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Immunoprofiling Reveals Novel Mast Cell Receptors and the Continuous Nature of Human Lung Mast Cell Heterogeneity

BACKGROUND: Immunohistochemical analysis of granule-associated proteases has revealed that human lung mast cells constitute a heterogeneous population of cells, with distinct subpopulations identified. However, a systematic and comprehensive analysis of cell-surface markers to study human lung mast...

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Autores principales: Rönnberg, Elin, Boey, Daryl Zhong Hao, Ravindran, Avinash, Säfholm, Jesper, Orre, Ann-Charlotte, Al-Ameri, Mamdoh, Adner, Mikael, Dahlén, Sven-Erik, Dahlin, Joakim S., Nilsson, Gunnar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8764255/
https://www.ncbi.nlm.nih.gov/pubmed/35058936
http://dx.doi.org/10.3389/fimmu.2021.804812
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author Rönnberg, Elin
Boey, Daryl Zhong Hao
Ravindran, Avinash
Säfholm, Jesper
Orre, Ann-Charlotte
Al-Ameri, Mamdoh
Adner, Mikael
Dahlén, Sven-Erik
Dahlin, Joakim S.
Nilsson, Gunnar
author_facet Rönnberg, Elin
Boey, Daryl Zhong Hao
Ravindran, Avinash
Säfholm, Jesper
Orre, Ann-Charlotte
Al-Ameri, Mamdoh
Adner, Mikael
Dahlén, Sven-Erik
Dahlin, Joakim S.
Nilsson, Gunnar
author_sort Rönnberg, Elin
collection PubMed
description BACKGROUND: Immunohistochemical analysis of granule-associated proteases has revealed that human lung mast cells constitute a heterogeneous population of cells, with distinct subpopulations identified. However, a systematic and comprehensive analysis of cell-surface markers to study human lung mast cell heterogeneity has yet to be performed. METHODS: Human lung mast cells were obtained from lung lobectomies, and the expression of 332 cell-surface markers was analyzed using flow cytometry and the LEGENDScreen™ kit. Markers that exhibited high variance were selected for additional analyses to reveal whether they were correlated and whether discrete mast cell subpopulations were discernable. RESULTS: We identified the expression of 102 surface markers on human lung mast cells, 23 previously not described on mast cells, of which several showed high continuous variation in their expression. Six of these markers were correlated: SUSD2, CD49a, CD326, CD34, CD66 and HLA-DR. The expression of these markers was also correlated with the size and granularity of mast cells. However, no marker produced an expression profile consistent with a bi- or multimodal distribution. CONCLUSIONS: LEGENDScreen analysis identified more than 100 cell-surface markers on mast cells, including 23 that, to the best of our knowledge, have not been previously described on human mast cells. The comprehensive expression profiling of the 332 surface markers did not identify distinct mast cell subpopulations. Instead, we demonstrate the continuous nature of human lung mast cell heterogeneity.
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spelling pubmed-87642552022-01-19 Immunoprofiling Reveals Novel Mast Cell Receptors and the Continuous Nature of Human Lung Mast Cell Heterogeneity Rönnberg, Elin Boey, Daryl Zhong Hao Ravindran, Avinash Säfholm, Jesper Orre, Ann-Charlotte Al-Ameri, Mamdoh Adner, Mikael Dahlén, Sven-Erik Dahlin, Joakim S. Nilsson, Gunnar Front Immunol Immunology BACKGROUND: Immunohistochemical analysis of granule-associated proteases has revealed that human lung mast cells constitute a heterogeneous population of cells, with distinct subpopulations identified. However, a systematic and comprehensive analysis of cell-surface markers to study human lung mast cell heterogeneity has yet to be performed. METHODS: Human lung mast cells were obtained from lung lobectomies, and the expression of 332 cell-surface markers was analyzed using flow cytometry and the LEGENDScreen™ kit. Markers that exhibited high variance were selected for additional analyses to reveal whether they were correlated and whether discrete mast cell subpopulations were discernable. RESULTS: We identified the expression of 102 surface markers on human lung mast cells, 23 previously not described on mast cells, of which several showed high continuous variation in their expression. Six of these markers were correlated: SUSD2, CD49a, CD326, CD34, CD66 and HLA-DR. The expression of these markers was also correlated with the size and granularity of mast cells. However, no marker produced an expression profile consistent with a bi- or multimodal distribution. CONCLUSIONS: LEGENDScreen analysis identified more than 100 cell-surface markers on mast cells, including 23 that, to the best of our knowledge, have not been previously described on human mast cells. The comprehensive expression profiling of the 332 surface markers did not identify distinct mast cell subpopulations. Instead, we demonstrate the continuous nature of human lung mast cell heterogeneity. Frontiers Media S.A. 2022-01-04 /pmc/articles/PMC8764255/ /pubmed/35058936 http://dx.doi.org/10.3389/fimmu.2021.804812 Text en Copyright © 2022 Rönnberg, Boey, Ravindran, Säfholm, Orre, Al-Ameri, Adner, Dahlén, Dahlin and Nilsson https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Rönnberg, Elin
Boey, Daryl Zhong Hao
Ravindran, Avinash
Säfholm, Jesper
Orre, Ann-Charlotte
Al-Ameri, Mamdoh
Adner, Mikael
Dahlén, Sven-Erik
Dahlin, Joakim S.
Nilsson, Gunnar
Immunoprofiling Reveals Novel Mast Cell Receptors and the Continuous Nature of Human Lung Mast Cell Heterogeneity
title Immunoprofiling Reveals Novel Mast Cell Receptors and the Continuous Nature of Human Lung Mast Cell Heterogeneity
title_full Immunoprofiling Reveals Novel Mast Cell Receptors and the Continuous Nature of Human Lung Mast Cell Heterogeneity
title_fullStr Immunoprofiling Reveals Novel Mast Cell Receptors and the Continuous Nature of Human Lung Mast Cell Heterogeneity
title_full_unstemmed Immunoprofiling Reveals Novel Mast Cell Receptors and the Continuous Nature of Human Lung Mast Cell Heterogeneity
title_short Immunoprofiling Reveals Novel Mast Cell Receptors and the Continuous Nature of Human Lung Mast Cell Heterogeneity
title_sort immunoprofiling reveals novel mast cell receptors and the continuous nature of human lung mast cell heterogeneity
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8764255/
https://www.ncbi.nlm.nih.gov/pubmed/35058936
http://dx.doi.org/10.3389/fimmu.2021.804812
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