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Viral Culture Confirmed SARS-CoV-2 Subgenomic RNA Value as a Good Surrogate Marker of Infectivity
Determining SARS-CoV-2 viral infectivity is crucial for patient clinical assessment and isolation decisions. We assessed subgenomic RNA (sgRNA) as a surrogate marker of SARS-CoV-2 infectivity in SARS-CoV-2-positive reverse transcription PCR (RT-PCR) respiratory samples (n = 105) in comparison with v...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8769718/ https://www.ncbi.nlm.nih.gov/pubmed/34669457 http://dx.doi.org/10.1128/JCM.01609-21 |
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author | Santos Bravo, Marta Berengua, Carla Marín, Pilar Esteban, Montserrat Rodriguez, Cristina del Cuerpo, Margarita Miró, Elisenda Cuesta, Genoveva Mosquera, Mar Sánchez-Palomino, Sonsoles Vila, Jordi Rabella, Núria Marcos, María Ángeles |
author_facet | Santos Bravo, Marta Berengua, Carla Marín, Pilar Esteban, Montserrat Rodriguez, Cristina del Cuerpo, Margarita Miró, Elisenda Cuesta, Genoveva Mosquera, Mar Sánchez-Palomino, Sonsoles Vila, Jordi Rabella, Núria Marcos, María Ángeles |
author_sort | Santos Bravo, Marta |
collection | PubMed |
description | Determining SARS-CoV-2 viral infectivity is crucial for patient clinical assessment and isolation decisions. We assessed subgenomic RNA (sgRNA) as a surrogate marker of SARS-CoV-2 infectivity in SARS-CoV-2-positive reverse transcription PCR (RT-PCR) respiratory samples (n = 105) in comparison with viral culture as the reference standard for virus replication. sgRNA and viral isolation results were concordant in 99/105 cases (94%), indicating highly significant agreement between the two techniques (Cohen’s kappa coefficient 0.88, 95% confidence interval [CI] 0.78 to 0.97, P < 0.001). sgRNA RT-PCR showed a sensitivity of 97% and a positive predictive value of 94% to detect replication-competent virus, further supporting sgRNA as a surrogate marker of SARS-CoV-2 infectivity. sgRNA RT-PCR is an accurate, rapid, and affordable technique that can overcome culture and cycle threshold (C(T)) value limitations and be routinely implemented in hospital laboratories to detect viral infectivity, which is essential for optimizing patient monitoring, the efficacy of treatments/vaccines, and work reincorporation policies, as well as for safely shortening isolation precautions. |
format | Online Article Text |
id | pubmed-8769718 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-87697182022-02-09 Viral Culture Confirmed SARS-CoV-2 Subgenomic RNA Value as a Good Surrogate Marker of Infectivity Santos Bravo, Marta Berengua, Carla Marín, Pilar Esteban, Montserrat Rodriguez, Cristina del Cuerpo, Margarita Miró, Elisenda Cuesta, Genoveva Mosquera, Mar Sánchez-Palomino, Sonsoles Vila, Jordi Rabella, Núria Marcos, María Ángeles J Clin Microbiol Virology Determining SARS-CoV-2 viral infectivity is crucial for patient clinical assessment and isolation decisions. We assessed subgenomic RNA (sgRNA) as a surrogate marker of SARS-CoV-2 infectivity in SARS-CoV-2-positive reverse transcription PCR (RT-PCR) respiratory samples (n = 105) in comparison with viral culture as the reference standard for virus replication. sgRNA and viral isolation results were concordant in 99/105 cases (94%), indicating highly significant agreement between the two techniques (Cohen’s kappa coefficient 0.88, 95% confidence interval [CI] 0.78 to 0.97, P < 0.001). sgRNA RT-PCR showed a sensitivity of 97% and a positive predictive value of 94% to detect replication-competent virus, further supporting sgRNA as a surrogate marker of SARS-CoV-2 infectivity. sgRNA RT-PCR is an accurate, rapid, and affordable technique that can overcome culture and cycle threshold (C(T)) value limitations and be routinely implemented in hospital laboratories to detect viral infectivity, which is essential for optimizing patient monitoring, the efficacy of treatments/vaccines, and work reincorporation policies, as well as for safely shortening isolation precautions. American Society for Microbiology 2022-01-19 /pmc/articles/PMC8769718/ /pubmed/34669457 http://dx.doi.org/10.1128/JCM.01609-21 Text en Copyright © 2022 American Society for Microbiology. https://doi.org/10.1128/ASMCopyrightv2All Rights Reserved (https://doi.org/10.1128/ASMCopyrightv2) . https://doi.org/10.1128/ASMCopyrightv2This article is made available via the PMC Open Access Subset for unrestricted noncommercial re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Virology Santos Bravo, Marta Berengua, Carla Marín, Pilar Esteban, Montserrat Rodriguez, Cristina del Cuerpo, Margarita Miró, Elisenda Cuesta, Genoveva Mosquera, Mar Sánchez-Palomino, Sonsoles Vila, Jordi Rabella, Núria Marcos, María Ángeles Viral Culture Confirmed SARS-CoV-2 Subgenomic RNA Value as a Good Surrogate Marker of Infectivity |
title | Viral Culture Confirmed SARS-CoV-2 Subgenomic RNA Value as a Good Surrogate Marker of Infectivity |
title_full | Viral Culture Confirmed SARS-CoV-2 Subgenomic RNA Value as a Good Surrogate Marker of Infectivity |
title_fullStr | Viral Culture Confirmed SARS-CoV-2 Subgenomic RNA Value as a Good Surrogate Marker of Infectivity |
title_full_unstemmed | Viral Culture Confirmed SARS-CoV-2 Subgenomic RNA Value as a Good Surrogate Marker of Infectivity |
title_short | Viral Culture Confirmed SARS-CoV-2 Subgenomic RNA Value as a Good Surrogate Marker of Infectivity |
title_sort | viral culture confirmed sars-cov-2 subgenomic rna value as a good surrogate marker of infectivity |
topic | Virology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8769718/ https://www.ncbi.nlm.nih.gov/pubmed/34669457 http://dx.doi.org/10.1128/JCM.01609-21 |
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