Cargando…
Acidovorax pan-genome reveals specific functional traits for plant beneficial and pathogenic plant-associations
Beta-proteobacteria belonging to the genus Acidovorax have been described from various environments. Many strains can interact with a range of hosts, including humans and plants, forming neutral, beneficial or detrimental associations. In the frame of this study, we investigated the genomic properti...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Microbiology Society
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8767351/ https://www.ncbi.nlm.nih.gov/pubmed/34889729 http://dx.doi.org/10.1099/mgen.0.000666 |
_version_ | 1784634719131926528 |
---|---|
author | Siani, Roberto Stabl, Georg Gutjahr, Caroline Schloter, Michael Radl, Viviane |
author_facet | Siani, Roberto Stabl, Georg Gutjahr, Caroline Schloter, Michael Radl, Viviane |
author_sort | Siani, Roberto |
collection | PubMed |
description | Beta-proteobacteria belonging to the genus Acidovorax have been described from various environments. Many strains can interact with a range of hosts, including humans and plants, forming neutral, beneficial or detrimental associations. In the frame of this study, we investigated the genomic properties of 52 bacterial strains of the genus Acidovorax , isolated from healthy roots of Lotus japonicus, with the intent of identifying traits important for effective plant-growth promotion. Based on single-strain inoculation bioassays with L. japonicus, performed in a gnotobiotic system, we distinguished seven robust plant-growth promoting strains from strains with no significant effects on plant-growth. We showed that the genomes of the two groups differed prominently in protein families linked to sensing and transport of organic acids, production of phytohormones, as well as resistance and production of compounds with antimicrobial properties. In a second step, we compared the genomes of the tested isolates with those of plant pathogens and free-living strains of the genus Acidovorax sourced from public repositories. Our pan-genomics comparison revealed features correlated with commensal and pathogenic lifestyle. We showed that commensals and pathogens differ mostly in their ability to use plant-derived lipids and in the type of secretion-systems being present. Most free-living Acidovorax strains did not harbour any secretion-systems. Overall, our data indicate that Acidovorax strains undergo extensive adaptations to their particular lifestyle by horizontal uptake of novel genetic information and loss of unnecessary genes. |
format | Online Article Text |
id | pubmed-8767351 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Microbiology Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-87673512022-01-19 Acidovorax pan-genome reveals specific functional traits for plant beneficial and pathogenic plant-associations Siani, Roberto Stabl, Georg Gutjahr, Caroline Schloter, Michael Radl, Viviane Microb Genom Research Articles Beta-proteobacteria belonging to the genus Acidovorax have been described from various environments. Many strains can interact with a range of hosts, including humans and plants, forming neutral, beneficial or detrimental associations. In the frame of this study, we investigated the genomic properties of 52 bacterial strains of the genus Acidovorax , isolated from healthy roots of Lotus japonicus, with the intent of identifying traits important for effective plant-growth promotion. Based on single-strain inoculation bioassays with L. japonicus, performed in a gnotobiotic system, we distinguished seven robust plant-growth promoting strains from strains with no significant effects on plant-growth. We showed that the genomes of the two groups differed prominently in protein families linked to sensing and transport of organic acids, production of phytohormones, as well as resistance and production of compounds with antimicrobial properties. In a second step, we compared the genomes of the tested isolates with those of plant pathogens and free-living strains of the genus Acidovorax sourced from public repositories. Our pan-genomics comparison revealed features correlated with commensal and pathogenic lifestyle. We showed that commensals and pathogens differ mostly in their ability to use plant-derived lipids and in the type of secretion-systems being present. Most free-living Acidovorax strains did not harbour any secretion-systems. Overall, our data indicate that Acidovorax strains undergo extensive adaptations to their particular lifestyle by horizontal uptake of novel genetic information and loss of unnecessary genes. Microbiology Society 2021-12-10 /pmc/articles/PMC8767351/ /pubmed/34889729 http://dx.doi.org/10.1099/mgen.0.000666 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License. |
spellingShingle | Research Articles Siani, Roberto Stabl, Georg Gutjahr, Caroline Schloter, Michael Radl, Viviane Acidovorax pan-genome reveals specific functional traits for plant beneficial and pathogenic plant-associations |
title |
Acidovorax pan-genome reveals specific functional traits for plant beneficial and pathogenic plant-associations |
title_full |
Acidovorax pan-genome reveals specific functional traits for plant beneficial and pathogenic plant-associations |
title_fullStr |
Acidovorax pan-genome reveals specific functional traits for plant beneficial and pathogenic plant-associations |
title_full_unstemmed |
Acidovorax pan-genome reveals specific functional traits for plant beneficial and pathogenic plant-associations |
title_short |
Acidovorax pan-genome reveals specific functional traits for plant beneficial and pathogenic plant-associations |
title_sort | acidovorax pan-genome reveals specific functional traits for plant beneficial and pathogenic plant-associations |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8767351/ https://www.ncbi.nlm.nih.gov/pubmed/34889729 http://dx.doi.org/10.1099/mgen.0.000666 |
work_keys_str_mv | AT sianiroberto acidovoraxpangenomerevealsspecificfunctionaltraitsforplantbeneficialandpathogenicplantassociations AT stablgeorg acidovoraxpangenomerevealsspecificfunctionaltraitsforplantbeneficialandpathogenicplantassociations AT gutjahrcaroline acidovoraxpangenomerevealsspecificfunctionaltraitsforplantbeneficialandpathogenicplantassociations AT schlotermichael acidovoraxpangenomerevealsspecificfunctionaltraitsforplantbeneficialandpathogenicplantassociations AT radlviviane acidovoraxpangenomerevealsspecificfunctionaltraitsforplantbeneficialandpathogenicplantassociations |