Cargando…
Toward Multiplexed Optogenetic Circuits
Owing to its ubiquity and easy availability in nature, light has been widely employed to control complex cellular behaviors. Light-sensitive proteins are the foundation to such diverse and multilevel adaptive regulations in a large range of organisms. Due to their remarkable properties and potential...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8766309/ https://www.ncbi.nlm.nih.gov/pubmed/35071213 http://dx.doi.org/10.3389/fbioe.2021.804563 |
_version_ | 1784634501832376320 |
---|---|
author | Dwijayanti, Ari Zhang, Congqiang Poh, Chueh Loo Lautier, Thomas |
author_facet | Dwijayanti, Ari Zhang, Congqiang Poh, Chueh Loo Lautier, Thomas |
author_sort | Dwijayanti, Ari |
collection | PubMed |
description | Owing to its ubiquity and easy availability in nature, light has been widely employed to control complex cellular behaviors. Light-sensitive proteins are the foundation to such diverse and multilevel adaptive regulations in a large range of organisms. Due to their remarkable properties and potential applications in engineered systems, exploration and engineering of natural light-sensitive proteins have significantly contributed to expand optogenetic toolboxes with tailor-made performances in synthetic genetic circuits. Progressively, more complex systems have been designed in which multiple photoreceptors, each sensing its dedicated wavelength, are combined to simultaneously coordinate cellular responses in a single cell. In this review, we highlight recent works and challenges on multiplexed optogenetic circuits in natural and engineered systems for a dynamic regulation breakthrough in biotechnological applications. |
format | Online Article Text |
id | pubmed-8766309 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-87663092022-01-20 Toward Multiplexed Optogenetic Circuits Dwijayanti, Ari Zhang, Congqiang Poh, Chueh Loo Lautier, Thomas Front Bioeng Biotechnol Bioengineering and Biotechnology Owing to its ubiquity and easy availability in nature, light has been widely employed to control complex cellular behaviors. Light-sensitive proteins are the foundation to such diverse and multilevel adaptive regulations in a large range of organisms. Due to their remarkable properties and potential applications in engineered systems, exploration and engineering of natural light-sensitive proteins have significantly contributed to expand optogenetic toolboxes with tailor-made performances in synthetic genetic circuits. Progressively, more complex systems have been designed in which multiple photoreceptors, each sensing its dedicated wavelength, are combined to simultaneously coordinate cellular responses in a single cell. In this review, we highlight recent works and challenges on multiplexed optogenetic circuits in natural and engineered systems for a dynamic regulation breakthrough in biotechnological applications. Frontiers Media S.A. 2022-01-05 /pmc/articles/PMC8766309/ /pubmed/35071213 http://dx.doi.org/10.3389/fbioe.2021.804563 Text en Copyright © 2022 Dwijayanti, Zhang, Poh and Lautier. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Dwijayanti, Ari Zhang, Congqiang Poh, Chueh Loo Lautier, Thomas Toward Multiplexed Optogenetic Circuits |
title | Toward Multiplexed Optogenetic Circuits |
title_full | Toward Multiplexed Optogenetic Circuits |
title_fullStr | Toward Multiplexed Optogenetic Circuits |
title_full_unstemmed | Toward Multiplexed Optogenetic Circuits |
title_short | Toward Multiplexed Optogenetic Circuits |
title_sort | toward multiplexed optogenetic circuits |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8766309/ https://www.ncbi.nlm.nih.gov/pubmed/35071213 http://dx.doi.org/10.3389/fbioe.2021.804563 |
work_keys_str_mv | AT dwijayantiari towardmultiplexedoptogeneticcircuits AT zhangcongqiang towardmultiplexedoptogeneticcircuits AT pohchuehloo towardmultiplexedoptogeneticcircuits AT lautierthomas towardmultiplexedoptogeneticcircuits |