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Macrophage-Derived Exosomes Promote Bone Mesenchymal Stem Cells Towards Osteoblastic Fate Through microRNA-21a-5p

The effective healing of a bone defect is dependent on the careful coordination of inflammatory and bone-forming cells. In the current work, pro-inflammatory M1 and anti-inflammatory M2 macrophages were co-cultured with primary murine bone mesenchymal stem cells (BMSCs), in vitro, to establish the c...

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Autores principales: Liu, Kun, Luo, Xin, Lv, Zhao-Yong, Zhang, Yu-Jue, Meng, Zhen, Li, Jun, Meng, Chun-Xiu, Qiang, Hui-Fen, Hou, Cai-Yao, Hou, Lei, Liu, Feng-Zhen, Zhang, Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8766741/
https://www.ncbi.nlm.nih.gov/pubmed/35071209
http://dx.doi.org/10.3389/fbioe.2021.801432
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author Liu, Kun
Luo, Xin
Lv, Zhao-Yong
Zhang, Yu-Jue
Meng, Zhen
Li, Jun
Meng, Chun-Xiu
Qiang, Hui-Fen
Hou, Cai-Yao
Hou, Lei
Liu, Feng-Zhen
Zhang, Bin
author_facet Liu, Kun
Luo, Xin
Lv, Zhao-Yong
Zhang, Yu-Jue
Meng, Zhen
Li, Jun
Meng, Chun-Xiu
Qiang, Hui-Fen
Hou, Cai-Yao
Hou, Lei
Liu, Feng-Zhen
Zhang, Bin
author_sort Liu, Kun
collection PubMed
description The effective healing of a bone defect is dependent on the careful coordination of inflammatory and bone-forming cells. In the current work, pro-inflammatory M1 and anti-inflammatory M2 macrophages were co-cultured with primary murine bone mesenchymal stem cells (BMSCs), in vitro, to establish the cross-talk among polarized macrophages and BMSCs, and as well as their effects on osteogenesis. Meanwhile, macrophages influence the osteogenesis of BMSCs through paracrine forms such as exosomes. We focused on whether exosomes of macrophages promote osteogenic differentiation. The results indicated that M1 and M2 polarized macrophage exosomes all can promote osteogenesis of BMSCs. Especially, M1 macrophage-derived exosomes promote osteogenesis of BMSCs through microRNA-21a-5p at the early stage of inflammation. This research helps to develop an understanding of the intricate interactions among BMSCs and macrophages, which can help to improve the process of bone healing as well as additional regenerative processes by local sustained release of exosomes.
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spelling pubmed-87667412022-01-20 Macrophage-Derived Exosomes Promote Bone Mesenchymal Stem Cells Towards Osteoblastic Fate Through microRNA-21a-5p Liu, Kun Luo, Xin Lv, Zhao-Yong Zhang, Yu-Jue Meng, Zhen Li, Jun Meng, Chun-Xiu Qiang, Hui-Fen Hou, Cai-Yao Hou, Lei Liu, Feng-Zhen Zhang, Bin Front Bioeng Biotechnol Bioengineering and Biotechnology The effective healing of a bone defect is dependent on the careful coordination of inflammatory and bone-forming cells. In the current work, pro-inflammatory M1 and anti-inflammatory M2 macrophages were co-cultured with primary murine bone mesenchymal stem cells (BMSCs), in vitro, to establish the cross-talk among polarized macrophages and BMSCs, and as well as their effects on osteogenesis. Meanwhile, macrophages influence the osteogenesis of BMSCs through paracrine forms such as exosomes. We focused on whether exosomes of macrophages promote osteogenic differentiation. The results indicated that M1 and M2 polarized macrophage exosomes all can promote osteogenesis of BMSCs. Especially, M1 macrophage-derived exosomes promote osteogenesis of BMSCs through microRNA-21a-5p at the early stage of inflammation. This research helps to develop an understanding of the intricate interactions among BMSCs and macrophages, which can help to improve the process of bone healing as well as additional regenerative processes by local sustained release of exosomes. Frontiers Media S.A. 2022-01-05 /pmc/articles/PMC8766741/ /pubmed/35071209 http://dx.doi.org/10.3389/fbioe.2021.801432 Text en Copyright © 2022 Liu, Luo, Lv, Zhang, Meng, Li, Meng, Qiang, Hou, Hou, Liu and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Liu, Kun
Luo, Xin
Lv, Zhao-Yong
Zhang, Yu-Jue
Meng, Zhen
Li, Jun
Meng, Chun-Xiu
Qiang, Hui-Fen
Hou, Cai-Yao
Hou, Lei
Liu, Feng-Zhen
Zhang, Bin
Macrophage-Derived Exosomes Promote Bone Mesenchymal Stem Cells Towards Osteoblastic Fate Through microRNA-21a-5p
title Macrophage-Derived Exosomes Promote Bone Mesenchymal Stem Cells Towards Osteoblastic Fate Through microRNA-21a-5p
title_full Macrophage-Derived Exosomes Promote Bone Mesenchymal Stem Cells Towards Osteoblastic Fate Through microRNA-21a-5p
title_fullStr Macrophage-Derived Exosomes Promote Bone Mesenchymal Stem Cells Towards Osteoblastic Fate Through microRNA-21a-5p
title_full_unstemmed Macrophage-Derived Exosomes Promote Bone Mesenchymal Stem Cells Towards Osteoblastic Fate Through microRNA-21a-5p
title_short Macrophage-Derived Exosomes Promote Bone Mesenchymal Stem Cells Towards Osteoblastic Fate Through microRNA-21a-5p
title_sort macrophage-derived exosomes promote bone mesenchymal stem cells towards osteoblastic fate through microrna-21a-5p
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8766741/
https://www.ncbi.nlm.nih.gov/pubmed/35071209
http://dx.doi.org/10.3389/fbioe.2021.801432
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