Cargando…
Exploring the Nature of the Antimicrobial Metabolites Produced by Paenibacillus ehimensis Soil Isolate MZ921932 Using a Metagenomic Nanopore Sequencing Coupled with LC-Mass Analysis
The continuous emergence of multidrug-resistant (MDR) pathogens poses a global threat to public health. Accordingly, global efforts are continuously conducted to find new approaches to infection control by rapidly discovering antibiotics, particularly those that retain activities against MDR pathoge...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8773065/ https://www.ncbi.nlm.nih.gov/pubmed/35052889 http://dx.doi.org/10.3390/antibiotics11010012 |
_version_ | 1784635991757160448 |
---|---|
author | Eltokhy, Mohamed A. Saad, Bishoy T. Eltayeb, Wafaa N. Yahia, Ibrahim S. Aboshanab, Khaled M. Ashour, Mohamed S. E. |
author_facet | Eltokhy, Mohamed A. Saad, Bishoy T. Eltayeb, Wafaa N. Yahia, Ibrahim S. Aboshanab, Khaled M. Ashour, Mohamed S. E. |
author_sort | Eltokhy, Mohamed A. |
collection | PubMed |
description | The continuous emergence of multidrug-resistant (MDR) pathogens poses a global threat to public health. Accordingly, global efforts are continuously conducted to find new approaches to infection control by rapidly discovering antibiotics, particularly those that retain activities against MDR pathogens. In this study, metagenomic nanopore sequence analysis coupled with spectroscopic methods has been conducted for rapid exploring of the various active metabolites produced by Paenibacillus ehimensis soil isolate. Preliminary soil screening resulted in selection of a Gram-positive isolate identified via 16S ribosomal RNA gene sequencing as Paenibacillus ehimensis MZ921932. The isolate showed a broad range of activity against MDR Gram-positive, Gram-negative, and Candida spp. A metagenomics sequence analysis of the soil sample harboring Paenibacillus ehimensis isolate MZ921932 (NCBI GenBank accession PRJNA785410) revealed the presence of conserved biosynthetic gene clusters of petrobactin, tridecaptin, locillomycin (β-lactone), polymyxin, and macrobrevin (polyketides). The liquid chromatography/mass (LC/MS) analysis of the Paenibacillus ehimensis metabolites confirmed the presence of petrobactin, locillomycin, and macrobrevin. In conclusion, Paenibacillus ehimensis isolate MZ921932 is a promising rich source for broad spectrum antimicrobial metabolites. The metagenomic nanopore sequence analysis was a rapid, easy, and efficient method for the preliminary detection of the nature of the expected active metabolites. LC/MS spectral analysis was employed for further confirmation of the nature of the respective active metabolites. |
format | Online Article Text |
id | pubmed-8773065 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87730652022-01-21 Exploring the Nature of the Antimicrobial Metabolites Produced by Paenibacillus ehimensis Soil Isolate MZ921932 Using a Metagenomic Nanopore Sequencing Coupled with LC-Mass Analysis Eltokhy, Mohamed A. Saad, Bishoy T. Eltayeb, Wafaa N. Yahia, Ibrahim S. Aboshanab, Khaled M. Ashour, Mohamed S. E. Antibiotics (Basel) Article The continuous emergence of multidrug-resistant (MDR) pathogens poses a global threat to public health. Accordingly, global efforts are continuously conducted to find new approaches to infection control by rapidly discovering antibiotics, particularly those that retain activities against MDR pathogens. In this study, metagenomic nanopore sequence analysis coupled with spectroscopic methods has been conducted for rapid exploring of the various active metabolites produced by Paenibacillus ehimensis soil isolate. Preliminary soil screening resulted in selection of a Gram-positive isolate identified via 16S ribosomal RNA gene sequencing as Paenibacillus ehimensis MZ921932. The isolate showed a broad