Truncated PARP1 mediates ADP-ribosylation of RNA polymerase III for apoptosis

Caspase-mediated cleavage of PARP1 is a surrogate marker for apoptosis. However, the biological significance of PARP1 cleavage during apoptosis is still unclear. Here, using unbiased protein affinity purification, we show that truncated PARP1 (tPARP1) recognizes the RNA polymerase III (Pol III) comp...

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Autores principales: Chen, Qian, Ma, Kai, Liu, Xiuhua, Chen, Shih-Hsun, Li, Peng, Yu, Yonghao, Leung, Anthony K. L., Yu, Xiaochun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Singapore 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8764063/
https://www.ncbi.nlm.nih.gov/pubmed/35039483
http://dx.doi.org/10.1038/s41421-021-00355-1
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author Chen, Qian
Ma, Kai
Liu, Xiuhua
Chen, Shih-Hsun
Li, Peng
Yu, Yonghao
Leung, Anthony K. L.
Yu, Xiaochun
author_facet Chen, Qian
Ma, Kai
Liu, Xiuhua
Chen, Shih-Hsun
Li, Peng
Yu, Yonghao
Leung, Anthony K. L.
Yu, Xiaochun
author_sort Chen, Qian
collection PubMed
description Caspase-mediated cleavage of PARP1 is a surrogate marker for apoptosis. However, the biological significance of PARP1 cleavage during apoptosis is still unclear. Here, using unbiased protein affinity purification, we show that truncated PARP1 (tPARP1) recognizes the RNA polymerase III (Pol III) complex in the cytosol. tPARP1 mono-ADP-ribosylates RNA Pol III in vitro and mediates ADP-ribosylation of RNA Pol III during poly(dA-dT)-stimulated apoptosis in cells. tPARP1-mediated activation of RNA Pol III facilitates IFN-β production and apoptosis. In contrast, suppression of PARP1 or expressing the non-cleavable form of PARP1 impairs these molecular events. Taken together, these studies reveal a novel biological role of tPARP1 during cytosolic DNA-induced apoptosis.
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spelling pubmed-87640632022-02-04 Truncated PARP1 mediates ADP-ribosylation of RNA polymerase III for apoptosis Chen, Qian Ma, Kai Liu, Xiuhua Chen, Shih-Hsun Li, Peng Yu, Yonghao Leung, Anthony K. L. Yu, Xiaochun Cell Discov Article Caspase-mediated cleavage of PARP1 is a surrogate marker for apoptosis. However, the biological significance of PARP1 cleavage during apoptosis is still unclear. Here, using unbiased protein affinity purification, we show that truncated PARP1 (tPARP1) recognizes the RNA polymerase III (Pol III) complex in the cytosol. tPARP1 mono-ADP-ribosylates RNA Pol III in vitro and mediates ADP-ribosylation of RNA Pol III during poly(dA-dT)-stimulated apoptosis in cells. tPARP1-mediated activation of RNA Pol III facilitates IFN-β production and apoptosis. In contrast, suppression of PARP1 or expressing the non-cleavable form of PARP1 impairs these molecular events. Taken together, these studies reveal a novel biological role of tPARP1 during cytosolic DNA-induced apoptosis. Springer Singapore 2022-01-18 /pmc/articles/PMC8764063/ /pubmed/35039483 http://dx.doi.org/10.1038/s41421-021-00355-1 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Chen, Qian
Ma, Kai
Liu, Xiuhua
Chen, Shih-Hsun
Li, Peng
Yu, Yonghao
Leung, Anthony K. L.
Yu, Xiaochun
Truncated PARP1 mediates ADP-ribosylation of RNA polymerase III for apoptosis
title Truncated PARP1 mediates ADP-ribosylation of RNA polymerase III for apoptosis
title_full Truncated PARP1 mediates ADP-ribosylation of RNA polymerase III for apoptosis
title_fullStr Truncated PARP1 mediates ADP-ribosylation of RNA polymerase III for apoptosis
title_full_unstemmed Truncated PARP1 mediates ADP-ribosylation of RNA polymerase III for apoptosis
title_short Truncated PARP1 mediates ADP-ribosylation of RNA polymerase III for apoptosis
title_sort truncated parp1 mediates adp-ribosylation of rna polymerase iii for apoptosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8764063/
https://www.ncbi.nlm.nih.gov/pubmed/35039483
http://dx.doi.org/10.1038/s41421-021-00355-1
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