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Promoter Methylation-Mediated NPTX2 Silencing Promotes Tumor Growth in Human Prostate Cancer

Neuronal pentraxin 2 (NPTX2), a secretory protein of neuronal pentraxins, was first identified in the nervous system. Several studies have shown that expression levels of NPTX2 are associated with the development of various cancers. However, whether NPTX2 is involved in prostate cancer progression i...

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Autores principales: Su, Yixi, Huang, Qiang, Lu, Li, Qu, Hu, Wang, Dejuan, Qiu, Jianguang, Li, Weiqian, Lin, Mengmeng, Liu, Huanliang, Wang, Zhongyang, Yang, Xiangling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8771508/
https://www.ncbi.nlm.nih.gov/pubmed/35069913
http://dx.doi.org/10.7150/jca.65214
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author Su, Yixi
Huang, Qiang
Lu, Li
Qu, Hu
Wang, Dejuan
Qiu, Jianguang
Li, Weiqian
Lin, Mengmeng
Liu, Huanliang
Wang, Zhongyang
Yang, Xiangling
author_facet Su, Yixi
Huang, Qiang
Lu, Li
Qu, Hu
Wang, Dejuan
Qiu, Jianguang
Li, Weiqian
Lin, Mengmeng
Liu, Huanliang
Wang, Zhongyang
Yang, Xiangling
author_sort Su, Yixi
collection PubMed
description Neuronal pentraxin 2 (NPTX2), a secretory protein of neuronal pentraxins, was first identified in the nervous system. Several studies have shown that expression levels of NPTX2 are associated with the development of various cancers. However, whether NPTX2 is involved in prostate cancer progression is unclear. Herein, we found that NPTX2 is significantly reduced in prostate cancer tissues and cancer cell lines compared to control prostate tissues and control prostatic epithelial cell lines. Furthermore, the NPTX2 promoter is highly methylated in prostate cancer cells. Consistently, NPTX2 could be restored by treatment with the DNA methyltransferase inhibitor 5-aza-2′-deoxycytidine (decitabine, 5-AZA-dC). Overexpression of NPTX2 inhibited prostate cancer cell proliferation both in vitro and in vivo. In conclusion, our study demonstrated that NPTX2 acts as a tumor suppressor gene in prostate cancer.
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spelling pubmed-87715082022-01-20 Promoter Methylation-Mediated NPTX2 Silencing Promotes Tumor Growth in Human Prostate Cancer Su, Yixi Huang, Qiang Lu, Li Qu, Hu Wang, Dejuan Qiu, Jianguang Li, Weiqian Lin, Mengmeng Liu, Huanliang Wang, Zhongyang Yang, Xiangling J Cancer Research Paper Neuronal pentraxin 2 (NPTX2), a secretory protein of neuronal pentraxins, was first identified in the nervous system. Several studies have shown that expression levels of NPTX2 are associated with the development of various cancers. However, whether NPTX2 is involved in prostate cancer progression is unclear. Herein, we found that NPTX2 is significantly reduced in prostate cancer tissues and cancer cell lines compared to control prostate tissues and control prostatic epithelial cell lines. Furthermore, the NPTX2 promoter is highly methylated in prostate cancer cells. Consistently, NPTX2 could be restored by treatment with the DNA methyltransferase inhibitor 5-aza-2′-deoxycytidine (decitabine, 5-AZA-dC). Overexpression of NPTX2 inhibited prostate cancer cell proliferation both in vitro and in vivo. In conclusion, our study demonstrated that NPTX2 acts as a tumor suppressor gene in prostate cancer. Ivyspring International Publisher 2022-01-01 /pmc/articles/PMC8771508/ /pubmed/35069913 http://dx.doi.org/10.7150/jca.65214 Text en © The author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Su, Yixi
Huang, Qiang
Lu, Li
Qu, Hu
Wang, Dejuan
Qiu, Jianguang
Li, Weiqian
Lin, Mengmeng
Liu, Huanliang
Wang, Zhongyang
Yang, Xiangling
Promoter Methylation-Mediated NPTX2 Silencing Promotes Tumor Growth in Human Prostate Cancer
title Promoter Methylation-Mediated NPTX2 Silencing Promotes Tumor Growth in Human Prostate Cancer
title_full Promoter Methylation-Mediated NPTX2 Silencing Promotes Tumor Growth in Human Prostate Cancer
title_fullStr Promoter Methylation-Mediated NPTX2 Silencing Promotes Tumor Growth in Human Prostate Cancer
title_full_unstemmed Promoter Methylation-Mediated NPTX2 Silencing Promotes Tumor Growth in Human Prostate Cancer
title_short Promoter Methylation-Mediated NPTX2 Silencing Promotes Tumor Growth in Human Prostate Cancer
title_sort promoter methylation-mediated nptx2 silencing promotes tumor growth in human prostate cancer
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8771508/
https://www.ncbi.nlm.nih.gov/pubmed/35069913
http://dx.doi.org/10.7150/jca.65214
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