobilization increases il-1β and cdkn2a expression in skeletal muscle fibro-adipogenic progenitor cells: a link between senescence and muscle disuse atrophy
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8762295/
https://www.ncbi.nlm.nih.gov/pubmed/35047502
http://dx.doi.org/10.3389/fcell.2021.790437
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