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High-Density Single Nucleotide Polymorphisms Genetic Map Construction and Quantitative Trait Locus Mapping of Color-Related Traits of Purple Sweet Potato [Ipomoea batatas (L.) Lam.]

Flesh color (FC), skin color (SC), and anthocyanin content (AC) are three important traits being used for commodity evaluation in purple-fleshed sweet potato. However, to date, only a few reports are available on the inheritance of these traits. In this study, we used a biparental mapping population...

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Autores principales: Yan, Hui, Ma, Meng, Ahmad, Muhammad Qadir, Arisha, Mohamed Hamed, Tang, Wei, Li, Chen, Zhang, Yungang, Kou, Meng, Wang, Xin, Gao, Runfei, Song, Weihan, Li, Zongyun, Li, Qiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8770336/
https://www.ncbi.nlm.nih.gov/pubmed/35069654
http://dx.doi.org/10.3389/fpls.2021.797041
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author Yan, Hui
Ma, Meng
Ahmad, Muhammad Qadir
Arisha, Mohamed Hamed
Tang, Wei
Li, Chen
Zhang, Yungang
Kou, Meng
Wang, Xin
Gao, Runfei
Song, Weihan
Li, Zongyun
Li, Qiang
author_facet Yan, Hui
Ma, Meng
Ahmad, Muhammad Qadir
Arisha, Mohamed Hamed
Tang, Wei
Li, Chen
Zhang, Yungang
Kou, Meng
Wang, Xin
Gao, Runfei
Song, Weihan
Li, Zongyun
Li, Qiang
author_sort Yan, Hui
collection PubMed
description Flesh color (FC), skin color (SC), and anthocyanin content (AC) are three important traits being used for commodity evaluation in purple-fleshed sweet potato. However, to date, only a few reports are available on the inheritance of these traits. In this study, we used a biparental mapping population of 274 F1 progeny generated from a cross between a dark purple-fleshed (Xuzishu8) and white-fleshed (Meiguohong) sweet potato variety for genetic analyses. Correlation analysis showed a significant positive correlation among AC, SC, and FC. Medium-to-high heritability was observed for these traits. We detected single nucleotide polymorphisms (SNPs) by specific length amplified fragment sequencing (SLAF-seq) with the average sequencing depth of 51.72 and 25.76 for parents and progeny, respectively. Then we constructed an integrated genetic map consisting of 15 linkage groups (LGS) of sweet potato spanning on 2,233.66 cm with an average map distance of 0.71 cm between adjacent markers. Based on the linkage map, ten major quantitative trait loci (QTLs) associated to FC, SC, and AC were identified on LG12 between 0 and 64.97 cm distance, such as one QTL for SC and FC, respectively, which explained 36.3 and 45.9% of phenotypic variation; eight QTLs for AC, which explained 10.5–28.5% of the variation. These major QTLs were highly consistent and co-localized on LG12. Positive correlation, high heritability, and co-localization of QTLs on the same LG group confirm the significance of this study to establish a marker-assisted breeding program for sweet potato improvement.
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spelling pubmed-87703362022-01-21 High-Density Single Nucleotide Polymorphisms Genetic Map Construction and Quantitative Trait Locus Mapping of Color-Related Traits of Purple Sweet Potato [Ipomoea batatas (L.) Lam.] Yan, Hui Ma, Meng Ahmad, Muhammad Qadir Arisha, Mohamed Hamed Tang, Wei Li, Chen Zhang, Yungang Kou, Meng Wang, Xin Gao, Runfei Song, Weihan Li, Zongyun Li, Qiang Front Plant Sci Plant Science Flesh color (FC), skin color (SC), and anthocyanin content (AC) are three important traits being used for commodity evaluation in purple-fleshed sweet potato. However, to date, only a few reports are available on the inheritance of these traits. In this study, we used a biparental mapping population of 274 F1 progeny generated from a cross between a dark purple-fleshed (Xuzishu8) and white-fleshed (Meiguohong) sweet potato variety for genetic analyses. Correlation analysis showed a significant positive correlation among AC, SC, and FC. Medium-to-high heritability was observed for these traits. We detected single nucleotide polymorphisms (SNPs) by specific length amplified fragment sequencing (SLAF-seq) with the average sequencing depth of 51.72 and 25.76 for parents and progeny, respectively. Then we constructed an integrated genetic map consisting of 15 linkage groups (LGS) of sweet potato spanning on 2,233.66 cm with an average map distance of 0.71 cm between adjacent markers. Based on the linkage map, ten major quantitative trait loci (QTLs) associated to FC, SC, and AC were identified on LG12 between 0 and 64.97 cm distance, such as one QTL for SC and FC, respectively, which explained 36.3 and 45.9% of phenotypic variation; eight QTLs for AC, which explained 10.5–28.5% of the variation. These major QTLs were highly consistent and co-localized on LG12. Positive correlation, high heritability, and co-localization of QTLs on the same LG group confirm the significance of this study to establish a marker-assisted breeding program for sweet potato improvement. Frontiers Media S.A. 2022-01-06 /pmc/articles/PMC8770336/ /pubmed/35069654 http://dx.doi.org/10.3389/fpls.2021.797041 Text en Copyright © 2022 Yan, Ma, Ahmad, Arisha, Tang, Li, Zhang, Kou, Wang, Gao, Song, Li and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Yan, Hui
Ma, Meng
Ahmad, Muhammad Qadir
Arisha, Mohamed Hamed
Tang, Wei
Li, Chen
Zhang, Yungang
Kou, Meng
Wang, Xin
Gao, Runfei
Song, Weihan
Li, Zongyun
Li, Qiang
High-Density Single Nucleotide Polymorphisms Genetic Map Construction and Quantitative Trait Locus Mapping of Color-Related Traits of Purple Sweet Potato [Ipomoea batatas (L.) Lam.]
title High-Density Single Nucleotide Polymorphisms Genetic Map Construction and Quantitative Trait Locus Mapping of Color-Related Traits of Purple Sweet Potato [Ipomoea batatas (L.) Lam.]
title_full High-Density Single Nucleotide Polymorphisms Genetic Map Construction and Quantitative Trait Locus Mapping of Color-Related Traits of Purple Sweet Potato [Ipomoea batatas (L.) Lam.]
title_fullStr High-Density Single Nucleotide Polymorphisms Genetic Map Construction and Quantitative Trait Locus Mapping of Color-Related Traits of Purple Sweet Potato [Ipomoea batatas (L.) Lam.]
title_full_unstemmed High-Density Single Nucleotide Polymorphisms Genetic Map Construction and Quantitative Trait Locus Mapping of Color-Related Traits of Purple Sweet Potato [Ipomoea batatas (L.) Lam.]
title_short High-Density Single Nucleotide Polymorphisms Genetic Map Construction and Quantitative Trait Locus Mapping of Color-Related Traits of Purple Sweet Potato [Ipomoea batatas (L.) Lam.]
title_sort high-density single nucleotide polymorphisms genetic map construction and quantitative trait locus mapping of color-related traits of purple sweet potato [ipomoea batatas (l.) lam.]
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8770336/
https://www.ncbi.nlm.nih.gov/pubmed/35069654
http://dx.doi.org/10.3389/fpls.2021.797041
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