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Expanding the Benefits of Tnt1 for the Identification of Dominant Mutations in Polyploid Crops: A Single Allelic Mutation in the MsNAC39 Gene Produces Multifoliated Alfalfa
Most major crops are polyploid species and the production of genetically engineered cultivars normally requires the introgression of transgenic or gene-edited traits into elite germplasm. Thus, a main goal of plant research is the search of systems to identify dominant mutations. In this article, we...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8763170/ https://www.ncbi.nlm.nih.gov/pubmed/35046986 http://dx.doi.org/10.3389/fpls.2021.805032 |
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author | Jozefkowicz, Cintia Gómez, Cristina Odorizzi, Ariel Iantcheva, Anelia Ratet, Pascal Ayub, Nicolás Soto, Gabriela |
author_facet | Jozefkowicz, Cintia Gómez, Cristina Odorizzi, Ariel Iantcheva, Anelia Ratet, Pascal Ayub, Nicolás Soto, Gabriela |
author_sort | Jozefkowicz, Cintia |
collection | PubMed |
description | Most major crops are polyploid species and the production of genetically engineered cultivars normally requires the introgression of transgenic or gene-edited traits into elite germplasm. Thus, a main goal of plant research is the search of systems to identify dominant mutations. In this article, we show that the Tnt1 element can be used to identify dominant mutations in allogamous tetraploid cultivated alfalfa. Specifically, we show that a single allelic mutation in the MsNAC39 gene produces multifoliate leaves (mfl) alfalfa plants, a pivot trait of breeding programs of this forage species. Finally, we discuss the potential application of a combination of preliminary screening of beneficial dominant mutants using Tnt1 mutant libraries and genome editing via the CRISPR/Cas9 system to identify target genes and to rapidly improve both autogamous and allogamous polyploid crops. |
format | Online Article Text |
id | pubmed-8763170 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-87631702022-01-18 Expanding the Benefits of Tnt1 for the Identification of Dominant Mutations in Polyploid Crops: A Single Allelic Mutation in the MsNAC39 Gene Produces Multifoliated Alfalfa Jozefkowicz, Cintia Gómez, Cristina Odorizzi, Ariel Iantcheva, Anelia Ratet, Pascal Ayub, Nicolás Soto, Gabriela Front Plant Sci Plant Science Most major crops are polyploid species and the production of genetically engineered cultivars normally requires the introgression of transgenic or gene-edited traits into elite germplasm. Thus, a main goal of plant research is the search of systems to identify dominant mutations. In this article, we show that the Tnt1 element can be used to identify dominant mutations in allogamous tetraploid cultivated alfalfa. Specifically, we show that a single allelic mutation in the MsNAC39 gene produces multifoliate leaves (mfl) alfalfa plants, a pivot trait of breeding programs of this forage species. Finally, we discuss the potential application of a combination of preliminary screening of beneficial dominant mutants using Tnt1 mutant libraries and genome editing via the CRISPR/Cas9 system to identify target genes and to rapidly improve both autogamous and allogamous polyploid crops. Frontiers Media S.A. 2021-12-24 /pmc/articles/PMC8763170/ /pubmed/35046986 http://dx.doi.org/10.3389/fpls.2021.805032 Text en Copyright © 2021 Jozefkowicz, Gómez, Odorizzi, Iantcheva, Ratet, Ayub and Soto. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Jozefkowicz, Cintia Gómez, Cristina Odorizzi, Ariel Iantcheva, Anelia Ratet, Pascal Ayub, Nicolás Soto, Gabriela Expanding the Benefits of Tnt1 for the Identification of Dominant Mutations in Polyploid Crops: A Single Allelic Mutation in the MsNAC39 Gene Produces Multifoliated Alfalfa |
title | Expanding the Benefits of Tnt1 for the Identification of Dominant Mutations in Polyploid Crops: A Single Allelic Mutation in the MsNAC39 Gene Produces Multifoliated Alfalfa |
title_full | Expanding the Benefits of Tnt1 for the Identification of Dominant Mutations in Polyploid Crops: A Single Allelic Mutation in the MsNAC39 Gene Produces Multifoliated Alfalfa |
title_fullStr | Expanding the Benefits of Tnt1 for the Identification of Dominant Mutations in Polyploid Crops: A Single Allelic Mutation in the MsNAC39 Gene Produces Multifoliated Alfalfa |
title_full_unstemmed | Expanding the Benefits of Tnt1 for the Identification of Dominant Mutations in Polyploid Crops: A Single Allelic Mutation in the MsNAC39 Gene Produces Multifoliated Alfalfa |
title_short | Expanding the Benefits of Tnt1 for the Identification of Dominant Mutations in Polyploid Crops: A Single Allelic Mutation in the MsNAC39 Gene Produces Multifoliated Alfalfa |
title_sort | expanding the benefits of tnt1 for the identification of dominant mutations in polyploid crops: a single allelic mutation in the msnac39 gene produces multifoliated alfalfa |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8763170/ https://www.ncbi.nlm.nih.gov/pubmed/35046986 http://dx.doi.org/10.3389/fpls.2021.805032 |
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