Cargando…

Building a Barrier: The Influence of Different Wax Fractions on the Water Transpiration Barrier of Leaf Cuticles

Waxes are critical in limiting non-stomatal water loss in higher terrestrial plants by making up the limiting barrier for water diffusion across cuticles. Using a differential extraction protocol, we investigated the influence of various wax fractions on the cuticular transpiration barrier. Triterpe...

Descripción completa

Detalles Bibliográficos
Autores principales: Seufert, Pascal, Staiger, Simona, Arand, Katja, Bueno, Amauri, Burghardt, Markus, Riederer, Markus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8766326/
https://www.ncbi.nlm.nih.gov/pubmed/35069622
http://dx.doi.org/10.3389/fpls.2021.766602
_version_ 1784634504427601920
author Seufert, Pascal
Staiger, Simona
Arand, Katja
Bueno, Amauri
Burghardt, Markus
Riederer, Markus
author_facet Seufert, Pascal
Staiger, Simona
Arand, Katja
Bueno, Amauri
Burghardt, Markus
Riederer, Markus
author_sort Seufert, Pascal
collection PubMed
description Waxes are critical in limiting non-stomatal water loss in higher terrestrial plants by making up the limiting barrier for water diffusion across cuticles. Using a differential extraction protocol, we investigated the influence of various wax fractions on the cuticular transpiration barrier. Triterpenoids (TRPs) and very long-chain aliphatics (VLCAs) were selectively extracted from isolated adaxial leaf cuticles using methanol (MeOH) followed by chloroform (TCM). The water permeabilities of the native and the solvent-treated cuticles were measured gravimetrically. Seven plant species (Camellia sinensis, Ficus elastica, Hedera helix, Ilex aquifolium, Nerium oleander, Vinca minor, and Zamioculcas zamiifolia) with highly varying wax compositions ranging from nearly pure VLCA- to TRP-dominated waxes were selected. After TRP removal with MeOH, water permeability did not or only slightly increase. The subsequent VLCA extraction with TCM led to increases in cuticular water permeabilities by up to two orders of magnitude. These effects were consistent across all species investigated, providing direct evidence that the cuticular transpiration barrier is mainly composed of VLCA. In contrast, TRPs play no or only a minor role in controlling water loss.
format Online
Article
Text
id pubmed-8766326
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-87663262022-01-20 Building a Barrier: The Influence of Different Wax Fractions on the Water Transpiration Barrier of Leaf Cuticles Seufert, Pascal Staiger, Simona Arand, Katja Bueno, Amauri Burghardt, Markus Riederer, Markus Front Plant Sci Plant Science Waxes are critical in limiting non-stomatal water loss in higher terrestrial plants by making up the limiting barrier for water diffusion across cuticles. Using a differential extraction protocol, we investigated the influence of various wax fractions on the cuticular transpiration barrier. Triterpenoids (TRPs) and very long-chain aliphatics (VLCAs) were selectively extracted from isolated adaxial leaf cuticles using methanol (MeOH) followed by chloroform (TCM). The water permeabilities of the native and the solvent-treated cuticles were measured gravimetrically. Seven plant species (Camellia sinensis, Ficus elastica, Hedera helix, Ilex aquifolium, Nerium oleander, Vinca minor, and Zamioculcas zamiifolia) with highly varying wax compositions ranging from nearly pure VLCA- to TRP-dominated waxes were selected. After TRP removal with MeOH, water permeability did not or only slightly increase. The subsequent VLCA extraction with TCM led to increases in cuticular water permeabilities by up to two orders of magnitude. These effects were consistent across all species investigated, providing direct evidence that the cuticular transpiration barrier is mainly composed of VLCA. In contrast, TRPs play no or only a minor role in controlling water loss. Frontiers Media S.A. 2022-01-05 /pmc/articles/PMC8766326/ /pubmed/35069622 http://dx.doi.org/10.3389/fpls.2021.766602 Text en Copyright © 2022 Seufert, Staiger, Arand, Bueno, Burghardt and Riederer. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Seufert, Pascal
Staiger, Simona
Arand, Katja
Bueno, Amauri
Burghardt, Markus
Riederer, Markus
Building a Barrier: The Influence of Different Wax Fractions on the Water Transpiration Barrier of Leaf Cuticles
title Building a Barrier: The Influence of Different Wax Fractions on the Water Transpiration Barrier of Leaf Cuticles
title_full Building a Barrier: The Influence of Different Wax Fractions on the Water Transpiration Barrier of Leaf Cuticles
title_fullStr Building a Barrier: The Influence of Different Wax Fractions on the Water Transpiration Barrier of Leaf Cuticles
title_full_unstemmed Building a Barrier: The Influence of Different Wax Fractions on the Water Transpiration Barrier of Leaf Cuticles
title_short Building a Barrier: The Influence of Different Wax Fractions on the Water Transpiration Barrier of Leaf Cuticles
title_sort building a barrier: the influence of different wax fractions on the water transpiration barrier of leaf cuticles
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8766326/
https://www.ncbi.nlm.nih.gov/pubmed/35069622
http://dx.doi.org/10.3389/fpls.2021.766602
work_keys_str_mv AT seufertpascal buildingabarriertheinfluenceofdifferentwaxfractionsonthewatertranspirationbarrierofleafcuticles
AT staigersimona buildingabarriertheinfluenceofdifferentwaxfractionsonthewatertranspirationbarrierofleafcuticles
AT arandkatja buildingabarriertheinfluenceofdifferentwaxfractionsonthewatertranspirationbarrierofleafcuticles
AT buenoamauri buildingabarriertheinfluenceofdifferentwaxfractionsonthewatertranspirationbarrierofleafcuticles
AT burghardtmarkus buildingabarriertheinfluenceofdifferentwaxfractionsonthewatertranspirationbarrierofleafcuticles
AT riederermarkus buildingabarriertheinfluenceofdifferentwaxfractionsonthewatertranspirationbarrierofleafcuticles