Cargando…
Near-Infrared Light-Triggered Generation of Reactive Oxygen Species and Induction of Local Hyperthermia from Indocyanine Green Encapsulated Mesoporous Silica-Coated Graphene Oxide for Colorectal Cancer Therapy
Near-infrared (NIR) light-mediated photothermal therapy (PTT) and photodynamic therapy (PDT) have widely been used for cancer treatment applications. However, a number of limitations (e.g., low NIR absorption capacity of photothermal agents, insufficient loading efficiency of photosensitive molecule...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8772730/ https://www.ncbi.nlm.nih.gov/pubmed/35052678 http://dx.doi.org/10.3390/antiox11010174 |
_version_ | 1784635911513833472 |
---|---|
author | Choi, Hyung Woo Lim, Jae Hyun Kim, Chan Woo Lee, Eunmi Kim, Jin-Moo Chang, Kiyuk Chung, Bong Geun |
author_facet | Choi, Hyung Woo Lim, Jae Hyun Kim, Chan Woo Lee, Eunmi Kim, Jin-Moo Chang, Kiyuk Chung, Bong Geun |
author_sort | Choi, Hyung Woo |
collection | PubMed |
description | Near-infrared (NIR) light-mediated photothermal therapy (PTT) and photodynamic therapy (PDT) have widely been used for cancer treatment applications. However, a number of limitations (e.g., low NIR absorption capacity of photothermal agents, insufficient loading efficiency of photosensitive molecules) have hindered the widespread use of NIR-mediated cancer therapy. Therefore, we developed a mesoporous silica-coated reduced graphene oxide (rGO) nanocomposite that could provide a high encapsulation rate of indocyanine green (ICG) and enhance PTT/PDT efficiency in vitro and in vivo. The ICG-encapsulated nanocomposite not only enhances the photothermal effect but also generates a large number of tumor toxic reactive oxygen species (ROS). By conjugation of polyethylene glycol (PEG) with folic acid (FA) as a tumor targeting moiety, we confirmed that ICG-encapsulated mesoporous silica (MS)-coated rGO nanocomposite (ICG@MS-rGO-FA) exhibited high colloidal stability and intracellular uptake in folate receptor-expressing CT-26 colorectal cancer cells. Upon NIR laser irradiation, this ICG@MS-rGO-FA nanocomposite induced the apoptosis of only CT-26 cells via enhanced PTT and PDT effects without any damage to normal cells. Furthermore, the ICG@MS-rGO-FA nanocomposite revealed satisfactory tumor targeting and biocompatibility in CT-26 tumor-bearing mice, thereby enhancing the therapeutic effects of PTT and PDT in vivo. Therefore, this tumor-targeted ICG@MS-rGO-FA nanocomposite shows a great potential for phototherapy applications. |
format | Online Article Text |
id | pubmed-8772730 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87727302022-01-21 Near-Infrared Light-Triggered Generation of Reactive Oxygen Species and Induction of Local Hyperthermia from Indocyanine Green Encapsulated Mesoporous Silica-Coated Graphene Oxide for Colorectal Cancer Therapy Choi, Hyung Woo Lim, Jae Hyun Kim, Chan Woo Lee, Eunmi Kim, Jin-Moo Chang, Kiyuk Chung, Bong Geun Antioxidants (Basel) Article Near-infrared (NIR) light-mediated photothermal therapy (PTT) and photodynamic therapy (PDT) have widely been used for cancer treatment applications. However, a number of limitations (e.g., low NIR absorption capacity of photothermal agents, insufficient loading efficiency of photosensitive molecules) have hindered the widespread use of NIR-mediated cancer therapy. Therefore, we developed a mesoporous silica-coated reduced graphene oxide (rGO) nanocomposite that could provide a high encapsulation rate of indocyanine green (ICG) and enhance PTT/PDT efficiency in vitro and in vivo. The ICG-encapsulated nanocomposite not only enhances the photothermal effect but also generates a large number of tumor toxic reactive oxygen species (ROS). By conjugation of polyethylene glycol (PEG) with folic acid (FA) as a tumor targeting moiety, we confirmed that ICG-encapsulated mesoporous silica (MS)-coated rGO nanocomposite (ICG@MS-rGO-FA) exhibited high