Productive Performance and Cecum Microbiota Analysis of Broiler Chickens Supplemented with β-Mannanases and Bacteriophages—A Pilot Study

SIMPLE SUMMARY: For several years, antibiotic growth promoters (AGPs) have been used in poultry production; however, with the recent ban on the use of AGPs, several alternatives have been evaluated. In the present work, we evaluated the use of β-mannanases and bacteriophages as an alternative to AGPs. This study demonstrates that supplementation with β-mannanases, bacteriophages, or a mix of these two does not affect the productive performance in broilers fed corn–soybean meal. The mixture of β-mannanases and bacteriophages promoted the abundance of beneficial microorganisms in the cecum. These preliminary results suggest that β-mannanases and bacteriophages have potential as alternatives to AGPs in poultry production. ABSTRACT: This study was conducted to evaluate the productive performance, intestinal health, and description of the cecum microbiota in broilers supplemented with β-mannanases (MNs) and bacteriophages (BPs). Six hundred one-day-old broilers were divided into four groups and fed one of the following diets: CON—corn–soybean meal + 10 ppm enramycin (ENR); MN: CON + 500 ppm MN; BP: CON + 500 ppm BP; MN + BP: BP + 500 ppm MN. The BP and MN factors showed similar performances to ENR. MN improved the concentration of IgA in the jejunum at 35 days of age. The morphometric index (IM) of the thymus increased by adding MN, while BP increased the liver and thymus IM. The histological analysis showed that BP and MN improved the intestinal morphology. MN + BP showed a tendency to decrease the abundance of Proteobacteria and increase the abundance of Bacteroidetes, indicating better microbiota function. In conclusion, our results demonstrate that the combination of MN + BP has potential in poultry nutrition; however, we highly recommend further experiments to confirm this hypothesis.