Optimization of Propidium Monoazide qPCR (Viability-qPCR) to Quantify the Killing by the Gardnerella-Specific Endolysin PM-477, Directly in Vaginal Samples from Women with Bacterial Vaginosis

Quantification of the number of living cells in biofilm or after eradication treatments of biofilm, is problematic for different reasons. We assessed the performance of pre-treatment of DNA, planktonic cells and ex vivo vaginal biofilms of Gardnerella with propidium monoazide (PMAxx) to prevent qPCR...

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Detalles Bibliográficos
Autores principales: Latka, Agnieszka, Van Simaey, Leen, Reynders, Marijke, Cools, Piet, Rogier, Tess, Lebbe, Barbara, Corsini, Lorenzo, Landlinger, Christine, Vaneechoutte, Mario
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8773202/
https://www.ncbi.nlm.nih.gov/pubmed/35052988
http://dx.doi.org/10.3390/antibiotics11010111

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