Determining the metabolic impact of postbiotics in mice

Postbiotics cooperate to influence immune and metabolic outcomes in the host. Here we describe a protocol for in vivo assessment of blood glucose control following acute administration of lipopolysaccharide (LPS) and peptidoglycan (PGN) in mice. This protocol can be adapted for testing a broad range...

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Detalles Bibliográficos
Autores principales: Anhê, Fernando F., Bhatwa, Arshpreet, Schertzer, Jonathan D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8762472/
https://www.ncbi.nlm.nih.gov/pubmed/35072117
http://dx.doi.org/10.1016/j.xpro.2021.101098
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author Anhê, Fernando F.
Bhatwa, Arshpreet
Schertzer, Jonathan D.
author_facet Anhê, Fernando F.
Bhatwa, Arshpreet
Schertzer, Jonathan D.
author_sort Anhê, Fernando F.
collection PubMed
description Postbiotics cooperate to influence immune and metabolic outcomes in the host. Here we describe a protocol for in vivo assessment of blood glucose control following acute administration of lipopolysaccharide (LPS) and peptidoglycan (PGN) in mice. This protocol can be adapted for testing a broad range of microbial molecules and ligands for host immune receptors. Experience with mouse handling is required. For complete details on the use and execution of this protocol, please refer to Anhê et al. (2021) and Cavallari et al. (2017).
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spelling pubmed-87624722022-01-20 Determining the metabolic impact of postbiotics in mice Anhê, Fernando F. Bhatwa, Arshpreet Schertzer, Jonathan D. STAR Protoc Protocol Postbiotics cooperate to influence immune and metabolic outcomes in the host. Here we describe a protocol for in vivo assessment of blood glucose control following acute administration of lipopolysaccharide (LPS) and peptidoglycan (PGN) in mice. This protocol can be adapted for testing a broad range of microbial molecules and ligands for host immune receptors. Experience with mouse handling is required. For complete details on the use and execution of this protocol, please refer to Anhê et al. (2021) and Cavallari et al. (2017). Elsevier 2022-01-13 /pmc/articles/PMC8762472/ /pubmed/35072117 http://dx.doi.org/10.1016/j.xpro.2021.101098 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Anhê, Fernando F.
Bhatwa, Arshpreet
Schertzer, Jonathan D.
Determining the metabolic impact of postbiotics in mice
title Determining the metabolic impact of postbiotics in mice
title_full Determining the metabolic impact of postbiotics in mice
title_fullStr Determining the metabolic impact of postbiotics in mice
title_full_unstemmed Determining the metabolic impact of postbiotics in mice
title_short Determining the metabolic impact of postbiotics in mice
title_sort determining the metabolic impact of postbiotics in mice
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8762472/
https://www.ncbi.nlm.nih.gov/pubmed/35072117
http://dx.doi.org/10.1016/j.xpro.2021.101098
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