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An Automated Canine Line-Up for Detection Dog Research

Currently, there is a need to develop technology that facilitates and improves detection dog research. The aim of this research was to develop an automated computer-driven olfactory line-up task. The apparatus consisted of three olfactometers. Each olfactometer was equipped with flow meters to regul...

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Autores principales: Aviles-Rosa, Edgar O., Gallegos, Shawna F., Prada-Tiedemann, Paola A., Hall, Nathaniel J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8771161/
https://www.ncbi.nlm.nih.gov/pubmed/35071382
http://dx.doi.org/10.3389/fvets.2021.775381
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author Aviles-Rosa, Edgar O.
Gallegos, Shawna F.
Prada-Tiedemann, Paola A.
Hall, Nathaniel J.
author_facet Aviles-Rosa, Edgar O.
Gallegos, Shawna F.
Prada-Tiedemann, Paola A.
Hall, Nathaniel J.
author_sort Aviles-Rosa, Edgar O.
collection PubMed
description Currently, there is a need to develop technology that facilitates and improves detection dog research. The aim of this research was to develop an automated computer-driven olfactory line-up task. The apparatus consisted of three olfactometers. Each olfactometer was equipped with flow meters to regulate air flow and dilution and six solenoid valves connected to odor jars. Each olfactometer generated an odor which was carried to an odor port where the dogs sample it. The olfactometer's valves were activated by a microcontroller, and a Python program was built to control each olfactometer and randomize and balance the odor presentation. Dogs (N = 12) received one or two 40-trial training sessions in a day where they progressed through a series of training phases where they learned to detect and alert to double-base smokeless powder (SP). An “alert” consisted of a 4-s nose hold. This was measured by infrared sensors in the ports. For each trial, the apparatus recorded dogs' search latency, sniff time, port entries, and response. All this information was automatically recorded in a csv file. A photoionization detector (PID) and solid-phase microextraction followed by gas chromatography-mass spectrometry (SPME-GC/MS) were used to evaluate the odor dynamics and to instrumentally verify odor presence and clearance. A control test was conducted at the end of the training to ensure dogs were alerting exclusively to the odorant. All 12 dogs readily learned to operate the apparatus within 23 days, and all exceeded 85% accuracy. Control tests indicated dogs were leveraging only olfactory cues and not unintentional cues such as auditory cues from the apparatus. Analytical data showed that odor was detected in the port immediately after the activation of a valve and that odor clearance occurred immediately after the valve was closed. The apparatus developed was easy to operate by the dogs and allowed substantial data collection using double-blind testing procedures in a very short period at an affordable cost point for research equipment (~$5,000 USD). The apparatus may prove to be a useful research tool to provide optimal odor stimuli control, ensure double-blind conditions, reduce labor, and significantly increase the amount of data collected.
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spelling pubmed-87711612022-01-21 An Automated Canine Line-Up for Detection Dog Research Aviles-Rosa, Edgar O. Gallegos, Shawna F. Prada-Tiedemann, Paola A. Hall, Nathaniel J. Front Vet Sci Veterinary Science Currently, there is a need to develop technology that facilitates and improves detection dog research. The aim of this research was to develop an automated computer-driven olfactory line-up task. The apparatus consisted of three olfactometers. Each olfactometer was equipped with flow meters to regulate air flow and dilution and six solenoid valves connected to odor jars. Each olfactometer generated an odor which was carried to an odor port where the dogs sample it. The olfactometer's valves were activated by a microcontroller, and a Python program was built to control each olfactometer and randomize and balance the odor presentation. Dogs (N = 12) received one or two 40-trial training sessions in a day where they progressed through a series of training phases where they learned to detect and alert to double-base smokeless powder (SP). An “alert” consisted of a 4-s nose hold. This was measured by infrared sensors in the ports. For each trial, the apparatus recorded dogs' search latency, sniff time, port entries, and response. All this information was automatically recorded in a csv file. A photoionization detector (PID) and solid-phase microextraction followed by gas chromatography-mass spectrometry (SPME-GC/MS) were used to evaluate the odor dynamics and to instrumentally verify odor presence and clearance. A control test was conducted at the end of the training to ensure dogs were alerting exclusively to the odorant. All 12 dogs readily learned to operate the apparatus within 23 days, and all exceeded 85% accuracy. Control tests indicated dogs were leveraging only olfactory cues and not unintentional cues such as auditory cues from the apparatus. Analytical data showed that odor was detected in the port immediately after the activation of a valve and that odor clearance occurred immediately after the valve was closed. The apparatus developed was easy to operate by the dogs and allowed substantial data collection using double-blind testing procedures in a very short period at an affordable cost point for research equipment (~$5,000 USD). The apparatus may prove to be a useful research tool to provide optimal odor stimuli control, ensure double-blind conditions, reduce labor, and significantly increase the amount of data collected. Frontiers Media S.A. 2021-12-20 /pmc/articles/PMC8771161/ /pubmed/35071382 http://dx.doi.org/10.3389/fvets.2021.775381 Text en Copyright © 2021 Aviles-Rosa, Gallegos, Prada-Tiedemann and Hall. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Aviles-Rosa, Edgar O.
Gallegos, Shawna F.
Prada-Tiedemann, Paola A.
Hall, Nathaniel J.
An Automated Canine Line-Up for Detection Dog Research
title An Automated Canine Line-Up for Detection Dog Research
title_full An Automated Canine Line-Up for Detection Dog Research
title_fullStr An Automated Canine Line-Up for Detection Dog Research
title_full_unstemmed An Automated Canine Line-Up for Detection Dog Research
title_short An Automated Canine Line-Up for Detection Dog Research
title_sort automated canine line-up for detection dog research
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8771161/
https://www.ncbi.nlm.nih.gov/pubmed/35071382
http://dx.doi.org/10.3389/fvets.2021.775381
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