Cargando…
Evaluation of Epigenetic Age Based on DNA Methylation Analysis of Several CpG Sites in Ukrainian Population
Epigenetic clocks are the models, which use CpG methylation levels for the age prediction of an organism. Although there were several epigenetic clocks developed there is a demand for development and evaluation of the relatively accurate and sensitive epigenetic clocks that can be used for routine r...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8770732/ https://www.ncbi.nlm.nih.gov/pubmed/35069680 http://dx.doi.org/10.3389/fgene.2021.772298 |
_version_ | 1784635430424018944 |
---|---|
author | Kuzub, N. Smialkovska, V. Momot, V. Moseiko, V. Lushchak, O. Koliada, A. |
author_facet | Kuzub, N. Smialkovska, V. Momot, V. Moseiko, V. Lushchak, O. Koliada, A. |
author_sort | Kuzub, N. |
collection | PubMed |
description | Epigenetic clocks are the models, which use CpG methylation levels for the age prediction of an organism. Although there were several epigenetic clocks developed there is a demand for development and evaluation of the relatively accurate and sensitive epigenetic clocks that can be used for routine research purposes. In this study, we evaluated two epigenetic clock models based on the 4 CpG sites and 2 CpG sites in the human genome using the pyrosequencing method for their methylation level estimation. The study sample included 153 people from the Ukrainian population with the age from 0 to 101. Both models showed a high correlation with the chronological age in our study sample (R(2) = 0.85 for the 2 CpG model and R(2) = 0.92 for the 4 CpG model). We also estimated the accuracy metrics of the age prediction in our study sample. For the age group from 18 to 80 MAD was 5.1 years for the 2 CpG model and 4.1 years for the 4 CpG model. In this regard, we can conclude, that the models evaluated in the study have good age predictive accuracy, and can be used for the epigenetic age evaluation due to the relative simplicity and time-effectiveness. |
format | Online Article Text |
id | pubmed-8770732 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-87707322022-01-21 Evaluation of Epigenetic Age Based on DNA Methylation Analysis of Several CpG Sites in Ukrainian Population Kuzub, N. Smialkovska, V. Momot, V. Moseiko, V. Lushchak, O. Koliada, A. Front Genet Genetics Epigenetic clocks are the models, which use CpG methylation levels for the age prediction of an organism. Although there were several epigenetic clocks developed there is a demand for development and evaluation of the relatively accurate and sensitive epigenetic clocks that can be used for routine research purposes. In this study, we evaluated two epigenetic clock models based on the 4 CpG sites and 2 CpG sites in the human genome using the pyrosequencing method for their methylation level estimation. The study sample included 153 people from the Ukrainian population with the age from 0 to 101. Both models showed a high correlation with the chronological age in our study sample (R(2) = 0.85 for the 2 CpG model and R(2) = 0.92 for the 4 CpG model). We also estimated the accuracy metrics of the age prediction in our study sample. For the age group from 18 to 80 MAD was 5.1 years for the 2 CpG model and 4.1 years for the 4 CpG model. In this regard, we can conclude, that the models evaluated in the study have good age predictive accuracy, and can be used for the epigenetic age evaluation due to the relative simplicity and time-effectiveness. Frontiers Media S.A. 2022-01-06 /pmc/articles/PMC8770732/ /pubmed/35069680 http://dx.doi.org/10.3389/fgene.2021.772298 Text en Copyright © 2022 Kuzub, Smialkovska, Momot, Moseiko, Lushchak and Koliada. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Kuzub, N. Smialkovska, V. Momot, V. Moseiko, V. Lushchak, O. Koliada, A. Evaluation of Epigenetic Age Based on DNA Methylation Analysis of Several CpG Sites in Ukrainian Population |
title | Evaluation of Epigenetic Age Based on DNA Methylation Analysis of Several CpG Sites in Ukrainian Population |
title_full | Evaluation of Epigenetic Age Based on DNA Methylation Analysis of Several CpG Sites in Ukrainian Population |
title_fullStr | Evaluation of Epigenetic Age Based on DNA Methylation Analysis of Several CpG Sites in Ukrainian Population |
title_full_unstemmed | Evaluation of Epigenetic Age Based on DNA Methylation Analysis of Several CpG Sites in Ukrainian Population |
title_short | Evaluation of Epigenetic Age Based on DNA Methylation Analysis of Several CpG Sites in Ukrainian Population |
title_sort | evaluation of epigenetic age based on dna methylation analysis of several cpg sites in ukrainian population |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8770732/ https://www.ncbi.nlm.nih.gov/pubmed/35069680 http://dx.doi.org/10.3389/fgene.2021.772298 |
work_keys_str_mv | AT kuzubn evaluationofepigeneticagebasedondnamethylationanalysisofseveralcpgsitesinukrainianpopulation AT smialkovskav evaluationofepigeneticagebasedondnamethylationanalysisofseveralcpgsitesinukrainianpopulation AT momotv evaluationofepigeneticagebasedondnamethylationanalysisofseveralcpgsitesinukrainianpopulation AT moseikov evaluationofepigeneticagebasedondnamethylationanalysisofseveralcpgsitesinukrainianpopulation AT lushchako evaluationofepigeneticagebasedondnamethylationanalysisofseveralcpgsitesinukrainianpopulation AT koliadaa evaluationofepigeneticagebasedondnamethylationanalysisofseveralcpgsitesinukrainianpopulation |