The Effectiveness of the Sexual Reproduction in Selected Clonal and Nonclonal Species of the Genus Ranunculus

SIMPLE SUMMARY: The genus Ranunculus (buttercup) includes over 600 species, some of which are endangered, e.g., Illyrian Buttercup. Knowledge of the reproductive biology of such species may be crucial for conservation action. For this purpose, six species with different reproduction modes (nonclonal reproducing sexually by seeds only, clonal propagating by seeds and additionally vegetatively and apomictic) were observed. Selected features related to the efficiency of sexual reproduction were described: pollen viability, number of fruit set, seed viability and germination. It has been shown that in clonal species, which include the Illyrian Buttercup, the efficiency of sexual reproduction is lower compared to nonclonal species. The results will support conservation action taken for this species. ABSTRACT: Generative processes have been evaluated in six European buttercup species in order to verify the hypothesis that the reproduction efficiency of clonal species is lower than that of nonclonal ones. The study covered common species (Ficaria verna, Ranunculus auricomus, R. bulbosus, R. cassubicus, R. lanuginosus) and the endangered R. illyricus. The following properties have been assessed: pollen viability (staining method), pollen grain germination and the pollen-tube elongation in pistil tissues (fluorescence microscopy), seed formation efficiency, seed viability (tetrazolium test) and germination ability by introducing factors interrupting dormancy (low temperature and gibberellin application). Additionally, the pistil morphology was documented for R. bulbosus, R. illyricus and R. cassubicus using SEM techniques. It was demonstrated that the reproductive efficiency, expressed as the production of viable seeds able to germinate, was significantly higher in the species reproducing sexually (especially in R. lanuginosus) compared to the clonal ones. However, the complexity observed leads to separation of an additional group (cluster) of apomictic species: R. auricomus and R. cassubicus, distinguished by the lowest pollen viability and a low ability of the seeds to germinate. In the vegetatively reproducing R. illyricus, the seed formation efficiency was just 13.2% despite the having highest number of pistils in its flowers. The developed seeds of this species observed in our experiment were viable, but in general effective methods to stimulate their germination have not been proposed yet. Here, the first comparative study concerning the biology of sexual reproduction of R. illyricus is presented in the context of its decreasing distribution in natural habitats.