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(51)Cr-EDTA plasma clearance in children: One, two, or multiple samples?

Plasma disappearance curves using multiple blood samples are a recognized reference method for measuring glomerular filtration rate (GFR). However, there is no consensus on the protocol for this type of measurement. A two-compartment model is generally considered acceptable for the mathematical desc...

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Autores principales: Pottel, Hans, Goffin, Karolien, De Waele, Liesbeth, Levtchenko, Elena, Delanaye, Pierre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Lippincott Williams & Wilkins 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8772627/
https://www.ncbi.nlm.nih.gov/pubmed/35060531
http://dx.doi.org/10.1097/MD.0000000000028608
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author Pottel, Hans
Goffin, Karolien
De Waele, Liesbeth
Levtchenko, Elena
Delanaye, Pierre
author_facet Pottel, Hans
Goffin, Karolien
De Waele, Liesbeth
Levtchenko, Elena
Delanaye, Pierre
author_sort Pottel, Hans
collection PubMed
description Plasma disappearance curves using multiple blood samples are a recognized reference method for measuring glomerular filtration rate (GFR). However, there is no consensus on the protocol for this type of measurement. A two-compartment model is generally considered acceptable for the mathematical description of the concentration–time decay curve. The impact of the fitting procedure on the reported GFR has not been questioned. We defined 8 different fitting procedures to calculate the area under the curve, and from this area under the curve, the GFR. We applied the 8 fitting methods (all considering a full concentration–time curve) on the multiple sample data (8 samples) of 20 children diagnosed with Duchenne muscular dystrophy. We evaluated the effect (variability) on the reported GFR from the different fitting methods and compared these results with GFR-values calculated from late samples only (samples after 120 minutes) and from one-sample methods. In 6 out of 20 cases, the fitting methods on the full concentration–time curve resulted in very different reported GFR-values, mainly because some methods were not able to fit the data, or methods resulted in GFR-values ranging from 0 to 120 mL/min. The reported GFR-result therefore strongly depends on the fitting method, making the full concentration–time method less robust than expected. Compared with a consensus reference GFR, the late sample models did not show fitting issues and may therefore be considered as more robust. Also the one-sample methods showed acceptable accuracy. The late sample methods (using 3 time-points) provide robust and reliable methods to determine GFR.
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spelling pubmed-87726272022-01-21 (51)Cr-EDTA plasma clearance in children: One, two, or multiple samples? Pottel, Hans Goffin, Karolien De Waele, Liesbeth Levtchenko, Elena Delanaye, Pierre Medicine (Baltimore) 5200 Plasma disappearance curves using multiple blood samples are a recognized reference method for measuring glomerular filtration rate (GFR). However, there is no consensus on the protocol for this type of measurement. A two-compartment model is generally considered acceptable for the mathematical description of the concentration–time decay curve. The impact of the fitting procedure on the reported GFR has not been questioned. We defined 8 different fitting procedures to calculate the area under the curve, and from this area under the curve, the GFR. We applied the 8 fitting methods (all considering a full concentration–time curve) on the multiple sample data (8 samples) of 20 children diagnosed with Duchenne muscular dystrophy. We evaluated the effect (variability) on the reported GFR from the different fitting methods and compared these results with GFR-values calculated from late samples only (samples after 120 minutes) and from one-sample methods. In 6 out of 20 cases, the fitting methods on the full concentration–time curve resulted in very different reported GFR-values, mainly because some methods were not able to fit the data, or methods resulted in GFR-values ranging from 0 to 120 mL/min. The reported GFR-result therefore strongly depends on the fitting method, making the full concentration–time method less robust than expected. Compared with a consensus reference GFR, the late sample models did not show fitting issues and may therefore be considered as more robust. Also the one-sample methods showed acceptable accuracy. The late sample methods (using 3 time-points) provide robust and reliable methods to determine GFR. Lippincott Williams & Wilkins 2022-01-21 /pmc/articles/PMC8772627/ /pubmed/35060531 http://dx.doi.org/10.1097/MD.0000000000028608 Text en Copyright © 2022 the Author(s). Published by Wolters Kluwer Health, Inc. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial License 4.0 (CCBY-NC), where it is permissible to download, share, remix, transform, and buildup the work provided it is properly cited. The work cannot be used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/)
spellingShingle 5200
Pottel, Hans
Goffin, Karolien
De Waele, Liesbeth
Levtchenko, Elena
Delanaye, Pierre
(51)Cr-EDTA plasma clearance in children: One, two, or multiple samples?
title (51)Cr-EDTA plasma clearance in children: One, two, or multiple samples?
title_full (51)Cr-EDTA plasma clearance in children: One, two, or multiple samples?
title_fullStr (51)Cr-EDTA plasma clearance in children: One, two, or multiple samples?
title_full_unstemmed (51)Cr-EDTA plasma clearance in children: One, two, or multiple samples?
title_short (51)Cr-EDTA plasma clearance in children: One, two, or multiple samples?
title_sort (51)cr-edta plasma clearance in children: one, two, or multiple samples?
topic 5200
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8772627/
https://www.ncbi.nlm.nih.gov/pubmed/35060531
http://dx.doi.org/10.1097/MD.0000000000028608
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