A renal cell carcinoma tumorgraft platform to advance precision medicine

Renal cell carcinoma (RCC) encompasses a heterogenous group of tumors, but representative preclinical models are lacking. We previously showed that patient-derived tumorgraft (TG) models recapitulate the biology and treatment responsiveness. Through systematic orthotopic implantation of tumor sample...

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Autores principales: Elias, Roy, Tcheuyap, Vanina T., Kaushik, Akash K., Singla, Nirmish, Gao, Ming, Torras, Oscar Reig, Christie, Alana, Mulgaonkar, Aditi, Woolford, Layton, Stevens, Christina, Kettimuthu, Kavitha Priya, Pavia-Jimenez, Andrea, Boroughs, Lindsey K., Joyce, Allison, Dakanali, Marianna, Notgrass, Hollis, Margulis, Vitaly, Cadeddu, Jeffrey A., Pedrosa, Ivan, Williams, Noelle S., Sun, Xiankai, DeBerardinis, Ralph J., Öz, Orhan K., Zhong, Hua, Seshagiri, Somasekar, Modrusan, Zora, Cantarel, Brandi L., Kapur, Payal, Brugarolas, James
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8762721/
https://www.ncbi.nlm.nih.gov/pubmed/34818533
http://dx.doi.org/10.1016/j.celrep.2021.110055
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author Elias, Roy
Tcheuyap, Vanina T.
Kaushik, Akash K.
Singla, Nirmish
Gao, Ming
Torras, Oscar Reig
Christie, Alana
Mulgaonkar, Aditi
Woolford, Layton
Stevens, Christina
Kettimuthu, Kavitha Priya
Pavia-Jimenez, Andrea
Boroughs, Lindsey K.
Joyce, Allison
Dakanali, Marianna
Notgrass, Hollis
Margulis, Vitaly
Cadeddu, Jeffrey A.
Pedrosa, Ivan
Williams, Noelle S.
Sun, Xiankai
DeBerardinis, Ralph J.
Öz, Orhan K.
Zhong, Hua
Seshagiri, Somasekar
Modrusan, Zora
Cantarel, Brandi L.
Kapur, Payal
Brugarolas, James
author_facet Elias, Roy
Tcheuyap, Vanina T.
Kaushik, Akash K.
Singla, Nirmish
Gao, Ming
Torras, Oscar Reig
Christie, Alana
Mulgaonkar, Aditi
Woolford, Layton
Stevens, Christina
Kettimuthu, Kavitha Priya
Pavia-Jimenez, Andrea
Boroughs, Lindsey K.
Joyce, Allison
Dakanali, Marianna
Notgrass, Hollis
Margulis, Vitaly
Cadeddu, Jeffrey A.
Pedrosa, Ivan
Williams, Noelle S.
Sun, Xiankai
DeBerardinis, Ralph J.
Öz, Orhan K.
Zhong, Hua
Seshagiri, Somasekar
Modrusan, Zora
Cantarel, Brandi L.
Kapur, Payal
Brugarolas, James
author_sort Elias, Roy
collection PubMed
description Renal cell carcinoma (RCC) encompasses a heterogenous group of tumors, but representative preclinical models are lacking. We previously showed that patient-derived tumorgraft (TG) models recapitulate the biology and treatment responsiveness. Through systematic orthotopic implantation of tumor samples from 926 ethnically diverse individuals into non-obese diabetic (NOD)/severe combined immunodeficiency (SCID) mice, we generate a resource comprising 172 independently derived, stably engrafted TG lines from 148 individuals. TG lines are characterized histologically and genomically (whole-exome [n = 97] and RNA [n = 102] sequencing). The platform features a variety of histological and oncogenotypes, including TCGA clades further corroborated through orthogonal metabolomic analyses. We illustrate how it enables a deeper understanding of RCC biology; enables the development of tissue- and imaging-based molecular probes; and supports advances in drug development.
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spelling pubmed-87627212022-01-17 A renal cell carcinoma tumorgraft platform to advance precision medicine Elias, Roy Tcheuyap, Vanina T. Kaushik, Akash K. Singla, Nirmish Gao, Ming Torras, Oscar Reig Christie, Alana Mulgaonkar, Aditi Woolford, Layton Stevens, Christina Kettimuthu, Kavitha Priya Pavia-Jimenez, Andrea Boroughs, Lindsey K. Joyce, Allison Dakanali, Marianna Notgrass, Hollis Margulis, Vitaly Cadeddu, Jeffrey A. Pedrosa, Ivan Williams, Noelle S. Sun, Xiankai DeBerardinis, Ralph J. Öz, Orhan K. Zhong, Hua Seshagiri, Somasekar Modrusan, Zora Cantarel, Brandi L. Kapur, Payal Brugarolas, James Cell Rep Article Renal cell carcinoma (RCC) encompasses a heterogenous group of tumors, but representative preclinical models are lacking. We previously showed that patient-derived tumorgraft (TG) models recapitulate the biology and treatment responsiveness. Through systematic orthotopic implantation of tumor samples from 926 ethnically diverse individuals into non-obese diabetic (NOD)/severe combined immunodeficiency (SCID) mice, we generate a resource comprising 172 independently derived, stably engrafted TG lines from 148 individuals. TG lines are characterized histologically and genomically (whole-exome [n = 97] and RNA [n = 102] sequencing). The platform features a variety of histological and oncogenotypes, including TCGA clades further corroborated through orthogonal metabolomic analyses. We illustrate how it enables a deeper understanding of RCC biology; enables the development of tissue- and imaging-based molecular probes; and supports advances in drug development. 2021-11-23 /pmc/articles/PMC8762721/ /pubmed/34818533 http://dx.doi.org/10.1016/j.celrep.2021.110055 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Article
Elias, Roy
Tcheuyap, Vanina T.
Kaushik, Akash K.
Singla, Nirmish
Gao, Ming
Torras, Oscar Reig
Christie, Alana
Mulgaonkar, Aditi
Woolford, Layton
Stevens, Christina
Kettimuthu, Kavitha Priya
Pavia-Jimenez, Andrea
Boroughs, Lindsey K.
Joyce, Allison
Dakanali, Marianna
Notgrass, Hollis
Margulis, Vitaly
Cadeddu, Jeffrey A.
Pedrosa, Ivan
Williams, Noelle S.
Sun, Xiankai
DeBerardinis, Ralph J.
Öz, Orhan K.
Zhong, Hua
Seshagiri, Somasekar
Modrusan, Zora
Cantarel, Brandi L.
Kapur, Payal
Brugarolas, James
A renal cell carcinoma tumorgraft platform to advance precision medicine
title A renal cell carcinoma tumorgraft platform to advance precision medicine
title_full A renal cell carcinoma tumorgraft platform to advance precision medicine
title_fullStr A renal cell carcinoma tumorgraft platform to advance precision medicine
title_full_unstemmed A renal cell carcinoma tumorgraft platform to advance precision medicine
title_short A renal cell carcinoma tumorgraft platform to advance precision medicine
title_sort renal cell carcinoma tumorgraft platform to advance precision medicine
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8762721/
https://www.ncbi.nlm.nih.gov/pubmed/34818533
http://dx.doi.org/10.1016/j.celrep.2021.110055
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