Cargando…

A Novel Method to Assess Antimicrobial Susceptibility in Commensal Oropharyngeal Neisseria—A Pilot Study

Commensal Neisseria provide a reservoir of resistance genes that can be transferred to the pathogens Neisseria gonorrhoeae and N. meningitidis in the human oropharynx. Surveillance programs are thus needed to monitor resistance in oropharyngeal commensal Neisseria, but currently the isolation and an...

Descripción completa

Detalles Bibliográficos
Autores principales: Laumen, Jolein Gyonne Elise, Abdellati, Saïd, Van Dijck, Christophe, Martiny, Delphine, De Baetselier, Irith, Manoharan-Basil, Sheeba Santhini, Van den Bossche, Dorien, Kenyon, Chris
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8772996/
https://www.ncbi.nlm.nih.gov/pubmed/35052976
http://dx.doi.org/10.3390/antibiotics11010100
_version_ 1784635975590215680
author Laumen, Jolein Gyonne Elise
Abdellati, Saïd
Van Dijck, Christophe
Martiny, Delphine
De Baetselier, Irith
Manoharan-Basil, Sheeba Santhini
Van den Bossche, Dorien
Kenyon, Chris
author_facet Laumen, Jolein Gyonne Elise
Abdellati, Saïd
Van Dijck, Christophe
Martiny, Delphine
De Baetselier, Irith
Manoharan-Basil, Sheeba Santhini
Van den Bossche, Dorien
Kenyon, Chris
author_sort Laumen, Jolein Gyonne Elise
collection PubMed
description Commensal Neisseria provide a reservoir of resistance genes that can be transferred to the pathogens Neisseria gonorrhoeae and N. meningitidis in the human oropharynx. Surveillance programs are thus needed to monitor resistance in oropharyngeal commensal Neisseria, but currently the isolation and antimicrobial susceptibility testing of these commensals is laborious, complex and expensive. In addition, the posterior oropharyngeal/tonsillar swab, which is commonly used to sample oropharyngeal Neisseria, is poorly tolerated by many individuals. We evaluated an alternative non-invasive method to isolate oropharyngeal commensal Neisseria and to detect decreased susceptibility to azithromycin using selective media (LBVT.SNR) with and without azithromycin (2 µg/mL). In this pilot study, we compared paired posterior oropharyngeal/tonsillar swabs and oral rinse-and-gargle samples from 10 participants and demonstrated that a similar Neisseria species diversity and number of colonies were isolated from both sample types. Moreover, the proportion of Neisseria colonies that had a decreased susceptibility to azithromycin was similar in the rinse samples compared to the swabs. This pilot study has produced encouraging data that a simple protocol of oral rinse-and-gargle and culture on plates selective for commensal Neisseria with and without a target antimicrobial can be used as a surveillance tool to monitor antimicrobial susceptibility in commensal oropharyngeal Neisseria. Larger studies are required to validate these findings.
format Online
Article
Text
id pubmed-8772996
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-87729962022-01-21 A Novel Method to Assess Antimicrobial Susceptibility in Commensal Oropharyngeal Neisseria—A Pilot Study Laumen, Jolein Gyonne Elise Abdellati, Saïd Van Dijck, Christophe Martiny, Delphine De Baetselier, Irith Manoharan-Basil, Sheeba Santhini Van den Bossche, Dorien Kenyon, Chris Antibiotics (Basel) Communication Commensal Neisseria provide a reservoir of resistance genes that can be transferred to the pathogens Neisseria gonorrhoeae and N. meningitidis in the human oropharynx. Surveillance programs are thus needed to monitor resistance in oropharyngeal commensal Neisseria, but currently the isolation and antimicrobial susceptibility testing of these commensals is laborious, complex and expensive. In addition, the posterior oropharyngeal/tonsillar swab, which is commonly used to sample oropharyngeal Neisseria, is poorly tolerated by many individuals. We evaluated an alternative non-invasive method to isolate oropharyngeal commensal Neisseria and to detect decreased susceptibility to azithromycin