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Intra-Species Interactions in Streptococcus pneumoniae Biofilms

Streptococcus pneumoniae is a human pathogen responsible for high morbidity and mortality worldwide. Disease is incidental and is preceded by asymptomatic nasopharyngeal colonization in the form of biofilms. Simultaneous colonization by multiple pneumococcal strains is frequent but remains poorly ch...

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Autores principales: Valente, Carina, Cruz, Ana R., Henriques, Adriano O., Sá-Leão, Raquel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8767070/
https://www.ncbi.nlm.nih.gov/pubmed/35071049
http://dx.doi.org/10.3389/fcimb.2021.803286
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author Valente, Carina
Cruz, Ana R.
Henriques, Adriano O.
Sá-Leão, Raquel
author_facet Valente, Carina
Cruz, Ana R.
Henriques, Adriano O.
Sá-Leão, Raquel
author_sort Valente, Carina
collection PubMed
description Streptococcus pneumoniae is a human pathogen responsible for high morbidity and mortality worldwide. Disease is incidental and is preceded by asymptomatic nasopharyngeal colonization in the form of biofilms. Simultaneous colonization by multiple pneumococcal strains is frequent but remains poorly characterized. Previous studies, using mostly laboratory strains, showed that pneumococcal strains can reciprocally affect each other’s colonization ability. Here, we aimed at developing a strategy to investigate pneumococcal intra-species interactions occurring in biofilms. A 72h abiotic biofilm model mimicking long-term colonization was applied to study eight pneumococcal strains encompassing 6 capsular types and 7 multilocus sequence types. Strains were labeled with GFP or RFP, generating two fluorescent variants for each. Intra-species interactions were evaluated in dual-strain biofilms (1:1 ratio) using flow cytometry. Confocal microscopy was used to image representative biofilms. Twenty-eight dual-strain combinations were tested. Interactions of commensalism, competition, amensalism and neutralism were identified. The outcome of an interaction was independent of the capsular and sequence type of the strains involved. Confocal imaging of biofilms confirmed the positive, negative and neutral effects that pneumococci can exert on each other. In conclusion, we developed an experimental approach that successfully discriminates pneumococcal strains growing in mixed biofilms, which enables the identification of intra-species interactions. Several types of interactions occur among pneumococci. These observations are a starting point to study the mechanisms underlying those interactions.
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spelling pubmed-87670702022-01-20 Intra-Species Interactions in Streptococcus pneumoniae Biofilms Valente, Carina Cruz, Ana R. Henriques, Adriano O. Sá-Leão, Raquel Front Cell Infect Microbiol Cellular and Infection Microbiology Streptococcus pneumoniae is a human pathogen responsible for high morbidity and mortality worldwide. Disease is incidental and is preceded by asymptomatic nasopharyngeal colonization in the form of biofilms. Simultaneous colonization by multiple pneumococcal strains is frequent but remains poorly characterized. Previous studies, using mostly laboratory strains, showed that pneumococcal strains can reciprocally affect each other’s colonization ability. Here, we aimed at developing a strategy to investigate pneumococcal intra-species interactions occurring in biofilms. A 72h abiotic biofilm model mimicking long-term colonization was applied to study eight pneumococcal strains encompassing 6 capsular types and 7 multilocus sequence types. Strains were labeled with GFP or RFP, generating two fluorescent variants for each. Intra-species interactions were evaluated in dual-strain biofilms (1:1 ratio) using flow cytometry. Confocal microscopy was used to image representative biofilms. Twenty-eight dual-strain combinations were tested. Interactions of commensalism, competition, amensalism and neutralism were identified. The outcome of an interaction was independent of the capsular and sequence type of the strains involved. Confocal imaging of biofilms confirmed the positive, negative and neutral effects that pneumococci can exert on each other. In conclusion, we developed an experimental approach that successfully discriminates pneumococcal strains growing in mixed biofilms, which enables the identification of intra-species interactions. Several types of interactions occur among pneumococci. These observations are a starting point to study the mechanisms underlying those interactions. Frontiers Media S.A. 2022-01-05 /pmc/articles/PMC8767070/ /pubmed/35071049 http://dx.doi.org/10.3389/fcimb.2021.803286 Text en Copyright © 2022 Valente, Cruz, Henriques and Sá-Leão https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Valente, Carina
Cruz, Ana R.
Henriques, Adriano O.
Sá-Leão, Raquel
Intra-Species Interactions in Streptococcus pneumoniae Biofilms
title Intra-Species Interactions in Streptococcus pneumoniae Biofilms
title_full Intra-Species Interactions in Streptococcus pneumoniae Biofilms
title_fullStr Intra-Species Interactions in Streptococcus pneumoniae Biofilms
title_full_unstemmed Intra-Species Interactions in Streptococcus pneumoniae Biofilms
title_short Intra-Species Interactions in Streptococcus pneumoniae Biofilms
title_sort intra-species interactions in streptococcus pneumoniae biofilms
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8767070/
https://www.ncbi.nlm.nih.gov/pubmed/35071049
http://dx.doi.org/10.3389/fcimb.2021.803286
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