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Multiplexed Genome Editing via an RNA Polymerase II Promoter-Driven sgRNA Array in the Diatom Phaeodactylum tricornutum: Insights Into the Role of StLDP

CRISPR/Cas9-mediated genome editing has been demonstrated in the model diatom P. tricornutum, yet the currently available genetic tools do not combine the various advantageous features into a single, easy-to-assemble, modular construct that would allow the multiplexed targeting and creation of marke...

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Detalles Bibliográficos
Autores principales: Taparia, Yogesh, Dolui, Achintya Kumar, Boussiba, Sammy, Khozin-Goldberg, Inna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8763850/
https://www.ncbi.nlm.nih.gov/pubmed/35058949
http://dx.doi.org/10.3389/fpls.2021.784780