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Genome-wide analysis of fitness-factors in uropathogenic Escherichia coli during growth in laboratory media and during urinary tract infections

Uropathogenic Escherichia coli (UPEC) UTI89 is a well-characterized strain, which has mainly been used to study UPEC virulence during urinary tract infection (UTI). However, little is known on UTI89 key fitness-factors during growth in lab media and during UTI. Here, we used a transposon-insertion-s...

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Autores principales: García, Vanesa, Grønnemose, Rasmus B., Torres-Puig, Sergi, Kudirkiene, Egle, Piantelli, Mateo, Ahmed, Shahana, Andersen, Thomas E., Møller-Jensen, Jakob, Olsen, John E., Herrero-Fresno, Ana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8767336/
https://www.ncbi.nlm.nih.gov/pubmed/34928200
http://dx.doi.org/10.1099/mgen.0.000719
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author García, Vanesa
Grønnemose, Rasmus B.
Torres-Puig, Sergi
Kudirkiene, Egle
Piantelli, Mateo
Ahmed, Shahana
Andersen, Thomas E.
Møller-Jensen, Jakob
Olsen, John E.
Herrero-Fresno, Ana
author_facet García, Vanesa
Grønnemose, Rasmus B.
Torres-Puig, Sergi
Kudirkiene, Egle
Piantelli, Mateo
Ahmed, Shahana
Andersen, Thomas E.
Møller-Jensen, Jakob
Olsen, John E.
Herrero-Fresno, Ana
author_sort García, Vanesa
collection PubMed
description Uropathogenic Escherichia coli (UPEC) UTI89 is a well-characterized strain, which has mainly been used to study UPEC virulence during urinary tract infection (UTI). However, little is known on UTI89 key fitness-factors during growth in lab media and during UTI. Here, we used a transposon-insertion-sequencing approach (TraDIS) to reveal the UTI89 essential-genes for in vitro growth and fitness-gene-sets for growth in Luria broth (LB) and EZ-MOPS medium without glucose, as well as for human bacteriuria and mouse cystitis. A total of 293 essential genes for growth were identified and the set of fitness-genes was shown to differ depending on the growth media. A modified, previously validated UTI murine model, with administration of glucose prior to infection was applied. Selected fitness-genes for growth in urine and mouse-bladder colonization were validated using deletion-mutants. Novel fitness-genes, such as tusA, corA and rfaG; involved in sulphur-acquisition, magnesium-uptake, and LPS-biosynthesis, were proved to be important during UTI. Moreover, rfaG was confirmed as relevant in both niches, and therefore it may represent a target for novel UTI-treatment/prevention strategies.
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spelling pubmed-87673362022-01-19 Genome-wide analysis of fitness-factors in uropathogenic Escherichia coli during growth in laboratory media and during urinary tract infections García, Vanesa Grønnemose, Rasmus B. Torres-Puig, Sergi Kudirkiene, Egle Piantelli, Mateo Ahmed, Shahana Andersen, Thomas E. Møller-Jensen, Jakob Olsen, John E. Herrero-Fresno, Ana Microb Genom Research Articles Uropathogenic Escherichia coli (UPEC) UTI89 is a well-characterized strain, which has mainly been used to study UPEC virulence during urinary tract infection (UTI). However, little is known on UTI89 key fitness-factors during growth in lab media and during UTI. Here, we used a transposon-insertion-sequencing approach (TraDIS) to reveal the UTI89 essential-genes for in vitro growth and fitness-gene-sets for growth in Luria broth (LB) and EZ-MOPS medium without glucose, as well as for human bacteriuria and mouse cystitis. A total of 293 essential genes for growth were identified and the set of fitness-genes was shown to differ depending on the growth media. A modified, previously validated UTI murine model, with administration of glucose prior to infection was applied. Selected fitness-genes for growth in urine and mouse-bladder colonization were validated using deletion-mutants. Novel fitness-genes, such as tusA, corA and rfaG; involved in sulphur-acquisition, magnesium-uptake, and LPS-biosynthesis, were proved to be important during UTI. Moreover, rfaG was confirmed as relevant in both niches, and therefore it may represent a target for novel UTI-treatment/prevention strategies. Microbiology Society 2021-12-20 /pmc/articles/PMC8767336/ /pubmed/34928200 http://dx.doi.org/10.1099/mgen.0.000719 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License.
spellingShingle Research Articles
García, Vanesa
Grønnemose, Rasmus B.
Torres-Puig, Sergi
Kudirkiene, Egle
Piantelli, Mateo
Ahmed, Shahana
Andersen, Thomas E.
Møller-Jensen, Jakob
Olsen, John E.
Herrero-Fresno, Ana
Genome-wide analysis of fitness-factors in uropathogenic Escherichia coli during growth in laboratory media and during urinary tract infections
title Genome-wide analysis of fitness-factors in uropathogenic Escherichia coli during growth in laboratory media and during urinary tract infections
title_full Genome-wide analysis of fitness-factors in uropathogenic Escherichia coli during growth in laboratory media and during urinary tract infections
title_fullStr Genome-wide analysis of fitness-factors in uropathogenic Escherichia coli during growth in laboratory media and during urinary tract infections
title_full_unstemmed Genome-wide analysis of fitness-factors in uropathogenic Escherichia coli during growth in laboratory media and during urinary tract infections
title_short Genome-wide analysis of fitness-factors in uropathogenic Escherichia coli during growth in laboratory media and during urinary tract infections
title_sort genome-wide analysis of fitness-factors in uropathogenic escherichia coli during growth in laboratory media and during urinary tract infections
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8767336/
https://www.ncbi.nlm.nih.gov/pubmed/34928200
http://dx.doi.org/10.1099/mgen.0.000719
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