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Isoenergetic-practical and semi-purified diets for protein requirement determination in Hermetia illucens larvae: consequences on life history traits

BACKGROUND: Black soldier fly (BSF) is one of the most promising species for the intensive breeding of insects given its adaptability and its efficiency in the conversion of waste. To maximize the production and use waste as substrates, it is essential to determine the larvae nutritional requirement...

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Autores principales: Bellezza Oddon, Sara, Biasato, Ilaria, Gasco, Laura
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8772230/
https://www.ncbi.nlm.nih.gov/pubmed/35057856
http://dx.doi.org/10.1186/s40104-021-00659-y
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author Bellezza Oddon, Sara
Biasato, Ilaria
Gasco, Laura
author_facet Bellezza Oddon, Sara
Biasato, Ilaria
Gasco, Laura
author_sort Bellezza Oddon, Sara
collection PubMed
description BACKGROUND: Black soldier fly (BSF) is one of the most promising species for the intensive breeding of insects given its adaptability and its efficiency in the conversion of waste. To maximize the production and use waste as substrates, it is essential to determine the larvae nutritional requirements. The study aims to evaluate the effects of 5 practical, semi-purified and isoenergetic diets (PSPID) with increasing protein levels (10%, CP10; 14%, CP14; 16%, CP16; 19%, CP19) on BSF life history traits. A total of 2000 six-day-old larvae were weighed and divided into groups of 100 (4 replicates/treatment [PSPID and Gainesville diet (GA) used as environmental control]). In larva-prepupa stage, sampling was carried out on 30 individuals/replicate, while in prepupa-adult stage on 35. RESULTS: In the whole larval stage, the CP16 treatment performed better when compared to the other groups. At 18 days old, the CP14 treatment showed a higher weight than the CP19 (P < 0.01), while the CP10 and CP16 weights were intermediate. On the contrary, the CP10 prepupae weight was higher than the CP19 (P < 0.01). The CP10 and CP14 pupae performed better in terms of weight when compared to the others (P < 0.01). The dietary treatments did not affect the adult parameters. The sex significantly influenced both the exuvia weight, which was the greatest in females (P < 0.001), and the fly lifespan, longest in males (P < 0.05). Fly lifespan was also influenced by the interaction between treatment and sex, with the CP19 females showing a longer life than the others (P < 0.05). CONCLUSIONS: In conclusion, the optimal protein level considering the whole larval stage is 16% on dry matter (DM). At 18 days old, looking at the size intended for the meal production, it can be considered 14% on DM. The result obtained on adult emergence in PSPID may not be attributable to the protein content. Further researches on macronutrients requirements determination have to be conducted to evaluate the substrates composition effects on BSF life history traits.
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spelling pubmed-87722302022-01-20 Isoenergetic-practical and semi-purified diets for protein requirement determination in Hermetia illucens larvae: consequences on life history traits Bellezza Oddon, Sara Biasato, Ilaria Gasco, Laura J Anim Sci Biotechnol Research BACKGROUND: Black soldier fly (BSF) is one of the most promising species for the intensive breeding of insects given its adaptability and its efficiency in the conversion of waste. To maximize the production and use waste as substrates, it is essential to determine the larvae nutritional requirements. The study aims to evaluate the effects of 5 practical, semi-purified and isoenergetic diets (PSPID) with increasing protein levels (10%, CP10; 14%, CP14; 16%, CP16; 19%, CP19) on BSF life history traits. A total of 2000 six-day-old larvae were weighed and divided into groups of 100 (4 replicates/treatment [PSPID and Gainesville diet (GA) used as environmental control]). In larva-prepupa stage, sampling was carried out on 30 individuals/replicate, while in prepupa-adult stage on 35. RESULTS: In the whole larval stage, the CP16 treatment performed better when compared to the other groups. At 18 days old, the CP14 treatment showed a higher weight than the CP19 (P < 0.01), while the CP10 and CP16 weights were intermediate. On the contrary, the CP10 prepupae weight was higher than the CP19 (P < 0.01). The CP10 and CP14 pupae performed better in terms of weight when compared to the others (P < 0.01). The dietary treatments did not affect the adult parameters. The sex significantly influenced both the exuvia weight, which was the greatest in females (P < 0.001), and the fly lifespan, longest in males (P < 0.05). Fly lifespan was also influenced by the interaction between treatment and sex, with the CP19 females showing a longer life than the others (P < 0.05). CONCLUSIONS: In conclusion, the optimal protein level considering the whole larval stage is 16% on dry matter (DM). At 18 days old, looking at the size intended for the meal production, it can be considered 14% on DM. The result obtained on adult emergence in PSPID may not be attributable to the protein content. Further researches on macronutrients requirements determination have to be conducted to evaluate the substrates composition effects on BSF life history traits. BioMed Central 2022-01-19 /pmc/articles/PMC8772230/ /pubmed/35057856 http://dx.doi.org/10.1186/s40104-021-00659-y Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Bellezza Oddon, Sara
Biasato, Ilaria
Gasco, Laura
Isoenergetic-practical and semi-purified diets for protein requirement determination in Hermetia illucens larvae: consequences on life history traits
title Isoenergetic-practical and semi-purified diets for protein requirement determination in Hermetia illucens larvae: consequences on life history traits
title_full Isoenergetic-practical and semi-purified diets for protein requirement determination in Hermetia illucens larvae: consequences on life history traits
title_fullStr Isoenergetic-practical and semi-purified diets for protein requirement determination in Hermetia illucens larvae: consequences on life history traits
title_full_unstemmed Isoenergetic-practical and semi-purified diets for protein requirement determination in Hermetia illucens larvae: consequences on life history traits
title_short Isoenergetic-practical and semi-purified diets for protein requirement determination in Hermetia illucens larvae: consequences on life history traits
title_sort isoenergetic-practical and semi-purified diets for protein requirement determination in hermetia illucens larvae: consequences on life history traits
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8772230/
https://www.ncbi.nlm.nih.gov/pubmed/35057856
http://dx.doi.org/10.1186/s40104-021-00659-y
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