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Expanding the Benefits of Tnt1 for the Identification of Dominant Mutations in Polyploid Crops: A Single Allelic Mutation in the MsNAC39 Gene Produces Multifoliated Alfalfa

Most major crops are polyploid species and the production of genetically engineered cultivars normally requires the introgression of transgenic or gene-edited traits into elite germplasm. Thus, a main goal of plant research is the search of systems to identify dominant mutations. In this article, we...

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Autores principales: Jozefkowicz, Cintia, Gómez, Cristina, Odorizzi, Ariel, Iantcheva, Anelia, Ratet, Pascal, Ayub, Nicolás, Soto, Gabriela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8763170/
https://www.ncbi.nlm.nih.gov/pubmed/35046986
http://dx.doi.org/10.3389/fpls.2021.805032
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author Jozefkowicz, Cintia
Gómez, Cristina
Odorizzi, Ariel
Iantcheva, Anelia
Ratet, Pascal
Ayub, Nicolás
Soto, Gabriela
author_facet Jozefkowicz, Cintia
Gómez, Cristina
Odorizzi, Ariel
Iantcheva, Anelia
Ratet, Pascal
Ayub, Nicolás
Soto, Gabriela
author_sort Jozefkowicz, Cintia
collection PubMed
description Most major crops are polyploid species and the production of genetically engineered cultivars normally requires the introgression of transgenic or gene-edited traits into elite germplasm. Thus, a main goal of plant research is the search of systems to identify dominant mutations. In this article, we show that the Tnt1 element can be used to identify dominant mutations in allogamous tetraploid cultivated alfalfa. Specifically, we show that a single allelic mutation in the MsNAC39 gene produces multifoliate leaves (mfl) alfalfa plants, a pivot trait of breeding programs of this forage species. Finally, we discuss the potential application of a combination of preliminary screening of beneficial dominant mutants using Tnt1 mutant libraries and genome editing via the CRISPR/Cas9 system to identify target genes and to rapidly improve both autogamous and allogamous polyploid crops.
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spelling pubmed-87631702022-01-18 Expanding the Benefits of Tnt1 for the Identification of Dominant Mutations in Polyploid Crops: A Single Allelic Mutation in the MsNAC39 Gene Produces Multifoliated Alfalfa Jozefkowicz, Cintia Gómez, Cristina Odorizzi, Ariel Iantcheva, Anelia Ratet, Pascal Ayub, Nicolás Soto, Gabriela Front Plant Sci Plant Science Most major crops are polyploid species and the production of genetically engineered cultivars normally requires the introgression of transgenic or gene-edited traits into elite germplasm. Thus, a main goal of plant research is the search of systems to identify dominant mutations. In this article, we show that the Tnt1 element can be used to identify dominant mutations in allogamous tetraploid cultivated alfalfa. Specifically, we show that a single allelic mutation in the MsNAC39 gene produces multifoliate leaves (mfl) alfalfa plants, a pivot trait of breeding programs of this forage species. Finally, we discuss the potential application of a combination of preliminary screening of beneficial dominant mutants using Tnt1 mutant libraries and genome editing via the CRISPR/Cas9 system to identify target genes and to rapidly improve both autogamous and allogamous polyploid crops. Frontiers Media S.A. 2021-12-24 /pmc/articles/PMC8763170/ /pubmed/35046986 http://dx.doi.org/10.3389/fpls.2021.805032 Text en Copyright © 2021 Jozefkowicz, Gómez, Odorizzi, Iantcheva, Ratet, Ayub and Soto. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Jozefkowicz, Cintia
Gómez, Cristina
Odorizzi, Ariel
Iantcheva, Anelia
Ratet, Pascal
Ayub, Nicolás
Soto, Gabriela
Expanding the Benefits of Tnt1 for the Identification of Dominant Mutations in Polyploid Crops: A Single Allelic Mutation in the MsNAC39 Gene Produces Multifoliated Alfalfa
title Expanding the Benefits of Tnt1 for the Identification of Dominant Mutations in Polyploid Crops: A Single Allelic Mutation in the MsNAC39 Gene Produces Multifoliated Alfalfa
title_full Expanding the Benefits of Tnt1 for the Identification of Dominant Mutations in Polyploid Crops: A Single Allelic Mutation in the MsNAC39 Gene Produces Multifoliated Alfalfa
title_fullStr Expanding the Benefits of Tnt1 for the Identification of Dominant Mutations in Polyploid Crops: A Single Allelic Mutation in the MsNAC39 Gene Produces Multifoliated Alfalfa
title_full_unstemmed Expanding the Benefits of Tnt1 for the Identification of Dominant Mutations in Polyploid Crops: A Single Allelic Mutation in the MsNAC39 Gene Produces Multifoliated Alfalfa
title_short Expanding the Benefits of Tnt1 for the Identification of Dominant Mutations in Polyploid Crops: A Single Allelic Mutation in the MsNAC39 Gene Produces Multifoliated Alfalfa
title_sort expanding the benefits of tnt1 for the identification of dominant mutations in polyploid crops: a single allelic mutation in the msnac39 gene produces multifoliated alfalfa
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8763170/
https://www.ncbi.nlm.nih.gov/pubmed/35046986
http://dx.doi.org/10.3389/fpls.2021.805032
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