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Bacterial Detection and Recovery From Poultry Litter

The level of pathogens in poultry litter used for raising broiler chickens is critical to the overall health of a broiler chicken flock and food safety. Therefore, it is imperative that methods used for determining bacterial concentration in litter are accurate and reproducible across studies. In th...

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Autores principales: Plumblee Lawrence, Jodie R., Cudnik, Denice, Oladeinde, Adelumola
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8770916/
https://www.ncbi.nlm.nih.gov/pubmed/35069507
http://dx.doi.org/10.3389/fmicb.2021.803150
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author Plumblee Lawrence, Jodie R.
Cudnik, Denice
Oladeinde, Adelumola
author_facet Plumblee Lawrence, Jodie R.
Cudnik, Denice
Oladeinde, Adelumola
author_sort Plumblee Lawrence, Jodie R.
collection PubMed
description The level of pathogens in poultry litter used for raising broiler chickens is critical to the overall health of a broiler chicken flock and food safety. Therefore, it is imperative that methods used for determining bacterial concentration in litter are accurate and reproducible across studies. In this perspective, we discuss the shortcomings associated with current methods used for bacterial quantification and detection from litter and assess the efficacy of one method for pathogen and commensal (Campylobacter, Salmonella, Escherichia coli, and Enterococcus spp.) recovery. The limit of quantitation and detection for this method differed between pathogens, and the recovery rate (∼138–208%) was higher for Salmonella, E. coli, and Enterococcus compared to Campylobacter (24%). Our results suggest that pathogen recovery from litter is highly variable and pathogen concentrations need to be reported in dry weight before comparisons can be made between studies.
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spelling pubmed-87709162022-01-21 Bacterial Detection and Recovery From Poultry Litter Plumblee Lawrence, Jodie R. Cudnik, Denice Oladeinde, Adelumola Front Microbiol Microbiology The level of pathogens in poultry litter used for raising broiler chickens is critical to the overall health of a broiler chicken flock and food safety. Therefore, it is imperative that methods used for determining bacterial concentration in litter are accurate and reproducible across studies. In this perspective, we discuss the shortcomings associated with current methods used for bacterial quantification and detection from litter and assess the efficacy of one method for pathogen and commensal (Campylobacter, Salmonella, Escherichia coli, and Enterococcus spp.) recovery. The limit of quantitation and detection for this method differed between pathogens, and the recovery rate (∼138–208%) was higher for Salmonella, E. coli, and Enterococcus compared to Campylobacter (24%). Our results suggest that pathogen recovery from litter is highly variable and pathogen concentrations need to be reported in dry weight before comparisons can be made between studies. Frontiers Media S.A. 2022-01-06 /pmc/articles/PMC8770916/ /pubmed/35069507 http://dx.doi.org/10.3389/fmicb.2021.803150 Text en Copyright © 2022 Plumblee Lawrence, Cudnik and Oladeinde. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Plumblee Lawrence, Jodie R.
Cudnik, Denice
Oladeinde, Adelumola
Bacterial Detection and Recovery From Poultry Litter
title Bacterial Detection and Recovery From Poultry Litter
title_full Bacterial Detection and Recovery From Poultry Litter
title_fullStr Bacterial Detection and Recovery From Poultry Litter
title_full_unstemmed Bacterial Detection and Recovery From Poultry Litter
title_short Bacterial Detection and Recovery From Poultry Litter
title_sort bacterial detection and recovery from poultry litter
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8770916/
https://www.ncbi.nlm.nih.gov/pubmed/35069507
http://dx.doi.org/10.3389/fmicb.2021.803150
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