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Preformation and epigenesis converge to specify primordial germ cell fate in the early Drosophila embryo

A critical step in animal development is the specification of primordial germ cells (PGCs), the precursors of the germline. Two seemingly mutually exclusive mechanisms are implemented across the animal kingdom: epigenesis and preformation. In epigenesis, PGC specification is non-autonomous and depen...

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Autores principales: Colonnetta, Megan M., Goyal, Yogesh, Johnson, Heath E., Syal, Sapna, Schedl, Paul, Deshpande, Girish
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8765614/
https://www.ncbi.nlm.nih.gov/pubmed/34986144
http://dx.doi.org/10.1371/journal.pgen.1010002
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author Colonnetta, Megan M.
Goyal, Yogesh
Johnson, Heath E.
Syal, Sapna
Schedl, Paul
Deshpande, Girish
author_facet Colonnetta, Megan M.
Goyal, Yogesh
Johnson, Heath E.
Syal, Sapna
Schedl, Paul
Deshpande, Girish
author_sort Colonnetta, Megan M.
collection PubMed
description A critical step in animal development is the specification of primordial germ cells (PGCs), the precursors of the germline. Two seemingly mutually exclusive mechanisms are implemented across the animal kingdom: epigenesis and preformation. In epigenesis, PGC specification is non-autonomous and depends on extrinsic signaling pathways. The BMP pathway provides the key PGC specification signals in mammals. Preformation is autonomous and mediated by determinants localized within PGCs. In Drosophila, a classic example of preformation, constituents of the germ plasm localized at the embryonic posterior are thought to be both necessary and sufficient for proper determination of PGCs. Contrary to this longstanding model, here we show that these localized determinants are insufficient by themselves to direct PGC specification in blastoderm stage embryos. Instead, we find that the BMP signaling pathway is required at multiple steps during the specification process and functions in conjunction with components of the germ plasm to orchestrate PGC fate.
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spelling pubmed-87656142022-01-19 Preformation and epigenesis converge to specify primordial germ cell fate in the early Drosophila embryo Colonnetta, Megan M. Goyal, Yogesh Johnson, Heath E. Syal, Sapna Schedl, Paul Deshpande, Girish PLoS Genet Research Article A critical step in animal development is the specification of primordial germ cells (PGCs), the precursors of the germline. Two seemingly mutually exclusive mechanisms are implemented across the animal kingdom: epigenesis and preformation. In epigenesis, PGC specification is non-autonomous and depends on extrinsic signaling pathways. The BMP pathway provides the key PGC specification signals in mammals. Preformation is autonomous and mediated by determinants localized within PGCs. In Drosophila, a classic example of preformation, constituents of the germ plasm localized at the embryonic posterior are thought to be both necessary and sufficient for proper determination of PGCs. Contrary to this longstanding model, here we show that these localized determinants are insufficient by themselves to direct PGC specification in blastoderm stage embryos. Instead, we find that the BMP signaling pathway is required at multiple steps during the specification process and functions in conjunction with components of the germ plasm to orchestrate PGC fate. Public Library of Science 2022-01-05 /pmc/articles/PMC8765614/ /pubmed/34986144 http://dx.doi.org/10.1371/journal.pgen.1010002 Text en © 2022 Colonnetta et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Colonnetta, Megan M.
Goyal, Yogesh
Johnson, Heath E.
Syal, Sapna
Schedl, Paul
Deshpande, Girish
Preformation and epigenesis converge to specify primordial germ cell fate in the early Drosophila embryo
title Preformation and epigenesis converge to specify primordial germ cell fate in the early Drosophila embryo
title_full Preformation and epigenesis converge to specify primordial germ cell fate in the early Drosophila embryo
title_fullStr Preformation and epigenesis converge to specify primordial germ cell fate in the early Drosophila embryo
title_full_unstemmed Preformation and epigenesis converge to specify primordial germ cell fate in the early Drosophila embryo
title_short Preformation and epigenesis converge to specify primordial germ cell fate in the early Drosophila embryo
title_sort preformation and epigenesis converge to specify primordial germ cell fate in the early drosophila embryo
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8765614/
https://www.ncbi.nlm.nih.gov/pubmed/34986144
http://dx.doi.org/10.1371/journal.pgen.1010002
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