Cargando…

Production of succinic acid through the fermentation of Actinobacillus succinogenes on the hydrolysate of Napier grass

BACKGROUND: Napier grass biomass can be hydrolyzed mainly containing glucose and xylose after alkaline pretreatment and enzymatic hydrolysis. This biomass can be fermented using Actinobacillus succinogenes to produce succinic acid. The yield of succinic acid was 0.58 g/g. Because metabolizing xylose...

Descripción completa

Detalles Bibliográficos
Autores principales: Lee, Jhih-Sing, Lin, Cheng-Jia, Lee, Wen-Chien, Teng, Hsin-Yi, Chuang, Meng-Hsin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8767706/
https://www.ncbi.nlm.nih.gov/pubmed/35418147
http://dx.doi.org/10.1186/s13068-022-02106-0
_version_ 1784634790094307328
author Lee, Jhih-Sing
Lin, Cheng-Jia
Lee, Wen-Chien
Teng, Hsin-Yi
Chuang, Meng-Hsin
author_facet Lee, Jhih-Sing
Lin, Cheng-Jia
Lee, Wen-Chien
Teng, Hsin-Yi
Chuang, Meng-Hsin
author_sort Lee, Jhih-Sing
collection PubMed
description BACKGROUND: Napier grass biomass can be hydrolyzed mainly containing glucose and xylose after alkaline pretreatment and enzymatic hydrolysis. This biomass can be fermented using Actinobacillus succinogenes to produce succinic acid. The yield of succinic acid was 0.58 g/g. Because metabolizing xylose could produce more acetic acid, this yield of succinic acid was lower than that achieved using glucose as the sole carbon source. RESULTS: The addition of glycerol as a fermentation substrate to Napier grass hydrolysate increased the reducing power of the hydrolysate, which not only increased the production of succinic acid but also reduced the formation of undesirable acetic acid in bacterial cells. At a hydrolysate:glycerol ratio of 10:1, the succinic acid yield reached 0.65 g/g. The succinic acid yield increased to 0.88 g/g when a 1:1 ratio of hydrolysate:glycerol was used. For the recovery of succinic acid from the fermentation broth, an outside-in module of an ultrafiltration membrane was used to remove bacterial cells. Air sparging at the feed side with a flow rate of 3 L/min increased the filtration rate. When the air flow rate was increased from 0 to 3 L/min, the average filtration rate increased from 25.0 to 45.7 mL/min, which corresponds to an increase of 82.8%. The clarified fermentation broth was then electrodialized to separate succinate from other contaminated ions. After electrodialysis, the acid products were concentrated through water removal, decolorized through treatment with activated carbon, and precipitated to obtain a purified product. CONCLUSIONS: The yield of succinic acid was increased by adding glycerol to the hydrolysate of Napier grass. The downstream processing consisting of ultrafiltration membrane separation and single-stage electrodialysis was effective for product separation and purification. An overall recovery yield of 74.7% ± 4.5% and a purity of 99.4% ± 0.1% were achieved for succinic acid. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13068-022-02106-0.
format Online
Article
Text
id pubmed-8767706
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-87677062022-01-19 Production of succinic acid through the fermentation of Actinobacillus succinogenes on the hydrolysate of Napier grass Lee, Jhih-Sing Lin, Cheng-Jia Lee, Wen-Chien Teng, Hsin-Yi Chuang, Meng-Hsin Biotechnol Biofuels Bioprod Research BACKGROUND: Napier grass biomass can be hydrolyzed mainly containing glucose and xylose after alkaline pretreatment and enzymatic hydrolysis. This biomass can be fermented using Actinobacillus succinogenes to produce succinic acid. The yield of succinic acid was 0.58 g/g. Because metabolizing xylose could produce more acetic acid, this yield of succinic acid was lower than that achieved using glucose as the sole carbon source. RESULTS: The addition of glycerol as a fermentation substrate to Napier grass hydrolysate increased the reducing power of the hydrolysate, which not only increased the production of succinic acid but also reduced the formation of undesirable acetic acid in bacterial cells. At a hydrolysate:glycerol ratio of 10:1, the succinic acid yield reached 0.65 g/g. The succinic acid yield increased to 0.88 g/g when a 1:1 ratio of hydrolysate:glycerol was used. For the recovery of succinic acid from the fermentation broth, an outside-in module of an ultrafiltration membrane was used to remove bacterial cells. Air sparging at the feed side with a flow rate of 3 L/min increased the filtration rate. When the air flow rate was increased from 0 to 3 L/min, the average filtration rate increased from 25.0 to 45.7 mL/min, which corresponds to an increase of 82.8%. The clarified fermentation broth was then electrodialized to separate succinate from other contaminated ions. After electrodialysis, the acid products were concentrated through water removal, decolorized through treatment with activated carbon, and precipitated to obtain a purified product. CONCLUSIONS: The yield of succinic acid was increased by adding glycerol to the hydrolysate of Napier grass. The downstream processing consisting of ultrafiltration membrane separation and single-stage electrodialysis was effective for product separation and purification. An overall recovery yield of 74.7% ± 4.5% and a purity of 99.4% ± 0.1% were achieved for succinic acid. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13068-022-02106-0. BioMed Central 2022-01-18 /pmc/articles/PMC8767706/ /pubmed/35418147 http://dx.doi.org/10.1186/s13068-022-02106-0 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Lee, Jhih-Sing
Lin, Cheng-Jia
Lee, Wen-Chien
Teng, Hsin-Yi
Chuang, Meng-Hsin
Production of succinic acid through the fermentation of Actinobacillus succinogenes on the hydrolysate of Napier grass
title Production of succinic acid through the fermentation of Actinobacillus succinogenes on the hydrolysate of Napier grass
title_full Production of succinic acid through the fermentation of Actinobacillus succinogenes on the hydrolysate of Napier grass
title_fullStr Production of succinic acid through the fermentation of Actinobacillus succinogenes on the hydrolysate of Napier grass
title_full_unstemmed Production of succinic acid through the fermentation of Actinobacillus succinogenes on the hydrolysate of Napier grass
title_short Production of succinic acid through the fermentation of Actinobacillus succinogenes on the hydrolysate of Napier grass
title_sort production of succinic acid through the fermentation of actinobacillus succinogenes on the hydrolysate of napier grass
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8767706/
https://www.ncbi.nlm.nih.gov/pubmed/35418147
http://dx.doi.org/10.1186/s13068-022-02106-0
work_keys_str_mv AT leejhihsing productionofsuccinicacidthroughthefermentationofactinobacillussuccinogenesonthehydrolysateofnapiergrass
AT linchengjia productionofsuccinicacidthroughthefermentationofactinobacillussuccinogenesonthehydrolysateofnapiergrass
AT leewenchien productionofsuccinicacidthroughthefermentationofactinobacillussuccinogenesonthehydrolysateofnapiergrass
AT tenghsinyi productionofsuccinicacidthroughthefermentationofactinobacillussuccinogenesonthehydrolysateofnapiergrass
AT chuangmenghsin productionofsuccinicacidthroughthefermentationofactinobacillussuccinogenesonthehydrolysateofnapiergrass