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Identification of microRNAs responsive to shear loading in rat skin

Pressure injuries (PIs) are localised skin injuries that result from pressure with or without shear force. Shear force is more destructive than pressure in clinical settings. Therefore, determining the critical external forces is important for selecting the appropriate care to prevent PIs. To quanti...

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Autores principales: Hsu, Wei‐Jhen, Minematsu, Takeo, Nakagami, Gojiro, Koudounas, Sofoklis, Tomida, Sanai, Nakai, Ayano, Kunimitsu, Mao, Nitta, Shiori, Sanada, Hiromi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8762574/
https://www.ncbi.nlm.nih.gov/pubmed/34331398
http://dx.doi.org/10.1111/iwj.13635
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author Hsu, Wei‐Jhen
Minematsu, Takeo
Nakagami, Gojiro
Koudounas, Sofoklis
Tomida, Sanai
Nakai, Ayano
Kunimitsu, Mao
Nitta, Shiori
Sanada, Hiromi
author_facet Hsu, Wei‐Jhen
Minematsu, Takeo
Nakagami, Gojiro
Koudounas, Sofoklis
Tomida, Sanai
Nakai, Ayano
Kunimitsu, Mao
Nitta, Shiori
Sanada, Hiromi
author_sort Hsu, Wei‐Jhen
collection PubMed
description Pressure injuries (PIs) are localised skin injuries that result from pressure with or without shear force. Shear force is more destructive than pressure in clinical settings. Therefore, determining the critical external forces is important for selecting the appropriate care to prevent PIs. To quantitatively distinguish pressure and shear loading with high specificity, we focused on microRNAs (miRs). This study aimed to identify the miRs that are distinguishable between pressure with and without shear loading in rat skin. Microarray analysis identified six candidate miRs from the comparisons among the pressure, shear, and unloaded groups. We analysed the expression levels of the candidate miRs in the process of PI development using real‐time reverse transcriptase polymerase chain reaction. In the pressure and shear groups, miR‐92b expressions at 6 hours after loading were 2.3 ± 1.3 and 2.9 ± 1.0, respectively, which were significantly higher than those in the control group (P = .014 and .004, respectively). miR‐877 expression at 6 hours after loading was significantly increased only in the shear group (2.8 ± 0.9) compared with the control group (P = .016). These results indicate that miR‐92b and miR‐877 are promising biomarkers to determine for which external force healthcare professionals should intervene.
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spelling pubmed-87625742022-01-21 Identification of microRNAs responsive to shear loading in rat skin Hsu, Wei‐Jhen Minematsu, Takeo Nakagami, Gojiro Koudounas, Sofoklis Tomida, Sanai Nakai, Ayano Kunimitsu, Mao Nitta, Shiori Sanada, Hiromi Int Wound J Original Articles Pressure injuries (PIs) are localised skin injuries that result from pressure with or without shear force. Shear force is more destructive than pressure in clinical settings. Therefore, determining the critical external forces is important for selecting the appropriate care to prevent PIs. To quantitatively distinguish pressure and shear loading with high specificity, we focused on microRNAs (miRs). This study aimed to identify the miRs that are distinguishable between pressure with and without shear loading in rat skin. Microarray analysis identified six candidate miRs from the comparisons among the pressure, shear, and unloaded groups. We analysed the expression levels of the candidate miRs in the process of PI development using real‐time reverse transcriptase polymerase chain reaction. In the pressure and shear groups, miR‐92b expressions at 6 hours after loading were 2.3 ± 1.3 and 2.9 ± 1.0, respectively, which were significantly higher than those in the control group (P = .014 and .004, respectively). miR‐877 expression at 6 hours after loading was significantly increased only in the shear group (2.8 ± 0.9) compared with the control group (P = .016). These results indicate that miR‐92b and miR‐877 are promising biomarkers to determine for which external force healthcare professionals should intervene. Blackwell Publishing Ltd 2021-07-31 /pmc/articles/PMC8762574/ /pubmed/34331398 http://dx.doi.org/10.1111/iwj.13635 Text en © 2021 The Authors. International Wound Journal published by Medicalhelplines.com Inc (3M) and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Hsu, Wei‐Jhen
Minematsu, Takeo
Nakagami, Gojiro
Koudounas, Sofoklis
Tomida, Sanai
Nakai, Ayano
Kunimitsu, Mao
Nitta, Shiori
Sanada, Hiromi
Identification of microRNAs responsive to shear loading in rat skin
title Identification of microRNAs responsive to shear loading in rat skin
title_full Identification of microRNAs responsive to shear loading in rat skin
title_fullStr Identification of microRNAs responsive to shear loading in rat skin
title_full_unstemmed Identification of microRNAs responsive to shear loading in rat skin
title_short Identification of microRNAs responsive to shear loading in rat skin
title_sort identification of micrornas responsive to shear loading in rat skin
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8762574/
https://www.ncbi.nlm.nih.gov/pubmed/34331398
http://dx.doi.org/10.1111/iwj.13635
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