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A Novel In Vitro Method to Assess the Microbial Barrier Function of Tissue Adhesives Using Bioluminescence Imaging Technique

Background. Tissue glues can minimize treatment invasiveness, mitigate the risk of infection, and reduce surgery time; ergo, they have been developed and used in surgical procedures as wound closure devices beside sutures, staples, and metallic grafts. Regardless of their structure or function, tiss...

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Autores principales: Mirzaei, Yalda, Hagemeister, Kerstin, Hüffel, Martina, Schwandt, Timo, Tolba, René H., Steitz, Julia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8763484/
https://www.ncbi.nlm.nih.gov/pubmed/35047631
http://dx.doi.org/10.1155/2022/3483238
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author Mirzaei, Yalda
Hagemeister, Kerstin
Hüffel, Martina
Schwandt, Timo
Tolba, René H.
Steitz, Julia
author_facet Mirzaei, Yalda
Hagemeister, Kerstin
Hüffel, Martina
Schwandt, Timo
Tolba, René H.
Steitz, Julia
author_sort Mirzaei, Yalda
collection PubMed
description Background. Tissue glues can minimize treatment invasiveness, mitigate the risk of infection, and reduce surgery time; ergo, they have been developed and used in surgical procedures as wound closure devices beside sutures, staples, and metallic grafts. Regardless of their structure or function, tissue glues should show an acceptable microbial barrier function before being used in humans. This study proposes a novel in vitro method using Escherichia coli Lux and bioluminescence imaging technique to assess the microbial barrier function of tissue glues. Different volumes and concentrations of E. coli Lux were applied to precured or cured polyurethane-based tissue glue placed on agar plates. Plates were cultured for 1 h, 24 h, 48 h, and 72 h with bioluminescence signal measurement subsequently. Herein, protocol established a volume of 5 μL of a 1 : 100 dilution of E. coli Lux containing around 2 × 10(7) CFU/mL as optimal for testing polyurethane-based tissue glue. Measurement of OD(600nm), determination of CFU/mL, and correlation with the bioluminescence measurement in p/s unit resulted in a good correlation between CFU/mL and p/s and demonstrated good reproducibility of our method. In addition, this in vitro method could show that the tested polyurethane-based tissue glue can provide a reasonable barrier against the microbial penetration and act as a bacterial barrier for up to 48 h with no penetration and up to 72 h with a low level of penetration through the material. Overall, we have established a novel, sensitive, and reproducible in vitro method using the bioluminescence imaging technique for testing the microbial barrier function of new tissue glues.
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spelling pubmed-87634842022-01-18 A Novel In Vitro Method to Assess the Microbial Barrier Function of Tissue Adhesives Using Bioluminescence Imaging Technique Mirzaei, Yalda Hagemeister, Kerstin Hüffel, Martina Schwandt, Timo Tolba, René H. Steitz, Julia Biomed Res Int Research Article Background. Tissue glues can minimize treatment invasiveness, mitigate the risk of infection, and reduce surgery time; ergo, they have been developed and used in surgical procedures as wound closure devices beside sutures, staples, and metallic grafts. Regardless of their structure or function, tissue glues should show an acceptable microbial barrier function before being used in humans. This study proposes a novel in vitro method using Escherichia coli Lux and bioluminescence imaging technique to assess the microbial barrier function of tissue glues. Different volumes and concentrations of E. coli Lux were applied to precured or cured polyurethane-based tissue glue placed on agar plates. Plates were cultured for 1 h, 24 h, 48 h, and 72 h with bioluminescence signal measurement subsequently. Herein, protocol established a volume of 5 μL of a 1 : 100 dilution of E. coli Lux containing around 2 × 10(7) CFU/mL as optimal for testing polyurethane-based tissue glue. Measurement of OD(600nm), determination of CFU/mL, and correlation with the bioluminescence measurement in p/s unit resulted in a good correlation between CFU/mL and p/s and demonstrated good reproducibility of our method. In addition, this in vitro method could show that the tested polyurethane-based tissue glue can provide a reasonable barrier against the microbial penetration and act as a bacterial barrier for up to 48 h with no penetration and up to 72 h with a low level of penetration through the material. Overall, we have established a novel, sensitive, and reproducible in vitro method using the bioluminescence imaging technique for testing the microbial barrier function of new tissue glues. Hindawi 2022-01-10 /pmc/articles/PMC8763484/ /pubmed/35047631 http://dx.doi.org/10.1155/2022/3483238 Text en Copyright © 2022 Yalda Mirzaei et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Mirzaei, Yalda
Hagemeister, Kerstin
Hüffel, Martina
Schwandt, Timo
Tolba, René H.
Steitz, Julia
A Novel In Vitro Method to Assess the Microbial Barrier Function of Tissue Adhesives Using Bioluminescence Imaging Technique
title A Novel In Vitro Method to Assess the Microbial Barrier Function of Tissue Adhesives Using Bioluminescence Imaging Technique
title_full A Novel In Vitro Method to Assess the Microbial Barrier Function of Tissue Adhesives Using Bioluminescence Imaging Technique
title_fullStr A Novel In Vitro Method to Assess the Microbial Barrier Function of Tissue Adhesives Using Bioluminescence Imaging Technique
title_full_unstemmed A Novel In Vitro Method to Assess the Microbial Barrier Function of Tissue Adhesives Using Bioluminescence Imaging Technique
title_short A Novel In Vitro Method to Assess the Microbial Barrier Function of Tissue Adhesives Using Bioluminescence Imaging Technique
title_sort novel in vitro method to assess the microbial barrier function of tissue adhesives using bioluminescence imaging technique
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8763484/
https://www.ncbi.nlm.nih.gov/pubmed/35047631
http://dx.doi.org/10.1155/2022/3483238
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