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The Route of Vaccine Administration Determines Whether Blood Neutrophils Undergo Long-Term Phenotypic Modifications
Innate immunity modulates adaptive immunity and defines the magnitude, quality, and longevity of antigen-specific T- and B- cell immune memory. Various vaccine and administration factors influence the immune response to vaccination, including the route of vaccine delivery. We studied the dynamics of...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8764446/ https://www.ncbi.nlm.nih.gov/pubmed/35058925 http://dx.doi.org/10.3389/fimmu.2021.784813 |
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author | Feraoun, Yanis Palgen, Jean-Louis Joly, Candie Tchitchek, Nicolas Marcos-Lopez, Ernesto Dereuddre-Bosquet, Nathalie Gallouet, Anne-Sophie Contreras, Vanessa Lévy, Yves Martinon, Frédéric Le Grand, Roger Beignon, Anne-Sophie |
author_facet | Feraoun, Yanis Palgen, Jean-Louis Joly, Candie Tchitchek, Nicolas Marcos-Lopez, Ernesto Dereuddre-Bosquet, Nathalie Gallouet, Anne-Sophie Contreras, Vanessa Lévy, Yves Martinon, Frédéric Le Grand, Roger Beignon, Anne-Sophie |
author_sort | Feraoun, Yanis |
collection | PubMed |
description | Innate immunity modulates adaptive immunity and defines the magnitude, quality, and longevity of antigen-specific T- and B- cell immune memory. Various vaccine and administration factors influence the immune response to vaccination, including the route of vaccine delivery. We studied the dynamics of innate cell responses in blood using a preclinical model of non-human primates immunized with a live attenuated vaccinia virus, a recombinant Modified vaccinia virus Ankara (MVA) expressing a gag-pol-nef fusion of HIV-1, and mass cytometry. We previously showed that it induces a strong, early, and transient innate response, but also late phenotypic modifications of blood myeloid cells after two months when injected subcutaneously. Here, we show that the early innate effector cell responses and plasma inflammatory cytokine profiles differ between subcutaneous and intradermal vaccine injection. Additionally, we show that the intradermal administration fails to induce more highly activated/mature neutrophils long after immunization, in contrast to subcutaneous administration. Different batches of antibodies, staining protocols and generations of mass cytometers were used to generate the two datasets. Mass cytometry data were analyzed in parallel using the same analytical pipeline based on three successive clustering steps, including SPADE, and categorical heatmaps were compared using the Manhattan distance to measure the similarity between cell cluster phenotypes. Overall, we show that the vaccine per se is not sufficient for the late phenotypic modifications of innate myeloid cells, which are evocative of innate immune training. Its route of administration is also crucial, likely by influencing the early innate response, and systemic inflammation, and vaccine biodistribution. |
format | Online Article Text |
id | pubmed-8764446 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-87644462022-01-19 The Route of Vaccine Administration Determines Whether Blood Neutrophils Undergo Long-Term Phenotypic Modifications Feraoun, Yanis Palgen, Jean-Louis Joly, Candie Tchitchek, Nicolas Marcos-Lopez, Ernesto Dereuddre-Bosquet, Nathalie Gallouet, Anne-Sophie Contreras, Vanessa Lévy, Yves Martinon, Frédéric Le Grand, Roger Beignon, Anne-Sophie Front Immunol Immunology Innate immunity modulates adaptive immunity and defines the magnitude, quality, and longevity of antigen-specific T- and B- cell immune memory. Various vaccine and administration factors influence the immune response to vaccination, including the route of vaccine delivery. We studied the dynamics of innate cell responses in blood using a preclinical model of non-human primates immunized with a live attenuated vaccinia virus, a recombinant Modified vaccinia virus Ankara (MVA) expressing a gag-pol-nef fusion of HIV-1, and mass cytometry. We previously showed that it induces a strong, early, and transient innate response, but also late phenotypic modifications of blood myeloid cells after two months when injected subcutaneously. Here, we show that the early innate effector cell responses and plasma inflammatory cytokine profiles differ between subcutaneous and intradermal vaccine injection. Additionally, we show that the intradermal administration fails to induce more highly activated/mature neutrophils long after immunization, in contrast to subcutaneous administration. Different batches of antibodies, staining protocols and generations of mass cytometers were used to generate the two datasets. Mass cytometry data were analyzed in parallel using the same analytical pipeline based on three successive clustering steps, including SPADE, and categorical heatmaps were compared using the Manhattan distance to measure the similarity between cell cluster phenotypes. Overall, we show that the vaccine per se is not sufficient for the late phenotypic modifications of innate myeloid cells, which are evocative of innate immune training. Its route of administration is also crucial, likely by influencing the early innate response, and systemic inflammation, and vaccine biodistribution. Frontiers Media S.A. 2022-01-04 /pmc/articles/PMC8764446/ /pubmed/35058925 http://dx.doi.org/10.3389/fimmu.2021.784813 Text en Copyright © 2022 Feraoun, Palgen, Joly, Tchitchek, Marcos-Lopez, Dereuddre-Bosquet, Gallouet, Contreras, Lévy, Martinon, Le Grand and Beignon https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Feraoun, Yanis Palgen, Jean-Louis Joly, Candie Tchitchek, Nicolas Marcos-Lopez, Ernesto Dereuddre-Bosquet, Nathalie Gallouet, Anne-Sophie Contreras, Vanessa Lévy, Yves Martinon, Frédéric Le Grand, Roger Beignon, Anne-Sophie The Route of Vaccine Administration Determines Whether Blood Neutrophils Undergo Long-Term Phenotypic Modifications |
title | The Route of Vaccine Administration Determines Whether Blood Neutrophils Undergo Long-Term Phenotypic Modifications |
title_full | The Route of Vaccine Administration Determines Whether Blood Neutrophils Undergo Long-Term Phenotypic Modifications |
title_fullStr | The Route of Vaccine Administration Determines Whether Blood Neutrophils Undergo Long-Term Phenotypic Modifications |
title_full_unstemmed | The Route of Vaccine Administration Determines Whether Blood Neutrophils Undergo Long-Term Phenotypic Modifications |
title_short | The Route of Vaccine Administration Determines Whether Blood Neutrophils Undergo Long-Term Phenotypic Modifications |
title_sort | route of vaccine administration determines whether blood neutrophils undergo long-term phenotypic modifications |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8764446/ https://www.ncbi.nlm.nih.gov/pubmed/35058925 http://dx.doi.org/10.3389/fimmu.2021.784813 |
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