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Trophic preferences of the pathogen Ralstonia solanacearum and consequences on its growth in xylem sap

Ralstonia solanacearum is one of the most destructive pathogens worldwide. In the last 30 years, the molecular mechanisms at the origin of R. solanacearum pathogenicity have been studied in depth. However, the nutrition status of the pathogen once inside the plant has been poorly investigated. Yet,...

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Autores principales: Baroukh, Caroline, Zemouri, Meriem, Genin, Stéphane
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8770891/
https://www.ncbi.nlm.nih.gov/pubmed/35212480
http://dx.doi.org/10.1002/mbo3.1240
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author Baroukh, Caroline
Zemouri, Meriem
Genin, Stéphane
author_facet Baroukh, Caroline
Zemouri, Meriem
Genin, Stéphane
author_sort Baroukh, Caroline
collection PubMed
description Ralstonia solanacearum is one of the most destructive pathogens worldwide. In the last 30 years, the molecular mechanisms at the origin of R. solanacearum pathogenicity have been studied in depth. However, the nutrition status of the pathogen once inside the plant has been poorly investigated. Yet, the pathogen needs substrates to sustain a fast‐enough growth, maintain its virulence and subvert the host immunity. This study aimed to explore in‐depth the xylem environment where the pathogen is abundant, and its trophic preferences. First, we determined the composition of tomato xylem sap, where fast multiplication of the pathogen occurs. Then, kinetic growth on single and mixtures of carbon sources in relation to this environment was performed to fully quantify growth. Finally, we calculated the concentration of available metabolites in the xylem sap flux to assess how much it can support bacterial growth in planta. Overall, the study underlines the adaptation of R. solanacearum to the xylem environment and the fact that the pathogen assimilates several substrates at the same time in media composed of several carbon sources. It also provides metrics on key physiological parameters governing the growth of this major pathogen, which will be instrumental in the future to better understand its metabolic behavior during infection.
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spelling pubmed-87708912022-01-24 Trophic preferences of the pathogen Ralstonia solanacearum and consequences on its growth in xylem sap Baroukh, Caroline Zemouri, Meriem Genin, Stéphane Microbiologyopen Original Articles Ralstonia solanacearum is one of the most destructive pathogens worldwide. In the last 30 years, the molecular mechanisms at the origin of R. solanacearum pathogenicity have been studied in depth. However, the nutrition status of the pathogen once inside the plant has been poorly investigated. Yet, the pathogen needs substrates to sustain a fast‐enough growth, maintain its virulence and subvert the host immunity. This study aimed to explore in‐depth the xylem environment where the pathogen is abundant, and its trophic preferences. First, we determined the composition of tomato xylem sap, where fast multiplication of the pathogen occurs. Then, kinetic growth on single and mixtures of carbon sources in relation to this environment was performed to fully quantify growth. Finally, we calculated the concentration of available metabolites in the xylem sap flux to assess how much it can support bacterial growth in planta. Overall, the study underlines the adaptation of R. solanacearum to the xylem environment and the fact that the pathogen assimilates several substrates at the same time in media composed of several carbon sources. It also provides metrics on key physiological parameters governing the growth of this major pathogen, which will be instrumental in the future to better understand its metabolic behavior during infection. John Wiley and Sons Inc. 2022-01-19 /pmc/articles/PMC8770891/ /pubmed/35212480 http://dx.doi.org/10.1002/mbo3.1240 Text en © 2021 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Baroukh, Caroline
Zemouri, Meriem
Genin, Stéphane
Trophic preferences of the pathogen Ralstonia solanacearum and consequences on its growth in xylem sap
title Trophic preferences of the pathogen Ralstonia solanacearum and consequences on its growth in xylem sap
title_full Trophic preferences of the pathogen Ralstonia solanacearum and consequences on its growth in xylem sap
title_fullStr Trophic preferences of the pathogen Ralstonia solanacearum and consequences on its growth in xylem sap
title_full_unstemmed Trophic preferences of the pathogen Ralstonia solanacearum and consequences on its growth in xylem sap
title_short Trophic preferences of the pathogen Ralstonia solanacearum and consequences on its growth in xylem sap
title_sort trophic preferences of the pathogen ralstonia solanacearum and consequences on its growth in xylem sap
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8770891/
https://www.ncbi.nlm.nih.gov/pubmed/35212480
http://dx.doi.org/10.1002/mbo3.1240
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