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Rapid electrochemical detection of COVID-19 genomic sequence with dual-function graphene nanocolloids based biosensor

Discovered in December 2019, the Severe Acute Respiratory Syndrome Coronavirus 2 (aka SARS-CoV-2 or 2019-nCoV) has attracted worldwide attention and concerns due to its high transmissibility and the severe health consequences experienced upon its infection, particularly by elderly people. Over 329 m...

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Autores principales: Ang, Wei Li, Lim, Rachel Rui Xia, Ambrosi, Adriano, Bonanni, Alessandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8771053/
http://dx.doi.org/10.1016/j.flatc.2022.100336
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author Ang, Wei Li
Lim, Rachel Rui Xia
Ambrosi, Adriano
Bonanni, Alessandra
author_facet Ang, Wei Li
Lim, Rachel Rui Xia
Ambrosi, Adriano
Bonanni, Alessandra
author_sort Ang, Wei Li
collection PubMed
description Discovered in December 2019, the Severe Acute Respiratory Syndrome Coronavirus 2 (aka SARS-CoV-2 or 2019-nCoV) has attracted worldwide attention and concerns due to its high transmissibility and the severe health consequences experienced upon its infection, particularly by elderly people. Over 329 million people have been infected till date and over 5.5 million people could not survive the respiratory illness known as COVID-19 syndrome. Rapid and low-cost detection methods are of utmost importance to monitor the diffusion of the virus and to aid in the global fight against the pandemic. We propose here the use of graphene oxide nanocolloids (GONC) as an electroactive nanocarbon material that can act simultaneously as a transducing platform as well as the electroactive label for the detection of 2019-nCoV genomic sequences. The ability of GONC to provide an intrinsic electrochemical signal arising from the reduction of the electrochemically reducible oxygen functionalities present on its surface, allows GONC to be used as a simple and sensitive biosensing platform. Different intrinsic electroactivity of the material was obtained at each step of the genosensing process, starting from the immobilization of a short-stranded DNA probe and followed by the incubation with different concentrations of the target 2019-nCoV DNA strand. Monitoring such variations enabled the quantification of the target analyte over a wide dynamic range between 10(−10) and 10(−5) M. All in all, this proof-of-concept system serves as a stepping stone for the development of a rapid, sensitive and selective analytical tool for the detection of 2019-nCoV as well as other similar viral vectors. The use of cost-effective electrochemical detection methods coupled with the vast availability and suitability of carbon-based nanomaterials make this sensing system a valid candidate for low-cost and point-of-care analysis.
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spelling pubmed-87710532022-01-20 Rapid electrochemical detection of COVID-19 genomic sequence with dual-function graphene nanocolloids based biosensor Ang, Wei Li Lim, Rachel Rui Xia Ambrosi, Adriano Bonanni, Alessandra FlatChem Article Discovered in December 2019, the Severe Acute Respiratory Syndrome Coronavirus 2 (aka SARS-CoV-2 or 2019-nCoV) has attracted worldwide attention and concerns due to its high transmissibility and the severe health consequences experienced upon its infection, particularly by elderly people. Over 329 million people have been infected till date and over 5.5 million people could not survive the respiratory illness known as COVID-19 syndrome. Rapid and low-cost detection methods are of utmost importance to monitor the diffusion of the virus and to aid in the global fight against the pandemic. We propose here the use of graphene oxide nanocolloids (GONC) as an electroactive nanocarbon material that can act simultaneously as a transducing platform as well as the electroactive label for the detection of 2019-nCoV genomic sequences. The ability of GONC to provide an intrinsic electrochemical signal arising from the reduction of the electrochemically reducible oxygen functionalities present on its surface, allows GONC to be used as a simple and sensitive biosensing platform. Different intrinsic electroactivity of the material was obtained at each step of the genosensing process, starting from the immobilization of a short-stranded DNA probe and followed by the incubation with different concentrations of the target 2019-nCoV DNA strand. Monitoring such variations enabled the quantification of the target analyte over a wide dynamic range between 10(−10) and 10(−5) M. All in all, this proof-of-concept system serves as a stepping stone for the development of a rapid, sensitive and selective analytical tool for the detection of 2019-nCoV as well as other similar viral vectors. The use of cost-effective electrochemical detection methods coupled with the vast availability and suitability of carbon-based nanomaterials make this sensing system a valid candidate for low-cost and point-of-care analysis. Elsevier B.V. 2022-03 2022-01-15 /pmc/articles/PMC8771053/ http://dx.doi.org/10.1016/j.flatc.2022.100336 Text en © 2022 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Ang, Wei Li
Lim, Rachel Rui Xia
Ambrosi, Adriano
Bonanni, Alessandra
Rapid electrochemical detection of COVID-19 genomic sequence with dual-function graphene nanocolloids based biosensor
title Rapid electrochemical detection of COVID-19 genomic sequence with dual-function graphene nanocolloids based biosensor
title_full Rapid electrochemical detection of COVID-19 genomic sequence with dual-function graphene nanocolloids based biosensor
title_fullStr Rapid electrochemical detection of COVID-19 genomic sequence with dual-function graphene nanocolloids based biosensor
title_full_unstemmed Rapid electrochemical detection of COVID-19 genomic sequence with dual-function graphene nanocolloids based biosensor
title_short Rapid electrochemical detection of COVID-19 genomic sequence with dual-function graphene nanocolloids based biosensor
title_sort rapid electrochemical detection of covid-19 genomic sequence with dual-function graphene nanocolloids based biosensor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8771053/
http://dx.doi.org/10.1016/j.flatc.2022.100336
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