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Toward Multiplexed Optogenetic Circuits

Owing to its ubiquity and easy availability in nature, light has been widely employed to control complex cellular behaviors. Light-sensitive proteins are the foundation to such diverse and multilevel adaptive regulations in a large range of organisms. Due to their remarkable properties and potential...

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Autores principales: Dwijayanti, Ari, Zhang, Congqiang, Poh, Chueh Loo, Lautier, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8766309/
https://www.ncbi.nlm.nih.gov/pubmed/35071213
http://dx.doi.org/10.3389/fbioe.2021.804563
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author Dwijayanti, Ari
Zhang, Congqiang
Poh, Chueh Loo
Lautier, Thomas
author_facet Dwijayanti, Ari
Zhang, Congqiang
Poh, Chueh Loo
Lautier, Thomas
author_sort Dwijayanti, Ari
collection PubMed
description Owing to its ubiquity and easy availability in nature, light has been widely employed to control complex cellular behaviors. Light-sensitive proteins are the foundation to such diverse and multilevel adaptive regulations in a large range of organisms. Due to their remarkable properties and potential applications in engineered systems, exploration and engineering of natural light-sensitive proteins have significantly contributed to expand optogenetic toolboxes with tailor-made performances in synthetic genetic circuits. Progressively, more complex systems have been designed in which multiple photoreceptors, each sensing its dedicated wavelength, are combined to simultaneously coordinate cellular responses in a single cell. In this review, we highlight recent works and challenges on multiplexed optogenetic circuits in natural and engineered systems for a dynamic regulation breakthrough in biotechnological applications.
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spelling pubmed-87663092022-01-20 Toward Multiplexed Optogenetic Circuits Dwijayanti, Ari Zhang, Congqiang Poh, Chueh Loo Lautier, Thomas Front Bioeng Biotechnol Bioengineering and Biotechnology Owing to its ubiquity and easy availability in nature, light has been widely employed to control complex cellular behaviors. Light-sensitive proteins are the foundation to such diverse and multilevel adaptive regulations in a large range of organisms. Due to their remarkable properties and potential applications in engineered systems, exploration and engineering of natural light-sensitive proteins have significantly contributed to expand optogenetic toolboxes with tailor-made performances in synthetic genetic circuits. Progressively, more complex systems have been designed in which multiple photoreceptors, each sensing its dedicated wavelength, are combined to simultaneously coordinate cellular responses in a single cell. In this review, we highlight recent works and challenges on multiplexed optogenetic circuits in natural and engineered systems for a dynamic regulation breakthrough in biotechnological applications. Frontiers Media S.A. 2022-01-05 /pmc/articles/PMC8766309/ /pubmed/35071213 http://dx.doi.org/10.3389/fbioe.2021.804563 Text en Copyright © 2022 Dwijayanti, Zhang, Poh and Lautier. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Dwijayanti, Ari
Zhang, Congqiang
Poh, Chueh Loo
Lautier, Thomas
Toward Multiplexed Optogenetic Circuits
title Toward Multiplexed Optogenetic Circuits
title_full Toward Multiplexed Optogenetic Circuits
title_fullStr Toward Multiplexed Optogenetic Circuits
title_full_unstemmed Toward Multiplexed Optogenetic Circuits
title_short Toward Multiplexed Optogenetic Circuits
title_sort toward multiplexed optogenetic circuits
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8766309/
https://www.ncbi.nlm.nih.gov/pubmed/35071213
http://dx.doi.org/10.3389/fbioe.2021.804563
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