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Complete sequences of epidermin and nukacin encoding plasmids from oral-derived Staphylococcus epidermidis and their antibacterial activity

Staphylococcus epidermidis is a commensal bacterium in humans. To persist in the bacterial flora of the host, some bacteria produce antibacterial factors such as the antimicrobial peptides known as bacteriocins. In this study, we tried to isolate bacteriocin-producing S. epidermidis strains. Among 1...

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Autores principales: Nakazono, Kenta, Le, Mi Nguyen-Tra, Kawada-Matsuo, Miki, Kimheang, Noy, Hisatsune, Junzo, Oogai, Yuichi, Nakata, Masanobu, Nakamura, Norifumi, Sugai, Motoyuki, Komatsuzawa, Hitoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8765612/
https://www.ncbi.nlm.nih.gov/pubmed/35041663
http://dx.doi.org/10.1371/journal.pone.0258283
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author Nakazono, Kenta
Le, Mi Nguyen-Tra
Kawada-Matsuo, Miki
Kimheang, Noy
Hisatsune, Junzo
Oogai, Yuichi
Nakata, Masanobu
Nakamura, Norifumi
Sugai, Motoyuki
Komatsuzawa, Hitoshi
author_facet Nakazono, Kenta
Le, Mi Nguyen-Tra
Kawada-Matsuo, Miki
Kimheang, Noy
Hisatsune, Junzo
Oogai, Yuichi
Nakata, Masanobu
Nakamura, Norifumi
Sugai, Motoyuki
Komatsuzawa, Hitoshi
author_sort Nakazono, Kenta
collection PubMed
description Staphylococcus epidermidis is a commensal bacterium in humans. To persist in the bacterial flora of the host, some bacteria produce antibacterial factors such as the antimicrobial peptides known as bacteriocins. In this study, we tried to isolate bacteriocin-producing S. epidermidis strains. Among 150 S. epidermidis isolates from the oral cavities of 287 volunteers, we detected two bacteriocin-producing strains, KSE56 and KSE650. Complete genome sequences of the two strains confirmed that they carried the epidermin-harboring plasmid pEpi56 and the nukacin IVK45-like-harboring plasmid pNuk650. The amino acid sequence of epidermin from KSE56 was identical to the previously reported sequence, but the epidermin synthesis-related genes were partially different. The prepeptide amino acid sequences of nukacin KSE650 and nukacin IVK45 showed one mismatch, but both mature peptides were entirely similar. pNuk650 was larger and had an additional seven ORFs compared to pIVK45. We then investigated the antibacterial activity of the two strains against several skin and oral bacteria and found their different activity patterns. In conclusion, we report the complete sequences of 2 plasmids coding for bacteriocins from S. epidermidis, which were partially different from those previously reported. Furthermore, this is the first report to show the complete sequence of an epidermin-carrying plasmid, pEpi56.
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spelling pubmed-87656122022-01-19 Complete sequences of epidermin and nukacin encoding plasmids from oral-derived Staphylococcus epidermidis and their antibacterial activity Nakazono, Kenta Le, Mi Nguyen-Tra Kawada-Matsuo, Miki Kimheang, Noy Hisatsune, Junzo Oogai, Yuichi Nakata, Masanobu Nakamura, Norifumi Sugai, Motoyuki Komatsuzawa, Hitoshi PLoS One Research Article Staphylococcus epidermidis is a commensal bacterium in humans. To persist in the bacterial flora of the host, some bacteria produce antibacterial factors such as the antimicrobial peptides known as bacteriocins. In this study, we tried to isolate bacteriocin-producing S. epidermidis strains. Among 150 S. epidermidis isolates from the oral cavities of 287 volunteers, we detected two bacteriocin-producing strains, KSE56 and KSE650. Complete genome sequences of the two strains confirmed that they carried the epidermin-harboring plasmid pEpi56 and the nukacin IVK45-like-harboring plasmid pNuk650. The amino acid sequence of epidermin from KSE56 was identical to the previously reported sequence, but the epidermin synthesis-related genes were partially different. The prepeptide amino acid sequences of nukacin KSE650 and nukacin IVK45 showed one mismatch, but both mature peptides were entirely similar. pNuk650 was larger and had an additional seven ORFs compared to pIVK45. We then investigated the antibacterial activity of the two strains against several skin and oral bacteria and found their different activity patterns. In conclusion, we report the complete sequences of 2 plasmids coding for bacteriocins from S. epidermidis, which were partially different from those previously reported. Furthermore, this is the first report to show the complete sequence of an epidermin-carrying plasmid, pEpi56. Public Library of Science 2022-01-18 /pmc/articles/PMC8765612/ /pubmed/35041663 http://dx.doi.org/10.1371/journal.pone.0258283 Text en © 2022 Nakazono et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Nakazono, Kenta
Le, Mi Nguyen-Tra
Kawada-Matsuo, Miki
Kimheang, Noy
Hisatsune, Junzo
Oogai, Yuichi
Nakata, Masanobu
Nakamura, Norifumi
Sugai, Motoyuki
Komatsuzawa, Hitoshi
Complete sequences of epidermin and nukacin encoding plasmids from oral-derived Staphylococcus epidermidis and their antibacterial activity
title Complete sequences of epidermin and nukacin encoding plasmids from oral-derived Staphylococcus epidermidis and their antibacterial activity
title_full Complete sequences of epidermin and nukacin encoding plasmids from oral-derived Staphylococcus epidermidis and their antibacterial activity
title_fullStr Complete sequences of epidermin and nukacin encoding plasmids from oral-derived Staphylococcus epidermidis and their antibacterial activity
title_full_unstemmed Complete sequences of epidermin and nukacin encoding plasmids from oral-derived Staphylococcus epidermidis and their antibacterial activity
title_short Complete sequences of epidermin and nukacin encoding plasmids from oral-derived Staphylococcus epidermidis and their antibacterial activity
title_sort complete sequences of epidermin and nukacin encoding plasmids from oral-derived staphylococcus epidermidis and their antibacterial activity
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8765612/
https://www.ncbi.nlm.nih.gov/pubmed/35041663
http://dx.doi.org/10.1371/journal.pone.0258283
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