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Gram-Negative Bacteria Salmonella typhimurium Boost Leukotriene Synthesis Induced by Chemoattractant fMLP to Stimulate Neutrophil Swarming

Leukotriene synthesis in neutrophils is critical for host survival during infection. In particular, leukotriene B(4) (LTB(4)) is a powerful neutrophil chemoattractant that plays a crucial role in neutrophil swarming. In this work, we demonstrated that preincubation of human neutrophils with Salmonel...

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Autores principales: Golenkina, Ekaterina A., Galkina, Svetlana I., Pletjushkina, Olga, Chernyak, Boris, Gaponova, Tatjana V., Romanova, Yulia M., Sud’ina, Galina F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8764451/
https://www.ncbi.nlm.nih.gov/pubmed/35058789
http://dx.doi.org/10.3389/fphar.2021.814113
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author Golenkina, Ekaterina A.
Galkina, Svetlana I.
Pletjushkina, Olga
Chernyak, Boris
Gaponova, Tatjana V.
Romanova, Yulia M.
Sud’ina, Galina F.
author_facet Golenkina, Ekaterina A.
Galkina, Svetlana I.
Pletjushkina, Olga
Chernyak, Boris
Gaponova, Tatjana V.
Romanova, Yulia M.
Sud’ina, Galina F.
author_sort Golenkina, Ekaterina A.
collection PubMed
description Leukotriene synthesis in neutrophils is critical for host survival during infection. In particular, leukotriene B(4) (LTB(4)) is a powerful neutrophil chemoattractant that plays a crucial role in neutrophil swarming. In this work, we demonstrated that preincubation of human neutrophils with Salmonella typhimurium strongly stimulated LTB(4) production induced by the bacterial chemoattractant, peptide N-formyl-L-methionyl-L-leucyl-l-phenylalanine (fMLP), while the reverse sequence of additions was ineffective. Preincubation with bacterial lipopolysaccharide or yeast polysaccharide zymosan particles gives weaker effect on fMLP-induced LTB(4) production. Activation of 5-lipoxygenase (5-LOX), a key enzyme in leukotrienes biosynthesis, depends on rise of cytosolic concentration of Ca(2+) and on translocation of the enzyme to the nuclear membrane. Both processes were stimulated by S. typhimurium. With an increase in the bacteria:neutrophil ratio, the transformation of LTB(4) to ω-OH-LTB(4) was suppressed, which further supported increased concentration of LTB(4). These data indicate that in neutrophils gathered around bacterial clusters, LTB(4) production is stimulated and at the same time its transformation is suppressed, which promotes neutrophil swarming and elimination of pathogens simultaneously.
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spelling pubmed-87644512022-01-19 Gram-Negative Bacteria Salmonella typhimurium Boost Leukotriene Synthesis Induced by Chemoattractant fMLP to Stimulate Neutrophil Swarming Golenkina, Ekaterina A. Galkina, Svetlana I. Pletjushkina, Olga Chernyak, Boris Gaponova, Tatjana V. Romanova, Yulia M. Sud’ina, Galina F. Front Pharmacol Pharmacology Leukotriene synthesis in neutrophils is critical for host survival during infection. In particular, leukotriene B(4) (LTB(4)) is a powerful neutrophil chemoattractant that plays a crucial role in neutrophil swarming. In this work, we demonstrated that preincubation of human neutrophils with Salmonella typhimurium strongly stimulated LTB(4) production induced by the bacterial chemoattractant, peptide N-formyl-L-methionyl-L-leucyl-l-phenylalanine (fMLP), while the reverse sequence of additions was ineffective. Preincubation with bacterial lipopolysaccharide or yeast polysaccharide zymosan particles gives weaker effect on fMLP-induced LTB(4) production. Activation of 5-lipoxygenase (5-LOX), a key enzyme in leukotrienes biosynthesis, depends on rise of cytosolic concentration of Ca(2+) and on translocation of the enzyme to the nuclear membrane. Both processes were stimulated by S. typhimurium. With an increase in the bacteria:neutrophil ratio, the transformation of LTB(4) to ω-OH-LTB(4) was suppressed, which further supported increased concentration of LTB(4). These data indicate that in neutrophils gathered around bacterial clusters, LTB(4) production is stimulated and at the same time its transformation is suppressed, which promotes neutrophil swarming and elimination of pathogens simultaneously. Frontiers Media S.A. 2022-01-04 /pmc/articles/PMC8764451/ /pubmed/35058789 http://dx.doi.org/10.3389/fphar.2021.814113 Text en Copyright © 2022 Golenkina, Galkina, Pletjushkina, Chernyak, Gaponova, Romanova and Sud’ina. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Golenkina, Ekaterina A.
Galkina, Svetlana I.
Pletjushkina, Olga
Chernyak, Boris
Gaponova, Tatjana V.
Romanova, Yulia M.
Sud’ina, Galina F.
Gram-Negative Bacteria Salmonella typhimurium Boost Leukotriene Synthesis Induced by Chemoattractant fMLP to Stimulate Neutrophil Swarming
title Gram-Negative Bacteria Salmonella typhimurium Boost Leukotriene Synthesis Induced by Chemoattractant fMLP to Stimulate Neutrophil Swarming
title_full Gram-Negative Bacteria Salmonella typhimurium Boost Leukotriene Synthesis Induced by Chemoattractant fMLP to Stimulate Neutrophil Swarming
title_fullStr Gram-Negative Bacteria Salmonella typhimurium Boost Leukotriene Synthesis Induced by Chemoattractant fMLP to Stimulate Neutrophil Swarming
title_full_unstemmed Gram-Negative Bacteria Salmonella typhimurium Boost Leukotriene Synthesis Induced by Chemoattractant fMLP to Stimulate Neutrophil Swarming
title_short Gram-Negative Bacteria Salmonella typhimurium Boost Leukotriene Synthesis Induced by Chemoattractant fMLP to Stimulate Neutrophil Swarming
title_sort gram-negative bacteria salmonella typhimurium boost leukotriene synthesis induced by chemoattractant fmlp to stimulate neutrophil swarming
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8764451/
https://www.ncbi.nlm.nih.gov/pubmed/35058789
http://dx.doi.org/10.3389/fphar.2021.814113
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