range of activity against MDR Gram-positive, Gram-negative, and Candida spp. A metagenomics sequence analysis of the soil sample harboring Paenibacillus ehimensis isolate MZ921932 (NCBI GenBank accession PRJNA785410) revealed the presence of conserved biosynthetic gene clusters of petrobactin, tridecaptin, locillomycin (β-lactone), polymyxin, and macrobrevin (polyketides). The liquid chromatography/mass (LC/MS) analysis of the Paenibacillus ehimensis metabolites confirmed the presence of petrobactin, locillomycin, and macrobrevin. In conclusion, Paenibacillus ehimensis isolate MZ921932 is a promising rich source for broad spectrum antimicrobial metabolites. The metagenomic nanopore sequence analysis was a rapid, easy, and efficient method for the preliminary detection of the nature of the expected active metabolites. LC/MS spectral analysis was employed for further confirmation of the nature of the respective active metabolites. MDPI 2021-12-22 /pmc/articles/PMC8773065/ /pubmed/35052889 http://dx.doi.org/10.3390/antibiotics11010012 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Eltokhy, Mohamed A. Saad, Bishoy T. Eltayeb, Wafaa N. Yahia, Ibrahim S. Aboshanab, Khaled M. Ashour, Mohamed S. E. Exploring the Nature of the Antimicrobial Metabolites Produced by Paenibacillus ehimensis Soil Isolate MZ921932 Using a Metagenomic Nanopore Sequencing Coupled with LC-Mass Analysis |
title | Exploring the Nature of the Antimicrobial Metabolites Produced by Paenibacillus ehimensis Soil Isolate MZ921932 Using a Metagenomic Nanopore Sequencing Coupled with LC-Mass Analysis |
title_full | Exploring the Nature of the Antimicrobial Metabolites Produced by Paenibacillus ehimensis Soil Isolate MZ921932 Using a Metagenomic Nanopore Sequencing Coupled with LC-Mass Analysis |
title_fullStr | Exploring the Nature of the Antimicrobial Metabolites Produced by Paenibacillus ehimensis Soil Isolate MZ921932 Using a Metagenomic Nanopore Sequencing Coupled with LC-Mass Analysis |
title_full_unstemmed | Exploring the Nature of the Antimicrobial Metabolites Produced by Paenibacillus ehimensis Soil Isolate MZ921932 Using a Metagenomic Nanopore Sequencing Coupled with LC-Mass Analysis |
title_short | Exploring the Nature of the Antimicrobial Metabolites Produced by Paenibacillus ehimensis Soil Isolate MZ921932 Using a Metagenomic Nanopore Sequencing Coupled with LC-Mass Analysis |
title_sort | exploring the nature of the antimicrobial metabolites produced by paenibacillus ehimensis soil isolate mz921932 using a metagenomic nanopore sequencing coupled with lc-mass analysis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8773065/ https://www.ncbi.nlm.nih.gov/pubmed/35052889 http://dx.doi.org/10.3390/antibiotics11010012 |
work_keys_str_mv | AT eltokhymohameda exploringthenatureoftheantimicrobialmetabolitesproducedbypaenibacillusehimensissoilisolatemz921932usingametagenomicnanoporesequencingcoupledwithlcmassanalysis AT saadbishoyt exploringthenatureoftheantimicrobialmetabolitesproducedbypaenibacillusehimensissoilisolatemz921932usingametagenomicnanoporesequencingcoupledwithlcmassanalysis AT eltayebwafaan exploringthenatureoftheantimicrobialmetabolitesproducedbypaenibacillusehimensissoilisolatemz921932usingametagenomicnanoporesequencingcoupledwithlcmassanalysis AT yahiaibrahims exploringthenatureoftheantimicrobialmetabolitesproducedbypaenibacillusehimensissoilisolatemz921932usingametagenomicnanoporesequencingcoupledwithlcmassanalysis AT aboshanabkhaledm exploringthenatureoftheantimicrobialmetabolitesproducedbypaenibacillusehimensissoilisolatemz921932usingametagenomicnanoporesequencingcoupledwithlcmassanalysis AT ashourmohamedse exploringthenatureoftheantimicrobialmetabolitesproducedbypaenibacillusehimensissoilisolatemz921932usingametagenomicnanoporesequencingcoupledwithlcmassanalysis |