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In Vitro and In Vivo Fluorescence Imaging of Antibody–Drug Conjugate-Induced Tumor Apoptosis Using Annexin V–EGFP Conjugated Quantum Dots
[Image: see text] Antibody–drug conjugates (ADCs) are conjugates of a monoclonal antibody and a cytotoxic drug that induce tumor apoptosis. The evaluation of ADC-induced tumor apoptosis is crucial for the development of ADCs for cancer therapy. To evaluate the efficacy of ADCs, we present in vitro a...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8772308/ https://www.ncbi.nlm.nih.gov/pubmed/35071899 http://dx.doi.org/10.1021/acsomega.1c05636 |
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author | Tsuboi, Setsuko Jin, Takashi |
author_facet | Tsuboi, Setsuko Jin, Takashi |
author_sort | Tsuboi, Setsuko |
collection | PubMed |
description | [Image: see text] Antibody–drug conjugates (ADCs) are conjugates of a monoclonal antibody and a cytotoxic drug that induce tumor apoptosis. The evaluation of ADC-induced tumor apoptosis is crucial for the development of ADCs for cancer therapy. To evaluate the efficacy of ADCs, we present in vitro and in vivo fluorescence imaging techniques for ADC-induced tumor apoptosis using annexin V–EGFP (EGFP: enhanced green fluorescent protein) conjugated quantum dots (annexin V–EGFP–QDs). This probe emits visible (VIS) and near-infrared (NIR) dual fluorescence at 515 nm (EGFP emission) and 850 nm (QD emission), which can be used for the detection of tumor apoptosis at the cellular and whole-body levels. By using annexin V–EGFP–QDs, we achieved VIS and NIR fluorescence imaging of human epidermal growth factor receptor 2-positive breast tumor apoptosis induced by an ADC, Kadcyla (trastuzumab emtansine). The results show that the in vitro and in vivo fluorescence imaging of ADC-induced tumor apoptosis using annexin V–EGFP–QDs is a useful tool to evaluate the efficacy of ADCs for cancer therapy. |
format | Online Article Text |
id | pubmed-8772308 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-87723082022-01-21 In Vitro and In Vivo Fluorescence Imaging of Antibody–Drug Conjugate-Induced Tumor Apoptosis Using Annexin V–EGFP Conjugated Quantum Dots Tsuboi, Setsuko Jin, Takashi ACS Omega [Image: see text] Antibody–drug conjugates (ADCs) are conjugates of a monoclonal antibody and a cytotoxic drug that induce tumor apoptosis. The evaluation of ADC-induced tumor apoptosis is crucial for the development of ADCs for cancer therapy. To evaluate the efficacy of ADCs, we present in vitro and in vivo fluorescence imaging techniques for ADC-induced tumor apoptosis using annexin V–EGFP (EGFP: enhanced green fluorescent protein) conjugated quantum dots (annexin V–EGFP–QDs). This probe emits visible (VIS) and near-infrared (NIR) dual fluorescence at 515 nm (EGFP emission) and 850 nm (QD emission), which can be used for the detection of tumor apoptosis at the cellular and whole-body levels. By using annexin V–EGFP–QDs, we achieved VIS and NIR fluorescence imaging of human epidermal growth factor receptor 2-positive breast tumor apoptosis induced by an ADC, Kadcyla (trastuzumab emtansine). The results show that the in vitro and in vivo fluorescence imaging of ADC-induced tumor apoptosis using annexin V–EGFP–QDs is a useful tool to evaluate the efficacy of ADCs for cancer therapy. American Chemical Society 2022-01-03 /pmc/articles/PMC8772308/ /pubmed/35071899 http://dx.doi.org/10.1021/acsomega.1c05636 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Tsuboi, Setsuko Jin, Takashi In Vitro and In Vivo Fluorescence Imaging of Antibody–Drug Conjugate-Induced Tumor Apoptosis Using Annexin V–EGFP Conjugated Quantum Dots |
title | In Vitro and In Vivo Fluorescence Imaging of Antibody–Drug
Conjugate-Induced Tumor Apoptosis Using Annexin V–EGFP Conjugated
Quantum Dots |
title_full | In Vitro and In Vivo Fluorescence Imaging of Antibody–Drug
Conjugate-Induced Tumor Apoptosis Using Annexin V–EGFP Conjugated
Quantum Dots |
title_fullStr | In Vitro and In Vivo Fluorescence Imaging of Antibody–Drug
Conjugate-Induced Tumor Apoptosis Using Annexin V–EGFP Conjugated
Quantum Dots |
title_full_unstemmed | In Vitro and In Vivo Fluorescence Imaging of Antibody–Drug
Conjugate-Induced Tumor Apoptosis Using Annexin V–EGFP Conjugated
Quantum Dots |
title_short | In Vitro and In Vivo Fluorescence Imaging of Antibody–Drug
Conjugate-Induced Tumor Apoptosis Using Annexin V–EGFP Conjugated
Quantum Dots |
title_sort | in vitro and in vivo fluorescence imaging of antibody–drug
conjugate-induced tumor apoptosis using annexin v–egfp conjugated
quantum dots |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8772308/ https://www.ncbi.nlm.nih.gov/pubmed/35071899 http://dx.doi.org/10.1021/acsomega.1c05636 |
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