using selective media (LBVT.SNR) with and without azithromycin (2 µg/mL). In this pilot study, we compared paired posterior oropharyngeal/tonsillar swabs and oral rinse-and-gargle samples from 10 participants and demonstrated that a similar Neisseria species diversity and number of colonies were isolated from both sample types. Moreover, the proportion of Neisseria colonies that had a decreased susceptibility to azithromycin was similar in the rinse samples compared to the swabs. This pilot study has produced encouraging data that a simple protocol of oral rinse-and-gargle and culture on plates selective for commensal Neisseria with and without a target antimicrobial can be used as a surveillance tool to monitor antimicrobial susceptibility in commensal oropharyngeal Neisseria. Larger studies are required to validate these findings. MDPI 2022-01-13 /pmc/articles/PMC8772996/ /pubmed/35052976 http://dx.doi.org/10.3390/antibiotics11010100 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Laumen, Jolein Gyonne Elise
Abdellati, Saïd
Van Dijck, Christophe
Martiny, Delphine
De Baetselier, Irith
Manoharan-Basil, Sheeba Santhini
Van den Bossche, Dorien
Kenyon, Chris
A Novel Method to Assess Antimicrobial Susceptibility in Commensal Oropharyngeal Neisseria—A Pilot Study
title A Novel Method to Assess Antimicrobial Susceptibility in Commensal Oropharyngeal Neisseria—A Pilot Study
title_full A Novel Method to Assess Antimicrobial Susceptibility in Commensal Oropharyngeal Neisseria—A Pilot Study
title_fullStr A Novel Method to Assess Antimicrobial Susceptibility in Commensal Oropharyngeal Neisseria—A Pilot Study
title_full_unstemmed A Novel Method to Assess Antimicrobial Susceptibility in Commensal Oropharyngeal Neisseria—A Pilot Study
title_short A Novel Method to Assess Antimicrobial Susceptibility in Commensal Oropharyngeal Neisseria—A Pilot Study
title_sort novel method to assess antimicrobial susceptibility in commensal oropharyngeal neisseria—a pilot study
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8772996/
https://www.ncbi.nlm.nih.gov/pubmed/35052976
http://dx.doi.org/10.3390/antibiotics11010100
work_keys_str_mv AT laumenjoleingyonneelise anovelmethodtoassessantimicrobialsusceptibilityincommensaloropharyngealneisseriaapilotstudy
AT abdellatisaid anovelmethodtoassessantimicrobialsusceptibilityincommensaloropharyngealneisseriaapilotstudy
AT vandijckchristophe anovelmethodtoassessantimicrobialsusceptibilityincommensaloropharyngealneisseriaapilotstudy
AT martinydelphine anovelmethodtoassessantimicrobialsusceptibilityincommensaloropharyngealneisseriaapilotstudy
AT debaetselieririth anovelmethodtoassessantimicrobialsusceptibilityincommensaloropharyngealneisseriaapilotstudy
AT manoharanbasilsheebasanthini anovelmethodtoassessantimicrobialsusceptibilityincommensaloropharyngealneisseriaapilotstudy
AT vandenbosschedorien anovelmethodtoassessantimicrobialsusceptibilityincommensaloropharyngealneisseriaapilotstudy
AT kenyonchris anovelmethodtoassessantimicrobialsusceptibilityincommensaloropharyngealneisseriaapilotstudy
AT laumenjoleingyonneelise novelmethodtoassessantimicrobialsusceptibilityincommensaloropharyngealneisseriaapilotstudy
AT abdellatisaid novelmethodtoassessantimicrobialsusceptibilityincommensaloropharyngealneisseriaapilotstudy
AT vandijckchristophe novelmethodtoassessantimicrobialsusceptibilityincommensaloropharyngealneisseriaapilotstudy
AT martinydelphine novelmethodtoassessantimicrobialsusceptibilityincommensaloropharyngealneisseriaapilotstudy
AT debaetselieririth novelmethodtoassessantimicrobialsusceptibilityincommensaloropharyngealneisseriaapilotstudy
AT manoharanbasilsheebasanthini novelmethodtoassessantimicrobialsusceptibilityincommensaloropharyngealneisseriaapilotstudy
AT vandenbosschedorien novelmethodtoassessantimicrobialsusceptibilityincommensaloropharyngealneisseriaapilotstudy
AT kenyonchris novelmethodtoassessantimicrobialsusceptibilityincommensaloropharyngealneisseriaapilotstudy