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Streptococcus thermophilus extends lifespan through activation of DAF-16-mediated antioxidant pathway in Caenorhabditis elegans

Streptococcus thermophilus bacteria, which are widely used as fermented starter for dairy production, exert various beneficial health effects. Nevertheless, even though pro-longevity effects of various probiotics have been reported, no report has described Streptococcus thermophilus effects on longe...

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Autores principales: Desaka, Natsumi, Ota, Chinatsu, Nishikawa, Hitomi, Yasuda, Kayo, Ishii, Naoaki, Bito, Tomohiro, Kishinaga, Yukio, Naito, Yuji, Higashimura, Yasuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: the Society for Free Radical Research Japan 2022
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8764109/
https://www.ncbi.nlm.nih.gov/pubmed/35068675
http://dx.doi.org/10.3164/jcbn.21-56
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author Desaka, Natsumi
Ota, Chinatsu
Nishikawa, Hitomi
Yasuda, Kayo
Ishii, Naoaki
Bito, Tomohiro
Kishinaga, Yukio
Naito, Yuji
Higashimura, Yasuki
author_facet Desaka, Natsumi
Ota, Chinatsu
Nishikawa, Hitomi
Yasuda, Kayo
Ishii, Naoaki
Bito, Tomohiro
Kishinaga, Yukio
Naito, Yuji
Higashimura, Yasuki
author_sort Desaka, Natsumi
collection PubMed
description Streptococcus thermophilus bacteria, which are widely used as fermented starter for dairy production, exert various beneficial health effects. Nevertheless, even though pro-longevity effects of various probiotics have been reported, no report has described Streptococcus thermophilus effects on longevity. This study was conducted to evaluate Streptococcus thermophilus effects on lifespan extension and to elucidate the Streptococcus thermophilus-mediated longevity mechanism using Caenorhabditis elegans worms as a model animal. They were fed standard food (Escherichia coli OP50) or Streptococcus thermophilus from the young adult stage. Feeding with Streptococcus thermophilus, compared to Escherichia coli OP50, to Caenorhabditis elegans extend the lifespan, reduced lipofuscin accumulation, and maintain vigorous locomotion. Feeding with Streptococcus thermophilus did not alter the worm growth curve or the offspring number, indicating that the Streptococcus thermophilus-mediated lifespan extension is not attributable to caloric restriction. The qRT-PCR data showed that Streptococcus thermophilus increased the expression of daf-16 and some of its downstream antioxidant genes. Furthermore, the pro-longevity effects of Streptococcus thermophilus were decreased in loss-of-function mutant of daf-16. Results show that Streptococcus thermophilus extends the lifespan of Caenorhabditis elegans through DAF-16-mediated antioxidant pathway activation.
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spelling pubmed-87641092022-01-21 Streptococcus thermophilus extends lifespan through activation of DAF-16-mediated antioxidant pathway in Caenorhabditis elegans Desaka, Natsumi Ota, Chinatsu Nishikawa, Hitomi Yasuda, Kayo Ishii, Naoaki Bito, Tomohiro Kishinaga, Yukio Naito, Yuji Higashimura, Yasuki J Clin Biochem Nutr Original Article Streptococcus thermophilus bacteria, which are widely used as fermented starter for dairy production, exert various beneficial health effects. Nevertheless, even though pro-longevity effects of various probiotics have been reported, no report has described Streptococcus thermophilus effects on longevity. This study was conducted to evaluate Streptococcus thermophilus effects on lifespan extension and to elucidate the Streptococcus thermophilus-mediated longevity mechanism using Caenorhabditis elegans worms as a model animal. They were fed standard food (Escherichia coli OP50) or Streptococcus thermophilus from the young adult stage. Feeding with Streptococcus thermophilus, compared to Escherichia coli OP50, to Caenorhabditis elegans extend the lifespan, reduced lipofuscin accumulation, and maintain vigorous locomotion. Feeding with Streptococcus thermophilus did not alter the worm growth curve or the offspring number, indicating that the Streptococcus thermophilus-mediated lifespan extension is not attributable to caloric restriction. The qRT-PCR data showed that Streptococcus thermophilus increased the expression of daf-16 and some of its downstream antioxidant genes. Furthermore, the pro-longevity effects of Streptococcus thermophilus were decreased in loss-of-function mutant of daf-16. Results show that Streptococcus thermophilus extends the lifespan of Caenorhabditis elegans through DAF-16-mediated antioxidant pathway activation. the Society for Free Radical Research Japan 2022-01 2021-07-13 /pmc/articles/PMC8764109/ /pubmed/35068675 http://dx.doi.org/10.3164/jcbn.21-56 Text en Copyright © 2022 JCBN https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Original Article
Desaka, Natsumi
Ota, Chinatsu
Nishikawa, Hitomi
Yasuda, Kayo
Ishii, Naoaki
Bito, Tomohiro
Kishinaga, Yukio
Naito, Yuji
Higashimura, Yasuki
Streptococcus thermophilus extends lifespan through activation of DAF-16-mediated antioxidant pathway in Caenorhabditis elegans
title Streptococcus thermophilus extends lifespan through activation of DAF-16-mediated antioxidant pathway in Caenorhabditis elegans
title_full Streptococcus thermophilus extends lifespan through activation of DAF-16-mediated antioxidant pathway in Caenorhabditis elegans
title_fullStr Streptococcus thermophilus extends lifespan through activation of DAF-16-mediated antioxidant pathway in Caenorhabditis elegans
title_full_unstemmed Streptococcus thermophilus extends lifespan through activation of DAF-16-mediated antioxidant pathway in Caenorhabditis elegans
title_short Streptococcus thermophilus extends lifespan through activation of DAF-16-mediated antioxidant pathway in Caenorhabditis elegans
title_sort streptococcus thermophilus extends lifespan through activation of daf-16-mediated antioxidant pathway in caenorhabditis elegans
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8764109/
https://www.ncbi.nlm.nih.gov/pubmed/35068675
http://dx.doi.org/10.3164/jcbn.21-56
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