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Induction of Functional Specific Antibodies, IgG-Secreting Plasmablasts and Memory B Cells Following BCG Vaccination
Tuberculosis (TB) is a major global health problem and the only currently-licensed vaccine, BCG, is inadequate. Many TB vaccine candidates are designed to be given as a boost to BCG; an understanding of the BCG-induced immune response is therefore critical, and the opportunity to relate this to circ...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8767055/ https://www.ncbi.nlm.nih.gov/pubmed/35069580 http://dx.doi.org/10.3389/fimmu.2021.798207 |
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author | Bitencourt, Julia Peralta-Álvarez, Marco Polo Wilkie, Morven Jacobs, Ashley Wright, Daniel Salman Almujri, Salem Li, Shuailin Harris, Stephanie A. Smith, Steven G. Elias, Sean C. White, Andrew D. Satti, Iman Sharpe, Sally S. O’Shea, Matthew K. McShane, Helen Tanner, Rachel |
author_facet | Bitencourt, Julia Peralta-Álvarez, Marco Polo Wilkie, Morven Jacobs, Ashley Wright, Daniel Salman Almujri, Salem Li, Shuailin Harris, Stephanie A. Smith, Steven G. Elias, Sean C. White, Andrew D. Satti, Iman Sharpe, Sally S. O’Shea, Matthew K. McShane, Helen Tanner, Rachel |
author_sort | Bitencourt, Julia |
collection | PubMed |
description | Tuberculosis (TB) is a major global health problem and the only currently-licensed vaccine, BCG, is inadequate. Many TB vaccine candidates are designed to be given as a boost to BCG; an understanding of the BCG-induced immune response is therefore critical, and the opportunity to relate this to circumstances where BCG does confer protection may direct the design of more efficacious vaccines. While the T cell response to BCG vaccination has been well-characterized, there is a paucity of literature on the humoral response. We demonstrate BCG vaccine-mediated induction of specific antibodies in different human populations and macaque species which represent important preclinical models for TB vaccine development. We observe a strong correlation between antibody titers in serum versus plasma with modestly higher titers in serum. We also report for the first time the rapid and transient induction of antibody-secreting plasmablasts following BCG vaccination, together with a robust and durable memory B cell response in humans. Finally, we demonstrate a functional role for BCG vaccine-induced specific antibodies in opsonizing mycobacteria and enhancing macrophage phagocytosis in vitro, which may contribute to the BCG vaccine-mediated control of mycobacterial growth observed. Taken together, our findings indicate that the humoral immune response in the context of BCG vaccination merits further attention to determine whether TB vaccine candidates could benefit from the induction of humoral as well as cellular immunity. |
format | Online Article Text |
id | pubmed-8767055 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-87670552022-01-20 Induction of Functional Specific Antibodies, IgG-Secreting Plasmablasts and Memory B Cells Following BCG Vaccination Bitencourt, Julia Peralta-Álvarez, Marco Polo Wilkie, Morven Jacobs, Ashley Wright, Daniel Salman Almujri, Salem Li, Shuailin Harris, Stephanie A. Smith, Steven G. Elias, Sean C. White, Andrew D. Satti, Iman Sharpe, Sally S. O’Shea, Matthew K. McShane, Helen Tanner, Rachel Front Immunol Immunology Tuberculosis (TB) is a major global health problem and the only currently-licensed vaccine, BCG, is inadequate. Many TB vaccine candidates are designed to be given as a boost to BCG; an understanding of the BCG-induced immune response is therefore critical, and the opportunity to relate this to circumstances where BCG does confer protection may direct the design of more efficacious vaccines. While the T cell response to BCG vaccination has been well-characterized, there is a paucity of literature on the humoral response. We demonstrate BCG vaccine-mediated induction of specific antibodies in different human populations and macaque species which represent important preclinical models for TB vaccine development. We observe a strong correlation between antibody titers in serum versus plasma with modestly higher titers in serum. We also report for the first time the rapid and transient induction of antibody-secreting plasmablasts following BCG vaccination, together with a robust and durable memory B cell response in humans. Finally, we demonstrate a functional role for BCG vaccine-induced specific antibodies in opsonizing mycobacteria and enhancing macrophage phagocytosis in vitro, which may contribute to the BCG vaccine-mediated control of mycobacterial growth observed. Taken together, our findings indicate that the humoral immune response in the context of BCG vaccination merits further attention to determine whether TB vaccine candidates could benefit from the induction of humoral as well as cellular immunity. Frontiers Media S.A. 2022-01-05 /pmc/articles/PMC8767055/ /pubmed/35069580 http://dx.doi.org/10.3389/fimmu.2021.798207 Text en Copyright © 2022 Bitencourt, Peralta-Álvarez, Wilkie, Jacobs, Wright, Salman Almujri, Li, Harris, Smith, Elias, White, Satti, Sharpe, O’Shea, McShane and Tanner https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Bitencourt, Julia Peralta-Álvarez, Marco Polo Wilkie, Morven Jacobs, Ashley Wright, Daniel Salman Almujri, Salem Li, Shuailin Harris, Stephanie A. Smith, Steven G. Elias, Sean C. White, Andrew D. Satti, Iman Sharpe, Sally S. O’Shea, Matthew K. McShane, Helen Tanner, Rachel Induction of Functional Specific Antibodies, IgG-Secreting Plasmablasts and Memory B Cells Following BCG Vaccination |
title | Induction of Functional Specific Antibodies, IgG-Secreting Plasmablasts and Memory B Cells Following BCG Vaccination |
title_full | Induction of Functional Specific Antibodies, IgG-Secreting Plasmablasts and Memory B Cells Following BCG Vaccination |
title_fullStr | Induction of Functional Specific Antibodies, IgG-Secreting Plasmablasts and Memory B Cells Following BCG Vaccination |
title_full_unstemmed | Induction of Functional Specific Antibodies, IgG-Secreting Plasmablasts and Memory B Cells Following BCG Vaccination |
title_short | Induction of Functional Specific Antibodies, IgG-Secreting Plasmablasts and Memory B Cells Following BCG Vaccination |
title_sort | induction of functional specific antibodies, igg-secreting plasmablasts and memory b cells following bcg vaccination |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8767055/ https://www.ncbi.nlm.nih.gov/pubmed/35069580 http://dx.doi.org/10.3389/fimmu.2021.798207 |
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