In vitro cytotoxicity analysis of Zizyphus spina-christi stem bark extract on human cancer cell lines

Zizyphus spina-christi (Rhamnaceae family) is an edible plant used in folk medicine. Therefore, it is of interest to report the cytotoxic effects of Z. spina-christi bark crude extract on human cell lines. Crude ethanol extract of Z. spina-christi bark was fractionated with increasing polarity (diet...

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Autores principales: Rajendrasozhan, Saravanan, Ads, Essam N, Abouzied, Amr S, Humaidi, Jamal R
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Biomedical Informatics 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8770409/
https://www.ncbi.nlm.nih.gov/pubmed/35095232
http://dx.doi.org/10.6026/97320630017583
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author Rajendrasozhan, Saravanan
Ads, Essam N
Abouzied, Amr S
Humaidi, Jamal R
author_facet Rajendrasozhan, Saravanan
Ads, Essam N
Abouzied, Amr S
Humaidi, Jamal R
author_sort Rajendrasozhan, Saravanan
collection PubMed
description Zizyphus spina-christi (Rhamnaceae family) is an edible plant used in folk medicine. Therefore, it is of interest to report the cytotoxic effects of Z. spina-christi bark crude extract on human cell lines. Crude ethanol extract of Z. spina-christi bark was fractionated with increasing polarity (diethyl ether, chloroform, ethyl acetate and butanol fractions). The fractions were examined for their cytotoxicity against human colon cancer (HCT-116 and CACO-2), cervical cancer (HeLa and HEp-2), lung carcinoma (A-549), hepatocellular carcinoma (HepG-2), breast cancer (MCF-7) and prostate cancer (PC-3) cell lines using viability assay. Diethyl ether fraction of Z. spina-christi showed the highest cytotoxic effects among the four extracts of Z. spina-christi. The IC50 of diethyl ether fraction was 7.14, 11.2, 11.6, 15.4, 39.8, 42.2, 84.2 and 153.8 µg/ml on HepG-2, A-549, CACO-2, HCT-116, MCF-7, PC-3, HeLa, and HEp-2 cell lines, respectively. Data shows that the diethyl ether fraction of Z. spina-christi showed effective cytotoxic effects in colon, lung and hepatocellular cancer cell lines.
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spelling pubmed-87704092022-01-27 In vitro cytotoxicity analysis of Zizyphus spina-christi stem bark extract on human cancer cell lines Rajendrasozhan, Saravanan Ads, Essam N Abouzied, Amr S Humaidi, Jamal R Bioinformation Research Article Zizyphus spina-christi (Rhamnaceae family) is an edible plant used in folk medicine. Therefore, it is of interest to report the cytotoxic effects of Z. spina-christi bark crude extract on human cell lines. Crude ethanol extract of Z. spina-christi bark was fractionated with increasing polarity (diethyl ether, chloroform, ethyl acetate and butanol fractions). The fractions were examined for their cytotoxicity against human colon cancer (HCT-116 and CACO-2), cervical cancer (HeLa and HEp-2), lung carcinoma (A-549), hepatocellular carcinoma (HepG-2), breast cancer (MCF-7) and prostate cancer (PC-3) cell lines using viability assay. Diethyl ether fraction of Z. spina-christi showed the highest cytotoxic effects among the four extracts of Z. spina-christi. The IC50 of diethyl ether fraction was 7.14, 11.2, 11.6, 15.4, 39.8, 42.2, 84.2 and 153.8 µg/ml on HepG-2, A-549, CACO-2, HCT-116, MCF-7, PC-3, HeLa, and HEp-2 cell lines, respectively. Data shows that the diethyl ether fraction of Z. spina-christi showed effective cytotoxic effects in colon, lung and hepatocellular cancer cell lines. Biomedical Informatics 2021-05-31 /pmc/articles/PMC8770409/ /pubmed/35095232 http://dx.doi.org/10.6026/97320630017583 Text en © 2021 Biomedical Informatics https://creativecommons.org/licenses/by/3.0/This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
spellingShingle Research Article
Rajendrasozhan, Saravanan
Ads, Essam N
Abouzied, Amr S
Humaidi, Jamal R
In vitro cytotoxicity analysis of Zizyphus spina-christi stem bark extract on human cancer cell lines
title In vitro cytotoxicity analysis of Zizyphus spina-christi stem bark extract on human cancer cell lines
title_full In vitro cytotoxicity analysis of Zizyphus spina-christi stem bark extract on human cancer cell lines
title_fullStr In vitro cytotoxicity analysis of Zizyphus spina-christi stem bark extract on human cancer cell lines
title_full_unstemmed In vitro cytotoxicity analysis of Zizyphus spina-christi stem bark extract on human cancer cell lines
title_short In vitro cytotoxicity analysis of Zizyphus spina-christi stem bark extract on human cancer cell lines
title_sort in vitro cytotoxicity analysis of zizyphus spina-christi stem bark extract on human cancer cell lines
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8770409/
https://www.ncbi.nlm.nih.gov/pubmed/35095232
http://dx.doi.org/10.6026/97320630017583
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