Cargando…

SK Current, Expressed During the Development and Regeneration of Chick Hair Cells, Contributes to the Patterning of Spontaneous Action Potentials

Chick hair cells display calcium (Ca(2+))-sensitive spontaneous action potentials during development and regeneration. The role of this activity is unclear but thought to be involved in establishing proper synaptic connections and tonotopic maps, both of which are instrumental to normal hearing. Usi...

Descripción completa

Detalles Bibliográficos
Autor principal: Levic, Snezana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8770932/
https://www.ncbi.nlm.nih.gov/pubmed/35069114
http://dx.doi.org/10.3389/fncel.2021.766264
_version_ 1784635483159003136
author Levic, Snezana
author_facet Levic, Snezana
author_sort Levic, Snezana
collection PubMed
description Chick hair cells display calcium (Ca(2+))-sensitive spontaneous action potentials during development and regeneration. The role of this activity is unclear but thought to be involved in establishing proper synaptic connections and tonotopic maps, both of which are instrumental to normal hearing. Using an electrophysiological approach, this work investigated the functional expression of Ca(2+)-sensitive potassium [I(K(Ca))] currents and their role in spontaneous electrical activity in the developing and regenerating hair cells (HCs) in the chick basilar papilla. The main I(K(Ca)) in developing and regenerating chick HCs is an SK current, based on its sensitivity to apamin. Analysis of the functional expression of SK current showed that most dramatic changes occurred between E8 and E16. Specifically, there is a developmental downregulation of the SK current after E16. The SK current gating was very sensitive to the availability of intracellular Ca(2+) but showed very little sensitivity to T-type voltage-gated Ca(2+) channels, which are one of the hallmarks of developing and regenerating hair cells. Additionally, apamin reduced the frequency of spontaneous electrical activity in HCs, suggesting that SK current participates in patterning the spontaneous electrical activity of HCs.
format Online
Article
Text
id pubmed-8770932
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-87709322022-01-21 SK Current, Expressed During the Development and Regeneration of Chick Hair Cells, Contributes to the Patterning of Spontaneous Action Potentials Levic, Snezana Front Cell Neurosci Neuroscience Chick hair cells display calcium (Ca(2+))-sensitive spontaneous action potentials during development and regeneration. The role of this activity is unclear but thought to be involved in establishing proper synaptic connections and tonotopic maps, both of which are instrumental to normal hearing. Using an electrophysiological approach, this work investigated the functional expression of Ca(2+)-sensitive potassium [I(K(Ca))] currents and their role in spontaneous electrical activity in the developing and regenerating hair cells (HCs) in the chick basilar papilla. The main I(K(Ca)) in developing and regenerating chick HCs is an SK current, based on its sensitivity to apamin. Analysis of the functional expression of SK current showed that most dramatic changes occurred between E8 and E16. Specifically, there is a developmental downregulation of the SK current after E16. The SK current gating was very sensitive to the availability of intracellular Ca(2+) but showed very little sensitivity to T-type voltage-gated Ca(2+) channels, which are one of the hallmarks of developing and regenerating hair cells. Additionally, apamin reduced the frequency of spontaneous electrical activity in HCs, suggesting that SK current participates in patterning the spontaneous electrical activity of HCs. Frontiers Media S.A. 2022-01-06 /pmc/articles/PMC8770932/ /pubmed/35069114 http://dx.doi.org/10.3389/fncel.2021.766264 Text en Copyright © 2022 Levic. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Levic, Snezana
SK Current, Expressed During the Development and Regeneration of Chick Hair Cells, Contributes to the Patterning of Spontaneous Action Potentials
title SK Current, Expressed During the Development and Regeneration of Chick Hair Cells, Contributes to the Patterning of Spontaneous Action Potentials
title_full SK Current, Expressed During the Development and Regeneration of Chick Hair Cells, Contributes to the Patterning of Spontaneous Action Potentials
title_fullStr SK Current, Expressed During the Development and Regeneration of Chick Hair Cells, Contributes to the Patterning of Spontaneous Action Potentials
title_full_unstemmed SK Current, Expressed During the Development and Regeneration of Chick Hair Cells, Contributes to the Patterning of Spontaneous Action Potentials
title_short SK Current, Expressed During the Development and Regeneration of Chick Hair Cells, Contributes to the Patterning of Spontaneous Action Potentials
title_sort sk current, expressed during the development and regeneration of chick hair cells, contributes to the patterning of spontaneous action potentials
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8770932/
https://www.ncbi.nlm.nih.gov/pubmed/35069114
http://dx.doi.org/10.3389/fncel.2021.766264
work_keys_str_mv AT levicsnezana skcurrentexpressedduringthedevelopmentandregenerationofchickhaircellscontributestothepatterningofspontaneousactionpotentials