colloidal stability and intracellular uptake in folate receptor-expressing CT-26 colorectal cancer cells. Upon NIR laser irradiation, this ICG@MS-rGO-FA nanocomposite induced the apoptosis of only CT-26 cells via enhanced PTT and PDT effects without any damage to normal cells. Furthermore, the ICG@MS-rGO-FA nanocomposite revealed satisfactory tumor targeting and biocompatibility in CT-26 tumor-bearing mice, thereby enhancing the therapeutic effects of PTT and PDT in vivo. Therefore, this tumor-targeted ICG@MS-rGO-FA nanocomposite shows a great potential for phototherapy applications. MDPI 2022-01-17 /pmc/articles/PMC8772730/ /pubmed/35052678 http://dx.doi.org/10.3390/antiox11010174 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Choi, Hyung Woo Lim, Jae Hyun Kim, Chan Woo Lee, Eunmi Kim, Jin-Moo Chang, Kiyuk Chung, Bong Geun Near-Infrared Light-Triggered Generation of Reactive Oxygen Species and Induction of Local Hyperthermia from Indocyanine Green Encapsulated Mesoporous Silica-Coated Graphene Oxide for Colorectal Cancer Therapy |
title | Near-Infrared Light-Triggered Generation of Reactive Oxygen Species and Induction of Local Hyperthermia from Indocyanine Green Encapsulated Mesoporous Silica-Coated Graphene Oxide for Colorectal Cancer Therapy |
title_full | Near-Infrared Light-Triggered Generation of Reactive Oxygen Species and Induction of Local Hyperthermia from Indocyanine Green Encapsulated Mesoporous Silica-Coated Graphene Oxide for Colorectal Cancer Therapy |
title_fullStr | Near-Infrared Light-Triggered Generation of Reactive Oxygen Species and Induction of Local Hyperthermia from Indocyanine Green Encapsulated Mesoporous Silica-Coated Graphene Oxide for Colorectal Cancer Therapy |
title_full_unstemmed | Near-Infrared Light-Triggered Generation of Reactive Oxygen Species and Induction of Local Hyperthermia from Indocyanine Green Encapsulated Mesoporous Silica-Coated Graphene Oxide for Colorectal Cancer Therapy |
title_short | Near-Infrared Light-Triggered Generation of Reactive Oxygen Species and Induction of Local Hyperthermia from Indocyanine Green Encapsulated Mesoporous Silica-Coated Graphene Oxide for Colorectal Cancer Therapy |
title_sort | near-infrared light-triggered generation of reactive oxygen species and induction of local hyperthermia from indocyanine green encapsulated mesoporous silica-coated graphene oxide for colorectal cancer therapy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8772730/ https://www.ncbi.nlm.nih.gov/pubmed/35052678 http://dx.doi.org/10.3390/antiox11010174 |
work_keys_str_mv | AT choihyungwoo nearinfraredlighttriggeredgenerationofreactiveoxygenspeciesandinductionoflocalhyperthermiafromindocyaninegreenencapsulatedmesoporoussilicacoatedgrapheneoxideforcolorectalcancertherapy AT limjaehyun nearinfraredlighttriggeredgenerationofreactiveoxygenspeciesandinductionoflocalhyperthermiafromindocyaninegreenencapsulatedmesoporoussilicacoatedgrapheneoxideforcolorectalcancertherapy AT kimchanwoo nearinfraredlighttriggeredgenerationofreactiveoxygenspeciesandinductionoflocalhyperthermiafromindocyaninegreenencapsulatedmesoporoussilicacoatedgrapheneoxideforcolorectalcancertherapy AT leeeunmi nearinfraredlighttriggeredgenerationofreactiveoxygenspeciesandinductionoflocalhyperthermiafromindocyaninegreenencapsulatedmesoporoussilicacoatedgrapheneoxideforcolorectalcancertherapy AT kimjinmoo nearinfraredlighttriggeredgenerationofreactiveoxygenspeciesandinductionoflocalhyperthermiafromindocyaninegreenencapsulatedmesoporoussilicacoatedgrapheneoxideforcolorectalcancertherapy AT changkiyuk nearinfraredlighttriggeredgenerationofreactiveoxygenspeciesandinductionoflocalhyperthermiafromindocyaninegreenencapsulatedmesoporoussilicacoatedgrapheneoxideforcolorectalcancertherapy AT chungbonggeun nearinfraredlighttriggeredgenerationofreactiveoxygenspeciesandinductionoflocalhyperthermiafromindocyaninegreenencapsulatedmesoporoussilicacoatedgrapheneoxideforcolorectalcancertherapy |