Ubiquitous Knockdown of Mettl3 using TRiP.GL01126 Results in Spermatid Mislocalization During Drosophila Spermatogenesis

METTL3, the enzyme that catalyzes the m(6)A RNA modification in Drosophila is highly conserved and essential in various eukaryotic organisms. Mettl3 and its homologs have been linked to biological processes such as gametogenesis. We focused on characterizing the role of METTL3 in Drosophila spermato...

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Detalles Bibliográficos
Autores principales: Rowe, Lauren, Rockwell, Antonio L
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2022
Materias:
New Finding
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8767421/
https://www.ncbi.nlm.nih.gov/pubmed/35071998
http://dx.doi.org/10.17912/micropub.biology.000511
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author Rowe, Lauren
Rockwell, Antonio L
author_facet Rowe, Lauren
Rockwell, Antonio L
author_sort Rowe, Lauren
collection PubMed
description METTL3, the enzyme that catalyzes the m(6)A RNA modification in Drosophila is highly conserved and essential in various eukaryotic organisms. Mettl3 and its homologs have been linked to biological processes such as gametogenesis. We focused on characterizing the role of METTL3 in Drosophila spermatogenesis. We used the Gal4-UAS system to ubiquitously knockdown Mettl3 in both somatic cyst cells and germline cells. Using immunostaining and confocal microscopy, we found spermatid bundles mislocalize in testes that contain the morphologically abnormal swollen apical tip. Our result suggests Mettl3 knockdown using TRiP.GL01126 results in spermatogenesis aberrations.
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spelling pubmed-87674212022-01-20 Ubiquitous Knockdown of Mettl3 using TRiP.GL01126 Results in Spermatid Mislocalization During Drosophila Spermatogenesis Rowe, Lauren Rockwell, Antonio L MicroPubl Biol New Finding METTL3, the enzyme that catalyzes the m(6)A RNA modification in Drosophila is highly conserved and essential in various eukaryotic organisms. Mettl3 and its homologs have been linked to biological processes such as gametogenesis. We focused on characterizing the role of METTL3 in Drosophila spermatogenesis. We used the Gal4-UAS system to ubiquitously knockdown Mettl3 in both somatic cyst cells and germline cells. Using immunostaining and confocal microscopy, we found spermatid bundles mislocalize in testes that contain the morphologically abnormal swollen apical tip. Our result suggests Mettl3 knockdown using TRiP.GL01126 results in spermatogenesis aberrations. Caltech Library 2022-01-18 /pmc/articles/PMC8767421/ /pubmed/35071998 http://dx.doi.org/10.17912/micropub.biology.000511 Text en Copyright: © 2022 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle New Finding
Rowe, Lauren
Rockwell, Antonio L
Ubiquitous Knockdown of Mettl3 using TRiP.GL01126 Results in Spermatid Mislocalization During Drosophila Spermatogenesis
title Ubiquitous Knockdown of Mettl3 using TRiP.GL01126 Results in Spermatid Mislocalization During Drosophila Spermatogenesis
title_full Ubiquitous Knockdown of Mettl3 using TRiP.GL01126 Results in Spermatid Mislocalization During Drosophila Spermatogenesis
title_fullStr Ubiquitous Knockdown of Mettl3 using TRiP.GL01126 Results in Spermatid Mislocalization During Drosophila Spermatogenesis
title_full_unstemmed Ubiquitous Knockdown of Mettl3 using TRiP.GL01126 Results in Spermatid Mislocalization During Drosophila Spermatogenesis
title_short Ubiquitous Knockdown of Mettl3 using TRiP.GL01126 Results in Spermatid Mislocalization During Drosophila Spermatogenesis
title_sort ubiquitous knockdown of mettl3 using trip.gl01126 results in spermatid mislocalization during drosophila spermatogenesis
topic New Finding
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8767421/
https://www.ncbi.nlm.nih.gov/pubmed/35071998
http://dx.doi.org/10.17912/micropub.biology.000511
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