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Genomic insights into the circulation of pandemic fluoroquinolone-resistant extra-intestinal pathogenic Escherichia coli ST1193 in Vietnam
Extra-intestinal pathogenic Escherichia coli (ExPEC) ST1193, a globally emergent fluoroquinolone-resistant clone, has become an important cause of bloodstream infections (BSIs) associated with significant morbidity and mortality. Previous studies have reported the emergence of fluoroquinolone-resist...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Microbiology Society
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8767341/ https://www.ncbi.nlm.nih.gov/pubmed/34904942 http://dx.doi.org/10.1099/mgen.0.000733 |
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author | Nguyen, Quynh Nguyen, To Thi Nguyen Pham, Phuong Chau, Vinh Nguyen, Lan Phu Huong Nguyen, Toan Duc Ha, Tuyen Thanh Le, Nhi Thi Quynh Vu, Duong Thuy Baker, Stephen Thwaites, Guy E. Rabaa, Maia A. Pham, Duy Thanh |
author_facet | Nguyen, Quynh Nguyen, To Thi Nguyen Pham, Phuong Chau, Vinh Nguyen, Lan Phu Huong Nguyen, Toan Duc Ha, Tuyen Thanh Le, Nhi Thi Quynh Vu, Duong Thuy Baker, Stephen Thwaites, Guy E. Rabaa, Maia A. Pham, Duy Thanh |
author_sort | Nguyen, Quynh |
collection | PubMed |
description | Extra-intestinal pathogenic Escherichia coli (ExPEC) ST1193, a globally emergent fluoroquinolone-resistant clone, has become an important cause of bloodstream infections (BSIs) associated with significant morbidity and mortality. Previous studies have reported the emergence of fluoroquinolone-resistant ExPEC ST1193 in Vietnam; however, limited data exist regarding the genetic structure, antimicrobial resistance (AMR) determinants and transmission dynamics of this pandemic clone. Here, we performed genomic and phylogenetic analyses of 46 ST1193 isolates obtained from BSIs and healthy individuals in Ho Chi Minh City, Vietnam, to investigate the pathogen population structure, molecular mechanisms of AMR and potential transmission patterns. We further examined the phylogenetic structure of ST1193 isolates in a global context. We found that the endemic E. coli ST1193 population was heterogeneous and highly dynamic, largely driven by multiple strain importations. Several well-supported phylogenetic clusters (C1–C6) were identified and associated with distinct bla (CTX-M) variants, including bla (CTXM-27) (C1–C3, C5), bla (CTXM-55) (C4) and bla (CTXM-15) (C6). Most ST1193 isolates were multidrug-resistant and carried an extensive array of AMR genes. ST1193 isolates also exhibited the ability to acquire further resistance while circulating in Vietnam. There were phylogenetic links between ST1193 isolates from BSIs and healthy individuals, suggesting these organisms may both establish long-term colonization in the human intestinal tract and induce infections. Our study uncovers factors shaping the population structure and transmission dynamics of multidrug-resistant ST1193 in Vietnam, and highlights the urgent need for local One Health genomic surveillance to capture new emerging ExPEC clones and to better understand the origins and transmission patterns of these pathogens. |
format | Online Article Text |
id | pubmed-8767341 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Microbiology Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-87673412022-01-19 Genomic insights into the circulation of pandemic fluoroquinolone-resistant extra-intestinal pathogenic Escherichia coli ST1193 in Vietnam Nguyen, Quynh Nguyen, To Thi Nguyen Pham, Phuong Chau, Vinh Nguyen, Lan Phu Huong Nguyen, Toan Duc Ha, Tuyen Thanh Le, Nhi Thi Quynh Vu, Duong Thuy Baker, Stephen Thwaites, Guy E. Rabaa, Maia A. Pham, Duy Thanh Microb Genom Short Communications Extra-intestinal pathogenic Escherichia coli (ExPEC) ST1193, a globally emergent fluoroquinolone-resistant clone, has become an important cause of bloodstream infections (BSIs) associated with significant morbidity and mortality. Previous studies have reported the emergence of fluoroquinolone-resistant ExPEC ST1193 in Vietnam; however, limited data exist regarding the genetic structure, antimicrobial resistance (AMR) determinants and transmission dynamics of this pandemic clone. Here, we performed genomic and phylogenetic analyses of 46 ST1193 isolates obtained from BSIs and healthy individuals in Ho Chi Minh City, Vietnam, to investigate the pathogen population structure, molecular mechanisms of AMR and potential transmission patterns. We further examined the phylogenetic structure of ST1193 isolates in a global context. We found that the endemic E. coli ST1193 population was heterogeneous and highly dynamic, largely driven by multiple strain importations. Several well-supported phylogenetic clusters (C1–C6) were identified and associated with distinct bla (CTX-M) variants, including bla (CTXM-27) (C1–C3, C5), bla (CTXM-55) (C4) and bla (CTXM-15) (C6). Most ST1193 isolates were multidrug-resistant and carried an extensive array of AMR genes. ST1193 isolates also exhibited the ability to acquire further resistance while circulating in Vietnam. There were phylogenetic links between ST1193 isolates from BSIs and healthy individuals, suggesting these organisms may both establish long-term colonization in the human intestinal tract and induce infections. Our study uncovers factors shaping the population structure and transmission dynamics of multidrug-resistant ST1193 in Vietnam, and highlights the urgent need for local One Health genomic surveillance to capture new emerging ExPEC clones and to better understand the origins and transmission patterns of these pathogens. Microbiology Society 2021-12-14 /pmc/articles/PMC8767341/ /pubmed/34904942 http://dx.doi.org/10.1099/mgen.0.000733 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License. |
spellingShingle | Short Communications Nguyen, Quynh Nguyen, To Thi Nguyen Pham, Phuong Chau, Vinh Nguyen, Lan Phu Huong Nguyen, Toan Duc Ha, Tuyen Thanh Le, Nhi Thi Quynh Vu, Duong Thuy Baker, Stephen Thwaites, Guy E. Rabaa, Maia A. Pham, Duy Thanh Genomic insights into the circulation of pandemic fluoroquinolone-resistant extra-intestinal pathogenic Escherichia coli ST1193 in Vietnam |
title | Genomic insights into the circulation of pandemic fluoroquinolone-resistant extra-intestinal pathogenic Escherichia coli ST1193 in Vietnam |
title_full | Genomic insights into the circulation of pandemic fluoroquinolone-resistant extra-intestinal pathogenic Escherichia coli ST1193 in Vietnam |
title_fullStr | Genomic insights into the circulation of pandemic fluoroquinolone-resistant extra-intestinal pathogenic Escherichia coli ST1193 in Vietnam |
title_full_unstemmed | Genomic insights into the circulation of pandemic fluoroquinolone-resistant extra-intestinal pathogenic Escherichia coli ST1193 in Vietnam |
title_short | Genomic insights into the circulation of pandemic fluoroquinolone-resistant extra-intestinal pathogenic Escherichia coli ST1193 in Vietnam |
title_sort | genomic insights into the circulation of pandemic fluoroquinolone-resistant extra-intestinal pathogenic escherichia coli st1193 in vietnam |
topic | Short Communications |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8767341/ https://www.ncbi.nlm.nih.gov/pubmed/34904942 http://dx.doi.org/10.1099/mgen.0.000733 |
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