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Effect of Doxycycline in Decreasing the Severity of Clostridioides difficile Infection in Mice

Background: Doxycycline possesses antibacterial activity against Clostridioides difficile and anti-inflammatory effects. Materials and Methods: The influence of doxycycline on the development of CDI was studied in an established animal model of CDI using C57BL/6 mice. Results: Mice intraperitoneally...

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Autores principales: Tsai, Bo-Yang, Lai, Yi-Hsin, Chiu, Chun-Wei, Hsu, Chih-Yu, Chen, Yi-Hsuan, Chen, Yueh-Lin, Tsai, Pei-Jane, Hung, Yuan-Pin, Ko, Wen-Chien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8772929/
https://www.ncbi.nlm.nih.gov/pubmed/35052993
http://dx.doi.org/10.3390/antibiotics11010116
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author Tsai, Bo-Yang
Lai, Yi-Hsin
Chiu, Chun-Wei
Hsu, Chih-Yu
Chen, Yi-Hsuan
Chen, Yueh-Lin
Tsai, Pei-Jane
Hung, Yuan-Pin
Ko, Wen-Chien
author_facet Tsai, Bo-Yang
Lai, Yi-Hsin
Chiu, Chun-Wei
Hsu, Chih-Yu
Chen, Yi-Hsuan
Chen, Yueh-Lin
Tsai, Pei-Jane
Hung, Yuan-Pin
Ko, Wen-Chien
author_sort Tsai, Bo-Yang
collection PubMed
description Background: Doxycycline possesses antibacterial activity against Clostridioides difficile and anti-inflammatory effects. Materials and Methods: The influence of doxycycline on the development of CDI was studied in an established animal model of CDI using C57BL/6 mice. Results: Mice intraperitoneally administered doxycycline had higher cecum weight (1.3 ± 0.1 vs. 0.5 ± 0.1 g; p < 0.001) and less body weight reduction (0.7 ± 0.5 g vs. −17.4 ± 0.2 g; p < 0.001) than untreated mice infected with C. difficile. Oral doxycycline, metronidazole, or vancomycin therapy resulted in less body weight reduction in mice with CDI than in untreated mice (1.1 ± 0.1 g, 1.3 ± 0.2 g, 1.2 ± 0.1 g, vs. 2.9 ± 0.3 g; p < 0.001). Doxycycline therapy led to lower expression levels of inflammatory cytokines, such as macrophage inflammatory protein-2 (0.4 ± 0.1 vs. 2.9 ± 1.3, p = 0.02), and higher levels of zonula occludens-1 (1.2 ± 0.1 vs. 0.8 ± 0.1, p = 0.02) in colonic tissues than in untreated mice. Conclusions: Concurrent intraperitoneal administration of doxycycline and oral C. difficile challenge does not aggravate the disease severity of CDI, and oral doxycycline may be a potential therapeutic option for CDI.
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spelling pubmed-87729292022-01-21 Effect of Doxycycline in Decreasing the Severity of Clostridioides difficile Infection in Mice Tsai, Bo-Yang Lai, Yi-Hsin Chiu, Chun-Wei Hsu, Chih-Yu Chen, Yi-Hsuan Chen, Yueh-Lin Tsai, Pei-Jane Hung, Yuan-Pin Ko, Wen-Chien Antibiotics (Basel) Article Background: Doxycycline possesses antibacterial activity against Clostridioides difficile and anti-inflammatory effects. Materials and Methods: The influence of doxycycline on the development of CDI was studied in an established animal model of CDI using C57BL/6 mice. Results: Mice intraperitoneally administered doxycycline had higher cecum weight (1.3 ± 0.1 vs. 0.5 ± 0.1 g; p < 0.001) and less body weight reduction (0.7 ± 0.5 g vs. −17.4 ± 0.2 g; p < 0.001) than untreated mice infected with C. difficile. Oral doxycycline, metronidazole, or vancomycin therapy resulted in less body weight reduction in mice with CDI than in untreated mice (1.1 ± 0.1 g, 1.3 ± 0.2 g, 1.2 ± 0.1 g, vs. 2.9 ± 0.3 g; p < 0.001). Doxycycline therapy led to lower expression levels of inflammatory cytokines, such as macrophage inflammatory protein-2 (0.4 ± 0.1 vs. 2.9 ± 1.3, p = 0.02), and higher levels of zonula occludens-1 (1.2 ± 0.1 vs. 0.8 ± 0.1, p = 0.02) in colonic tissues than in untreated mice. Conclusions: Concurrent intraperitoneal administration of doxycycline and oral C. difficile challenge does not aggravate the disease severity of CDI, and oral doxycycline may be a potential therapeutic option for CDI. MDPI 2022-01-17 /pmc/articles/PMC8772929/ /pubmed/35052993 http://dx.doi.org/10.3390/antibiotics11010116 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tsai, Bo-Yang
Lai, Yi-Hsin
Chiu, Chun-Wei
Hsu, Chih-Yu
Chen, Yi-Hsuan
Chen, Yueh-Lin
Tsai, Pei-Jane
Hung, Yuan-Pin
Ko, Wen-Chien
Effect of Doxycycline in Decreasing the Severity of Clostridioides difficile Infection in Mice
title Effect of Doxycycline in Decreasing the Severity of Clostridioides difficile Infection in Mice
title_full Effect of Doxycycline in Decreasing the Severity of Clostridioides difficile Infection in Mice
title_fullStr Effect of Doxycycline in Decreasing the Severity of Clostridioides difficile Infection in Mice
title_full_unstemmed Effect of Doxycycline in Decreasing the Severity of Clostridioides difficile Infection in Mice
title_short Effect of Doxycycline in Decreasing the Severity of Clostridioides difficile Infection in Mice
title_sort effect of doxycycline in decreasing the severity of clostridioides difficile infection in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8772929/
https://www.ncbi.nlm.nih.gov/pubmed/35052993
http://dx.doi.org/10.3390/antibiotics11010116
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