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Anion binding to a cationic europium(iii) probe enables the first real-time assay of heparan sulfotransferase activity
Sulfotransferases constitute a ubiquitous class of enzymes which are poorly understood due to the lack of a convenient tool for screening their activity. These enzymes use the anion PAPS (adenosine-3′-phosphate-5′-phosphosulfate) as a donor for a broad range of acceptor substrates, including carbohy...
Autores principales: | Wheeler, Simon, Breen, Colum, Li, Yong, Hewitt, Sarah H., Robertson, Erin, Yates, Edwin A., Barsukov, Igor L., Fernig, David G., Butler, Stephen J. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8767414/ https://www.ncbi.nlm.nih.gov/pubmed/34951618 http://dx.doi.org/10.1039/d1ob02071d |
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