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An In Vitro Evaluation of Denture Cleansing Regimens against a Polymicrobial Denture Biofilm Model

Denture stomatitis (DS) is an inflammatory disease resulting from a polymicrobial biofilm perturbation at the denture surface–palatal mucosa interface. Recommendations made by dental health care professionals often lack clarity for appropriate denture cleaning. This study investigated the efficacy o...

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Autores principales: Brown, Jason L., Young, Tracy, McKloud, Emily, Butcher, Mark C., Bradshaw, David, Pratten, Jonathan R., Ramage, Gordon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8772836/
https://www.ncbi.nlm.nih.gov/pubmed/35052990
http://dx.doi.org/10.3390/antibiotics11010113
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author Brown, Jason L.
Young, Tracy
McKloud, Emily
Butcher, Mark C.
Bradshaw, David
Pratten, Jonathan R.
Ramage, Gordon
author_facet Brown, Jason L.
Young, Tracy
McKloud, Emily
Butcher, Mark C.
Bradshaw, David
Pratten, Jonathan R.
Ramage, Gordon
author_sort Brown, Jason L.
collection PubMed
description Denture stomatitis (DS) is an inflammatory disease resulting from a polymicrobial biofilm perturbation at the denture surface–palatal mucosa interface. Recommendations made by dental health care professionals often lack clarity for appropriate denture cleaning. This study investigated the efficacy of brushing with off-the-shelf denture cleanser (DC) tablets (Poligrip(®)) vs. two toothpastes (Colgate(®) and Crest(®)) in alleviating the viable microorganisms (bacteria and fungi) in an in vitro denture biofilm model. Biofilms were grown on poly(methyl)methacrylate (PMMA) discs, then treated daily for 7 days with mechanical disruption (brushing), plus Poligrip(®) DC, Colgate(®) or Crest(®) toothpastes. Weekly treatment with Poligrip(®) DC on day 7 only was compared to daily modalities. All treatment parameters were processed to determine viable colony forming units for bacteria and fungi using the Miles and Misra technique, and imaged by confocal laser scanning microscopy (CLSM). Brushing with daily DC therapy was the most effective treatment in reducing the viable biofilm over 7 days of treatment. Brushing only was ineffective in controlling the viable bioburden, which was confirmed by CLSM imaging. This data indicates that regular cleansing of PMMA with DC was best for polymicrobial biofilms.
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spelling pubmed-87728362022-01-21 An In Vitro Evaluation of Denture Cleansing Regimens against a Polymicrobial Denture Biofilm Model Brown, Jason L. Young, Tracy McKloud, Emily Butcher, Mark C. Bradshaw, David Pratten, Jonathan R. Ramage, Gordon Antibiotics (Basel) Article Denture stomatitis (DS) is an inflammatory disease resulting from a polymicrobial biofilm perturbation at the denture surface–palatal mucosa interface. Recommendations made by dental health care professionals often lack clarity for appropriate denture cleaning. This study investigated the efficacy of brushing with off-the-shelf denture cleanser (DC) tablets (Poligrip(®)) vs. two toothpastes (Colgate(®) and Crest(®)) in alleviating the viable microorganisms (bacteria and fungi) in an in vitro denture biofilm model. Biofilms were grown on poly(methyl)methacrylate (PMMA) discs, then treated daily for 7 days with mechanical disruption (brushing), plus Poligrip(®) DC, Colgate(®) or Crest(®) toothpastes. Weekly treatment with Poligrip(®) DC on day 7 only was compared to daily modalities. All treatment parameters were processed to determine viable colony forming units for bacteria and fungi using the Miles and Misra technique, and imaged by confocal laser scanning microscopy (CLSM). Brushing with daily DC therapy was the most effective treatment in reducing the viable biofilm over 7 days of treatment. Brushing only was ineffective in controlling the viable bioburden, which was confirmed by CLSM imaging. This data indicates that regular cleansing of PMMA with DC was best for polymicrobial biofilms. MDPI 2022-01-16 /pmc/articles/PMC8772836/ /pubmed/35052990 http://dx.doi.org/10.3390/antibiotics11010113 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Brown, Jason L.
Young, Tracy
McKloud, Emily
Butcher, Mark C.
Bradshaw, David
Pratten, Jonathan R.
Ramage, Gordon
An In Vitro Evaluation of Denture Cleansing Regimens against a Polymicrobial Denture Biofilm Model
title An In Vitro Evaluation of Denture Cleansing Regimens against a Polymicrobial Denture Biofilm Model
title_full An In Vitro Evaluation of Denture Cleansing Regimens against a Polymicrobial Denture Biofilm Model
title_fullStr An In Vitro Evaluation of Denture Cleansing Regimens against a Polymicrobial Denture Biofilm Model
title_full_unstemmed An In Vitro Evaluation of Denture Cleansing Regimens against a Polymicrobial Denture Biofilm Model
title_short An In Vitro Evaluation of Denture Cleansing Regimens against a Polymicrobial Denture Biofilm Model
title_sort in vitro evaluation of denture cleansing regimens against a polymicrobial denture biofilm model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8772836/
https://www.ncbi.nlm.nih.gov/pubmed/35052990
http://dx.doi.org/10.3390/antibiotics11010113
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